| Literature DB >> 31988385 |
Molly Gasperini1, Jacob M Tome1, Jay Shendure2,3,4,5.
Abstract
The human gene catalogue is essentially complete, but we lack an equivalently vetted inventory of bona fide human enhancers. Hundreds of thousands of candidate enhancers have been nominated via biochemical annotations; however, only a handful of these have been validated and confidently linked to their target genes. Here we review emerging technologies for discovering, characterizing and validating human enhancers at scale. We furthermore propose a new framework for operationally defining enhancers that accommodates the heterogeneous and complementary results that are emerging from reporter assays, biochemical measurements and CRISPR screens.Entities:
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Year: 2020 PMID： 31988385 PMCID： PMC7845138 DOI： 10.1038/s41576-019-0209-0
Source DB: PubMed Journal: Nat Rev Genet ISSN： 1471-0056 Impact factor: 53.242