| Literature DB >> 31451685 |
Roberta Ragazzini1, Raquel Pérez-Palacios1, Irem H Baymaz2, Seynabou Diop1, Katia Ancelin1, Dina Zielinski1,3, Audrey Michaud1, Maëlle Givelet4, Mate Borsos5,6, Setareh Aflaki1, Patricia Legoix7, Pascal W T C Jansen2, Nicolas Servant1,3, Maria-Elena Torres-Padilla5,6, Deborah Bourc'his1, Pierre Fouchet4, Michiel Vermeulen2, Raphaël Margueron8.
Abstract
The Polycomb group of proteins is required for the proper orchestration of gene expression due to its role in maintaining transcriptional silencing. It is composed of several chromatin modifying complexes, including Polycomb Repressive Complex 2 (PRC2), which deposits H3K27me2/3. Here, we report the identification of a cofactor of PRC2, EZHIP (EZH1/2 Inhibitory Protein), expressed predominantly in the gonads. EZHIP limits the enzymatic activity of PRC2 and lessens the interaction between the core complex and its accessory subunits, but does not interfere with PRC2 recruitment to chromatin. Deletion of Ezhip in mice leads to a global increase in H3K27me2/3 deposition both during spermatogenesis and at late stages of oocyte maturation. This does not affect the initial number of follicles but is associated with a reduction of follicles in aging. Our results suggest that mature oocytes Ezhip-/- might not be fully functional and indicate that fertility is strongly impaired in Ezhip-/- females. Altogether, our study uncovers EZHIP as a regulator of chromatin landscape in gametes.Entities:
Mesh：
Substances：
Year: 2019 PMID： 31451685 PMCID： PMC6710278 DOI： 10.1038/s41467-019-11800-x
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919