Literature DB >> 31267350

Feasibility of trifluoromethyl TROSY NMR at high magnetic fields.

Brittney A Klein1, Brian D Sykes2.   

Abstract

Insights into the structure and dynamics of large biological systems has been greatly improved by two concurrent NMR approaches: the application of transverse relaxation-optimized spectroscopy (TROSY) techniques in multi-dimensional NMR, especially the methyl-TROSY, and the resurgence of 19F NMR using trifluoromethyl (CF3) probes. Herein we investigate the feasibility of combining these approaches into a trifluoromethyl-TROSY experiment. Using a CF3-labelled parvalbumin, we have evaluated the natural abundance 13C-19F correlation spectra and find no indication of a CF3 TROSY at high magnetic fields.

Entities:  

Keywords:  19F NMR; Chemical shift anisotropy; High magnetic fields; Methyl-TROSY; Trifluoromethyl

Mesh：

Substances：

Year:  2019        PMID： 31267350     DOI： 10.1007/s10858-019-00266-0

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  17 in total

Review 1.  TROSY in NMR studies of the structure and function of large biological macromolecules.

Authors:  César Fernández; Gerhard Wider
Journal:  Curr Opin Struct Biol       Date:  2003-10       Impact factor: 6.809

2.  Fluorotyrosine alkaline phosphatase: internal mobility of individual tyrosines and the role of chemical shift anisotropy as a 19F nuclear spin relaxation mechanism in proteins.

Authors:  W E Hull; B D Sykes
Journal:  J Mol Biol       Date:  1975-10-15       Impact factor: 5.469

3.  Ca2+ and Mg2+ dependent conformations of troponin C as determined by 1H and 19F nuclear magnetic resonance.

Authors:  K B Seamon; D J Hartshorne; A A Bothner-By
Journal:  Biochemistry       Date:  1977-09-06       Impact factor: 3.162

Review 4.  Bringing dynamic molecular machines into focus by methyl-TROSY NMR.

Authors:  Rina Rosenzweig; Lewis E Kay
Journal:  Annu Rev Biochem       Date:  2014       Impact factor: 23.643

5.  Side-Chain Dynamics of the Trifluoroacetone Cysteine Derivative Characterized by 19F NMR Relaxation and Molecular Dynamics Simulations.

Authors:  Suad Rashid; Brian L Lee; Benjamin Wajda; Leo Spyracopoulos
Journal:  J Phys Chem B       Date:  2019-04-22       Impact factor: 2.991

6.  A new large-scale purification procedure for muscular parvalbumins.

Authors:  J Haiech; J Derancourt; J F Pechere; J G Demaille
Journal:  Biochimie       Date:  1979       Impact factor: 4.079

7.  Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution.

Authors:  K Pervushin; R Riek; G Wider; K Wüthrich
Journal:  Proc Natl Acad Sci U S A       Date:  1997-11-11       Impact factor: 11.205

Review 8.  Protein-Observed Fluorine NMR: A Bioorthogonal Approach for Small Molecule Discovery.

Authors:  Keith E Arntson; William C K Pomerantz
Journal:  J Med Chem       Date:  2015-12-14       Impact factor: 7.446

9.  Increased resolution of aromatic cross peaks using alternate 13C labeling and TROSY.

Authors:  Alexander G Milbradt; Haribabu Arthanari; Koh Takeuchi; Andras Boeszoermenyi; Franz Hagn; Gerhard Wagner
Journal:  J Biomol NMR       Date:  2015-05-10       Impact factor: 2.835

10.  Forbidden Coherence Transfer of 19F Nuclei to Quantitatively Measure the Dynamics of a CF₃-Containing Ligand in Receptor-Bound States.

Authors:  Yuji Tokunaga; Koh Takeuchi; Ichio Shimada
Journal:  Molecules       Date:  2017-09-07       Impact factor: 4.411
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  1 in total

1.  2'-O-Trifluoromethylated RNA - a powerful modification for RNA chemistry and NMR spectroscopy.

Authors:  Maximilian Himmelstoß; Kevin Erharter; Eva Renard; Eric Ennifar; Christoph Kreutz; Ronald Micura
Journal:  Chem Sci       Date:  2020-09-24       Impact factor: 9.825
  1 in total

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