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Literature DB >> 3965303

A simple and rapid method for sequencing DNA.

Abstract

A simplified technique for DNA sequence analysis has been developed, based on modification of a previous method [(1980) Methods Enzymol. 65, 499-560]. It employs an adsorptive immobilization of terminally labelled DNA on DEAE paper followed by G, A+G, C+T and C specific modification and cleavage reactions. This solid-phase technique is faster and more convenient than the original method. The efficiency is comparable. The total processing time taken to produce cleaved fragments loaded on a gel is less than 2 h.

Entities: Chemical

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Year: 1985 PMID： 3965303 DOI： 10.1016/0014-5793(85)80185-x

Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124

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Cited

15 in total

1. Sequence specificity of Bacillus subtilis DNA gyrase in vivo.

Authors: V I Bashkirov; D J Zvingila
Journal: Genetica Date: 1991 Impact factor: 1.082

2. HIV-I protease. Cloning, expression, and purification.

Authors: N I Dergousova; A M Volynskaya; L D Rumsh
Journal: Appl Biochem Biotechnol Date: 1996 Oct-Nov Impact factor: 2.926

3. The upstream muscle-specific enhancer of the rat muscle creatine kinase gene is composed of multiple elements.

Authors: R A Horlick; P A Benfield
Journal: Mol Cell Biol Date: 1989-06 Impact factor: 4.272

4. Non-radioactive chemical sequencing of biotin labelled DNA.

Authors: P Richterich
Journal: Nucleic Acids Res Date: 1989-03-25 Impact factor: 16.971

5. Identification of a novel TA-rich DNA binding protein that recognizes a TATA sequence within the brain creatine kinase promoter.

Authors: G M Hobson; M T Mitchell; G R Molloy; M L Pearson; P A Benfield
Journal: Nucleic Acids Res Date: 1988-09-26 Impact factor: 16.971

6. Precise sequence assignment of replication origin in the control region of chick mitochondrial DNA relative to 5' and 3' D-loop ends, secondary structure, DNA synthesis, and protein binding.

Authors: M M Nass
Journal: Curr Genet Date: 1995-10 Impact factor: 3.886

A simple and rapid method for sequencing DNA.

1. Sequence specificity of Bacillus subtilis DNA gyrase in vivo.

2. HIV-I protease. Cloning, expression, and purification.

3. The upstream muscle-specific enhancer of the rat muscle creatine kinase gene is composed of multiple elements.

4. Non-radioactive chemical sequencing of biotin labelled DNA.

5. Identification of a novel TA-rich DNA binding protein that recognizes a TATA sequence within the brain creatine kinase promoter.

6. Precise sequence assignment of replication origin in the control region of chick mitochondrial DNA relative to 5' and 3' D-loop ends, secondary structure, DNA synthesis, and protein binding.

7. Brain and muscle creatine kinase genes contain common TA-rich recognition protein-binding regulatory elements.

8. B2-like repetitive sequence from the X chromosome of the American mink (Mustela vison).

9. Imprecise excision of plasmid pE194 from the chromosomes of Bacillus subtilis pE194 insertion strains.

10. Cloning and sequence of the human nuclear protein cyclin: homology with DNA-binding proteins.