Literature DB >> 3123895

Illegitimate recombination in Bacillus subtilis: nucleotide sequences at recombinant DNA junctions.

V I Bashkirov1, F K Khasanov, A A Prozorov.   

Abstract

The illegitimate integration of plasmid pGG20 (the hybrid between Staphylococcus aureus plasmid pE194 and Escherichia coli plasmid pBR322) into the Bacillus subtilis chromosome was studied. It was found that nucleotide sequences of both parental plasmids could be involved in this process. The recombinant DNA junctions between plasmid pGG20 and the chromosome were cloned and their nucleotide sequences were determined. The site of recombination located on the pBR322 moiety carried a short region (8 bp) homologous with the site on the chromosome. The nucleotide sequences of the pE194 recombination sites did not share homology with chromosomal sequences involved in the integration process. Two different pathways of illegitimate recombination in B. subtilis are suggested.

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Year:  1987        PMID： 3123895     DOI： 10.1007/bf00327215

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  16 in total

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Journal:  Mol Gen Genet       Date:  1978-11-09

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Journal:  FEBS Lett       Date:  1985-01-01       Impact factor: 4.124

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Journal:  Mol Gen Genet       Date:  1984

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Journal:  Mol Gen Genet       Date:  1977-06-08

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Journal:  Cell       Date:  1980-10       Impact factor: 41.582

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Authors:  H Ikeda
Journal:  Mol Gen Genet       Date:  1986-03

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Authors:  P E Love; R E Yasbin
Journal:  J Bacteriol       Date:  1984-12       Impact factor: 3.490

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Authors:  A A Prozorov; V I Bashkirov; F K Khasanov; E F Glumova; V Y Irich
Journal:  Gene       Date:  1985       Impact factor: 3.688
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  9 in total

1.  Integration of heterologous plasmid DNA into multiple sites on the genome of Campylobacter coli following natural transformation.

Authors:  P T Richardson; S F Park
Journal:  J Bacteriol       Date:  1997-03       Impact factor: 3.490

2.  Sequence specificity of Bacillus subtilis DNA gyrase in vivo.

Authors:  V I Bashkirov; D J Zvingila
Journal:  Genetica       Date:  1991       Impact factor: 1.082

3.  Integration of narrow-host-range vectors from Escherichia coli into the genomes of amino acid-producing corynebacteria after intergeneric conjugation.

Authors:  L M Mateos; A Schäfer; J Kalinowski; J F Martin; A Pühler
Journal:  J Bacteriol       Date:  1996-10       Impact factor: 3.490

4.  Homologous recombination between plasmid and chromosomal DNA in Bacillus subtilis requires approximately 70 bp of homology.

Authors:  F K Khasanov; D J Zvingila; A A Zainullin; A A Prozorov; V I Bashkirov
Journal:  Mol Gen Genet       Date:  1992-09

5.  Imprecise excision of plasmid pE194 from the chromosomes of Bacillus subtilis pE194 insertion strains.

Authors:  B Conrad; V I Bashkirov; J Hofemeister
Journal:  J Bacteriol       Date:  1992-11       Impact factor: 3.490

6.  Interplasmidic illegitimate recombination in Bacillus subtilis.

Authors:  V I Bashkirov; M M Stoilova-Disheva; A A Prozorov
Journal:  Mol Gen Genet       Date:  1988-08

7.  Identification of plasmid and Bacillus subtilis chromosomal recombination sites used for pE194 integration.

Authors:  L A Dempsey; D A Dubnau
Journal:  J Bacteriol       Date:  1989-05       Impact factor: 3.490

8.  Copy-choice illegitimate DNA recombination revisited.

Authors:  E d'Alençon; M Petranovic; B Michel; P Noirot; A Aucouturier; M Uzest; S D Ehrlich
Journal:  EMBO J       Date:  1994-06-01       Impact factor: 11.598

9.  Bacillus subtilis genome editing using ssDNA with short homology regions.

Authors:  Yang Wang; Jun Weng; Raza Waseem; Xihou Yin; Ruifu Zhang; Qirong Shen
Journal:  Nucleic Acids Res       Date:  2012-03-15       Impact factor: 16.971
  9 in total

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