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Literature DB >> 3155715

Escherichia coli supH suppressor: temperature-sensitive missense suppression caused by an anticodon change in tRNASer2.

S Thorbjarnardóttir, H Uemura, T Dingermann, T Rafnar, S Thorsteinsdóttir, D Söll, G Eggertsson.

Abstract

We describe the cloning and the DNA sequence of the Escherichia coli supH missense suppressor and of the supD60(Am) suppressor genes. supH is a mutant form of serU which codes for tRNASer2. The supH coding sequence differs from the wild-type sequence by a single nucleotide change which corresponds to the middle position of the anticodon. The CGA anticodon of wild-type tRNA and CUA anticodon of supD tRNA is changed to CAA in supH tRNA, which is expected to recognize the UUG leucine codon. We propose that the supH suppressor causes the insertion of serine in response to this codon. The temperature sensitivity caused by supH may be due to a conformation of the CAA anticodon in the supH tRNASer that is slightly different than that in the corresponding tRNALeu species.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1985 PMID： 3155715 PMCID： PMC214857 DOI： 10.1128/jb.161.1.207-211.1985

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

42 in total

1. SUPPRESSION OF A COAT PROTEIN MUTANT OF THE BACTERIOPHAGE F2.

Authors: G W NOTANI; D L ENGELHARDT; W KONIGSBERG; N D ZINDER
Journal: J Mol Biol Date: 1965-06 Impact factor: 5.469

2. AMINO ACID SUBSTITUTIONS RESULTING FROM SUPPRESSION OF NONSENSE MUTATIONS. I. SERINE INSERTION BY THE SU-1 SUPPRESSOR GENE.

Authors: M G WEIGERT; A GAREN
Journal: J Mol Biol Date: 1965-06 Impact factor: 5.469

3. Mutations determining generalized resistance to aminoglycoside antibiotics in Escherichia coli.

Authors: S H Thorbjarnardóttir; R A Magnúsdóttir; G Eggertsson
Journal: Mol Gen Genet Date: 1978-04-25

4. Packaging recombinant DNA molecules into bacteriophage particles in vitro.

Authors: B Hohn; K Murray
Journal: Proc Natl Acad Sci U S A Date: 1977-08 Impact factor: 11.205

5. Studies on polynucleotides. LXXXV. Partial purification of an amber supressor tRNA and studies on in vitro suppression.

Authors: D Söll
Journal: J Mol Biol Date: 1968-05-28 Impact factor: 5.469

8. Molecular cloning, nucleotide sequence, and expression of shl, a new gene in the 2-minute region of the genetic map of Escherichia coli.

Authors: G Leclerc; G Noël; G R Drapeau
Journal: J Bacteriol Date: 1990-08 Impact factor: 3.490

9. Discrimination between glutaminyl-tRNA synthetase and seryl-tRNA synthetase involves nucleotides in the acceptor helix of tRNA.

Authors: M J Rogers; D Söll
Journal: Proc Natl Acad Sci U S A Date: 1988-09 Impact factor: 11.205

10. Nucleotide sequences of two serine tRNAs with a GGA anticodon: the structure-function relationships in the serine family of E. coli tRNAs.

Authors: H Grosjean; K Nicoghosian; E Haumont; D Söll; R Cedergren
Journal: Nucleic Acids Res Date: 1985-08-12 Impact factor: 16.971

Escherichia coli supH suppressor: temperature-sensitive missense suppression caused by an anticodon change in tRNASer2.

1. SUPPRESSION OF A COAT PROTEIN MUTANT OF THE BACTERIOPHAGE F2.

2. AMINO ACID SUBSTITUTIONS RESULTING FROM SUPPRESSION OF NONSENSE MUTATIONS. I. SERINE INSERTION BY THE SU-1 SUPPRESSOR GENE.

3. Mutations determining generalized resistance to aminoglycoside antibiotics in Escherichia coli.

4. Packaging recombinant DNA molecules into bacteriophage particles in vitro.

5. Studies on polynucleotides. LXXXV. Partial purification of an amber supressor tRNA and studies on in vitro suppression.

6. Genetic mapping and dominance of the amber suppressor, Su1 (supD), in Escherichia coli K-12.

7. Codon recognition by glycine transfer RNAs of Escherichia coli in vivo.

8. Anticodon shift in tRNA: a novel mechanism in missense and nonsense suppression.

9. Suppression in vitro: Identification of a Serine-sRNA as a "Nonsense" Suppressor.

10. Glutamic acid codon suppressors derived from a unique species of glycine transfer ribonucleic acid.

1. The Escherichia coli cell division mutation ftsM1 is in serU.

2. Nucleotide sequence of the ilvH-fruR gene region of Escherichia coli K12 and Salmonella typhimurium LT2.

Review 3. Structure and organization of the transfer ribonucleic acid genes of Escherichia coli K-12.

4. Temperature sensitivity caused by missense suppressor supH and amber suppressor supP in Escherichia coli.

Review 5. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

6. A minor arginine tRNA mutant limits translation preferentially of a protein dependent on the cognate codon.

Review 7. Linkage map of Escherichia coli K-12, edition 8.

8. Molecular cloning, nucleotide sequence, and expression of shl, a new gene in the 2-minute region of the genetic map of Escherichia coli.

9. Discrimination between glutaminyl-tRNA synthetase and seryl-tRNA synthetase involves nucleotides in the acceptor helix of tRNA.

10. Nucleotide sequences of two serine tRNAs with a GGA anticodon: the structure-function relationships in the serine family of E. coli tRNAs.