Literature DB >> 3141328

Isolation and characterization of ras-transfected BALB/3T3 clone showing morphological transformation by 12-O-tetradecanoyl-phorbol-13-acetate.

K Sasaki1, H Mizusawa, M Ishidate.   

Abstract

The transformation frequency of mouse BALB/3T3 cells was significantly enhanced after transfection with an activated ras oncogene (v-Ha-ras) followed by treatment with a tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), suggesting that the ras oncogene acted as an initiator in two-stage carcinogenesis. A cell clone (Bhas42) containing the ras oncogene was isolated from the ras-transfected BALB/3T3 cells. Bhas42 cells were flat and showed contact inhibition, but the addition of TPA to quiescent Bhas42 cultures resulted in a dramatic change of cell morphology to spindle shape, doubling of the cell population, and increased DNA synthesis.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 3141328      PMCID： PMC5917616          DOI： 10.1111/j.1349-7006.1988.tb00056.x

Source DB:  PubMed          Journal:  Jpn J Cancer Res        ISSN： 0910-5050


  18 in total

1.  Alkali-labile colicinogenic factor E1 DNA molecules formed in the presence of N-methyl-N'-nitro-N-nitrosoguanidine.

Authors:  H Mizusawa; S Tanaka; M Kobayashi; K Koike
Journal:  Biochem Biophys Res Commun       Date:  1977-01-24       Impact factor: 3.575

2.  The function and mechanism of promoters of carcinogenesis.

Authors:  R K Boutwell
Journal:  CRC Crit Rev Toxicol       Date:  1974-01

3.  Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli.

Authors:  M Bazaral; D R Helinski
Journal:  J Mol Biol       Date:  1968-09-14       Impact factor: 5.469

4.  Oncogene-induced transformation of C3H 10T1/2 cells is enhanced by tumor promoters.

Authors:  W L Hsiao; S Gattoni-Celli; I B Weinstein
Journal:  Science       Date:  1984-11-02       Impact factor: 47.728

5.  DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.

Authors:  M Wigler; A Pellicer; S Silverstein; R Axel; G Urlaub; L Chasin
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

6.  Specific growth response of ras-transformed embryo fibroblasts to tumour promoters.

Authors:  G P Dotto; L F Parada; R A Weinberg
Journal:  Nature       Date:  1985 Dec 5-11       Impact factor: 49.962

7.  Two-stage chemical oncogenesis in cultures of C3H/10T1/2 cells.

Authors:  S Mondal; D W Brankow; C Heidelberger
Journal:  Cancer Res       Date:  1976-07       Impact factor: 12.701

8.  Effect of insulin on the transformation of BALB/3T3 cells by X-ray irradiation.

Authors:  M Umeda; K Tanaka; T Ono
Journal:  Gan       Date:  1983-12

9.  Differentiation of F9 teratocarcinoma stem cells after transfer of c-fos proto-oncogenes.

Authors:  R Müller; E F Wagner
Journal:  Nature       Date:  1984 Oct 4-10       Impact factor: 49.962

10.  Nucleotide sequence of the rightward operator of phage lambda.

Authors:  T Maniatis; A Jeffrey; D G Kleid
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205
View more
  7 in total

1.  Gene transfer into mammalian cells by rapid freezing.

Authors:  K Sasaki; H Mizusawa; M Ishidate; N Tanaka
Journal:  In Vitro Cell Dev Biol       Date:  1991-01

2.  Carcinogenic potential of tobacco tar-resistant Staphylococcus aureus in buccal cavity.

Authors:  H Fujiki; H Takeuchi; N Nishitani; H Yamanaka; K Suzuki; M Kurusu; M Suganuma
Journal:  J Cancer Res Clin Oncol       Date:  2004-03-11       Impact factor: 4.553

3.  Flat reversion by okadaic acid of raf and ret-II transformants.

Authors:  R Sakai; I Ikeda; H Kitani; H Fujiki; F Takaku; U Rapp; T Sugimura; M Nagao
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

4.  Incorrect strain information for mouse cell lines: sequential influence of misidentification on sublines.

Authors:  Kozue Uchio-Yamada; Fumio Kasai; Midori Ozawa; Arihiro Kohara
Journal:  In Vitro Cell Dev Biol Anim       Date:  2016-11-14       Impact factor: 2.416

5.  Deep neural network for the determination of transformed foci in Bhas 42 cell transformation assay.

Authors:  Minami Masumoto; Ittetsu Fukuda; Suguru Furihata; Takahiro Arai; Tatsuto Kageyama; Kiyomi Ohmori; Shinichi Shirakawa; Junji Fukuda
Journal:  Sci Rep       Date:  2021-12-02       Impact factor: 4.379

6.  Predicting Carcinogenic Mechanisms of Non-Genotoxic Carcinogens via Combined Analysis of Global DNA Methylation and In Vitro Cell Transformation.

Authors:  Sung-Hee Hwang; Hojin Yeom; Byeal-I Han; Byung-Joo Ham; Yong-Moon Lee; Mi-Ryung Han; Michael Lee
Journal:  Int J Mol Sci       Date:  2020-07-29       Impact factor: 5.923

7.  Gene Expression over Time during Cell Transformation Due to Non-Genotoxic Carcinogen Treatment of Bhas 42 Cells.

Authors:  Kiyomi Ohmori; Asuka Kamei; Yuki Watanabe; Keiko Abe
Journal:  Int J Mol Sci       Date:  2022-03-16       Impact factor: 5.923
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.