Literature DB >> 105241

The relationship between molecular structure and transformation efficiency of some S. aureus plasmids isolated from B. subtilis.

U Canosi, G Morelli, T A Trautner.   

Abstract

DNA preparations of the chloramphenicol resistance determining S. aureus plasmids pC194, pC223, and PUB112 can be fractionated by gel electrophoresis into various bands. Electronmicroscopic investigations of these various molecular species obtained with pC194 indicated that, depending on the preparations, 70 to 80% of the molecules were monomers, while the rest consisted of various classes of concatemeric and/or interlocked multimers. Measurements of the specific transforming activity of the various molecular classes indicated that the monomers had less than one thousandth the activity of the multimeric plasmic DNA. pC194 DNA of high specific transforming activity could also be obtained by ligation of HindIII generated monomers into concatemeric DNA.

Entities:  

Mesh：

Substances：

Year:  1978        PMID： 105241     DOI： 10.1007/bf00267617

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  22 in total

1.  A freeze-squeeze method for recovering long DNA from agarose gels.

Authors:  R W Thuring; J P Sanders; P Borst
Journal:  Anal Biochem       Date:  1975-05-26       Impact factor: 3.365

2.  Studies on the structure and formation of polyoma DNA replicative intermediates.

Authors:  W Meinke; D A Goldstein
Journal:  J Mol Biol       Date:  1971-11-14       Impact factor: 5.469

3.  On the dose response in B. subtilis transfection: involvement of aggregates in phi 29 transfection.

Authors:  H Hirokawa; T A Trautner; G Lüder
Journal:  Mol Gen Genet       Date:  1977-11-18

4.  Transfecting deoxyribonucleic acid of Bacillus bacteriophage phi 29 that is protease sensitive.

Authors:  H Hirokawa
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205

5.  Physical properties of the DNA of bacteriophage SP50.

Authors:  N Biswal; A K Kleinschmidt; H C Spatz; T A Trautner
Journal:  Mol Gen Genet       Date:  1967

6.  Isolation of circular DNA by zonal centrifugation. Separation of normallength, double length and catenated M13 replicative form DNA and of host specific "episomal" DNA.

Authors:  R Jaenisch; P H Hofschneider; A Preuss
Journal:  Biochim Biophys Acta       Date:  1969-09-17

7.  Genetic and enzymic studies on the recombination process in Bacillus subtilis.

Authors:  G Mazza; A Fortunato; E Ferrari; U Canosi; A Falaschi; M Polsinelli
Journal:  Mol Gen Genet       Date:  1975

Review 8.  Transfection in B. subtilis.

Authors:  T A Trautner; H C Spatz
Journal:  Curr Top Microbiol Immunol       Date:  1973       Impact factor: 4.291

9.  Genetic and transfection studies with B, subtilis phage SP 50. I. Phage mutants with restricted growth on B. subtilis strain 168.

Authors:  E Rottländer; T A Trautner
Journal:  Mol Gen Genet       Date:  1970

10.  Mechanism of transfection with deoxyribonucleic acid from the temperate Bacillus bacteriophage phi-105.

Authors:  L Rutberg; J A Hoch; J Spizizen
Journal:  J Virol       Date:  1969-07       Impact factor: 5.103
View more
  93 in total

1.  Different specific activities of the monomeric and oligomeric forms of plasmid DNA in transformation of B. subtilis and E. coli.

Authors:  M Mottes; G Grandi; V Sgaramella; U Canosi; G Morelli; T A Trautner
Journal:  Mol Gen Genet       Date:  1979-07-24

2.  Peptidoglycan hydrolase LytF plays a role in cell separation with CwlF during vegetative growth of Bacillus subtilis.

Authors:  R Ohnishi; S Ishikawa; J Sekiguchi
Journal:  J Bacteriol       Date:  1999-05       Impact factor: 3.490

3.  Localization of the vegetative cell wall hydrolases LytC, LytE, and LytF on the Bacillus subtilis cell surface and stability of these enzymes to cell wall-bound or extracellular proteases.

Authors:  Hiroki Yamamoto; Shin-ichirou Kurosawa; Junichi Sekiguchi
Journal:  J Bacteriol       Date:  2003-11       Impact factor: 3.490

4.  The effect of restriction on shotgun cloning and plasmid stability in Bacillus subtilis Marburg.

Authors:  P Haima; S Bron; G Venema
Journal:  Mol Gen Genet       Date:  1987-09

5.  Bacillus subtilis RecO nucleates RecA onto SsbA-coated single-stranded DNA.

Authors:  Candela Manfredi; Begoña Carrasco; Silvia Ayora; Juan C Alonso
Journal:  J Biol Chem       Date:  2008-07-03       Impact factor: 5.157

Review 6.  Recombination-dependent concatemeric plasmid replication.

Authors:  J F Viret; A Bravo; J C Alonso
Journal:  Microbiol Rev       Date:  1991-12

7.  Characterization of the S1 binding site of the glutamic acid-specific protease from Streptomyces griseus.

Authors:  H R Stennicke; J J Birktoft; K Breddam
Journal:  Protein Sci       Date:  1996-11       Impact factor: 6.725

8.  Molecular cloning of heterologous chromosomal DNA by recombination between a plasmid vector and a homologous resident plasmid in Bacillus subtilis.

Authors:  T Gryczan; S Contente; D Dubnau
Journal:  Mol Gen Genet       Date:  1980-02

9.  Insertion-duplication mutagenesis in Streptococcus pneumoniae: targeting fragment length is a critical parameter in use as a random insertion tool.

Authors:  M S Lee; C Seok; D A Morrison
Journal:  Appl Environ Microbiol       Date:  1998-12       Impact factor: 4.792

10.  Generation of deletions in pneumococcal mal genes cloned in Bacillus subtilis.

Authors:  P Lopez; M Espinosa; B Greenberg; S A Lacks
Journal:  Proc Natl Acad Sci U S A       Date:  1984-08       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.