| Literature DB >> 31361603 |
William R McManus1, Michael J Bale1, Jonathan Spindler1, Ann Wiegand1, Andrew Musick1, Sean C Patro1, Michele D Sobolewski2, Victoria K Musick1, Elizabeth M Anderson1, Joshua C Cyktor2, Elias K Halvas2, Wei Shao3, Daria Wells3, Xiaolin Wu3, Brandon F Keele3, Jeffrey M Milush4, Rebecca Hoh4, John W Mellors2, Stephen H Hughes1, Steven G Deeks4, John M Coffin5, Mary F Kearney1.
Abstract
To investigate the possibility that HIV-1 replication in lymph nodes sustains the reservoir during ART, we looked for evidence of viral replication in 5 donors after up to 13 years of viral suppression. We characterized proviral populations in lymph nodes and peripheral blood before and during ART, evaluated the levels of viral RNA expression in single lymph node and blood cells, and characterized the proviral integration sites in paired lymph node and blood samples. Proviruses with identical sequences, identical integration sites, and similar levels of RNA expression were found in lymph nodes and blood samples collected during ART, and no single sequence with significant divergence from the pretherapy population was present in either blood or lymph nodes. These findings show that all detectable persistent HIV-1 infection is consistent with maintenance in lymph nodes by clonal proliferation of cells infected before ART and not by ongoing viral replication during ART.Entities:
Keywords: AIDS/HIV; Lymph; T cells
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Year: 2019 PMID： 31361603 PMCID： PMC6819093 DOI： 10.1172/JCI126714
Source DB: PubMed Journal: J Clin Invest ISSN： 0021-9738 Impact factor: 14.808