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Literature DB >> 3118372

Multiple DNA sequence elements are necessary for the function of an immunoglobulin heavy chain promoter.

Abstract

Sequences required for the function of the mouse V1 immunoglobulin heavy chain variable-region (VH) promoter were identified by transient transfection of the normal and mutated promoters into plasmacytoma cells. Our results identify four regions required for normal promoter function: (i) the octamer ATGCAAAT, previously identified by others; (ii) a heptamer, CTAATGA; (iii) a pyrimidine-rich region; and (iv) a region between positions -125 and -251 relative to the transcription start site. Sequence analysis of 19 mouse and human VH 5' flanking regions shows that the heptamer and pyrimidine stretch are strongly conserved. We have also demonstrated that the octamer functions in an orientation independent manner in the VH promoter.

Entities: Chemical Gene Species

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Year: 1987 PMID： 3118372 PMCID： PMC299354 DOI： 10.1073/pnas.84.21.7634

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

56 in total

1. The nucleotide sequences of rearranged and germline immunoglobulin VH genes of a mouse myeloma MC101 and evolution of VH genes in mouse.

Authors: T Kataoka; T Nikaido; T Miyata; K Moriwaki; T Honjo
Journal: J Biol Chem Date: 1982-01-10 Impact factor: 5.157

2. Differences between germ-line and rearranged immunoglobulin V kappa coding sequences suggest a localized mutation mechanism.

Authors: M Pech; J Höchtl; H Schnell; H G Zachau
Journal: Nature Date: 1981-06-25 Impact factor: 49.962

3. Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.

Authors: H Sakano; R Maki; Y Kurosawa; W Roeder; S Tonegawa
Journal: Nature Date: 1980-08-14 Impact factor: 49.962

4. A mutant immunoglobulin light chain is formed by aberrant DNA- and RNA-splicing events.

Authors: J G Seidman; P Leder
Journal: Nature Date: 1980-08-21 Impact factor: 49.962

5. Variation in the crossover point of kappa immunoglobulin gene V-J recombination: evidence from a cryptic gene.

Authors: E E Max; J G Seidman; H Miller; P Leder
Journal: Cell Date: 1980-10 Impact factor: 41.582

6. Organization and complete sequence of identical embryonic and plasmacytoma kappa V-region genes.

Authors: Y Nishioka; P Leder
Journal: J Biol Chem Date: 1980-04-25 Impact factor: 5.157

7. Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors: A M Maxam; W Gilbert
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

8. IgG antibodies to phosphorylcholine exhibit more diversity than their IgM counterparts.

Authors: P J Gearhart; N D Johnson; R Douglas; L Hood
Journal: Nature Date: 1981-05-07 Impact factor: 49.962

9. Transcriptional enhancer elements in the mouse immunoglobulin heavy chain locus.

Authors: M Mercola; X F Wang; J Olsen; K Calame
Journal: Science Date: 1983-08-12 Impact factor: 47.728

10. Expression and regulation of immunoglobulin heavy chain gene transfected into lymphoid cells.

Authors: M S Neuberger
Journal: EMBO J Date: 1983 Impact factor: 11.598

41 in total

1. B cell development and immunoglobulin transcription in Oct-1-deficient mice.

Authors: Victoria E H Wang; Dean Tantin; Jianzhu Chen; Phillip A Sharp
Journal: Proc Natl Acad Sci U S A Date: 2004-02-04 Impact factor: 11.205

2. The Oct-1 POU domain mediates interactions between Oct-1 and other POU proteins.

Authors: C P Verrijzer; J A van Oosterhout; P C van der Vliet
Journal: Mol Cell Biol Date: 1992-02 Impact factor: 4.272

3. Identification of a novel factor that interacts with an immunoglobulin heavy-chain promoter and stimulates transcription in conjunction with the lymphoid cell-specific factor OTF2.

Authors: B K Yoza; R G Roeder
Journal: Mol Cell Biol Date: 1990-05 Impact factor: 4.272

Multiple DNA sequence elements are necessary for the function of an immunoglobulin heavy chain promoter.

1. The nucleotide sequences of rearranged and germline immunoglobulin VH genes of a mouse myeloma MC101 and evolution of VH genes in mouse.

2. Differences between germ-line and rearranged immunoglobulin V kappa coding sequences suggest a localized mutation mechanism.

3. Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.

4. A mutant immunoglobulin light chain is formed by aberrant DNA- and RNA-splicing events.

5. Variation in the crossover point of kappa immunoglobulin gene V-J recombination: evidence from a cryptic gene.

6. Organization and complete sequence of identical embryonic and plasmacytoma kappa V-region genes.

7. Sequencing end-labeled DNA with base-specific chemical cleavages.

8. IgG antibodies to phosphorylcholine exhibit more diversity than their IgM counterparts.

9. Transcriptional enhancer elements in the mouse immunoglobulin heavy chain locus.

10. Expression and regulation of immunoglobulin heavy chain gene transfected into lymphoid cells.

1. B cell development and immunoglobulin transcription in Oct-1-deficient mice.

2. The Oct-1 POU domain mediates interactions between Oct-1 and other POU proteins.

3. Identification of a novel factor that interacts with an immunoglobulin heavy-chain promoter and stimulates transcription in conjunction with the lymphoid cell-specific factor OTF2.

4. The immunoglobulin heavy-chain enhancer functions as the promoter for I mu sterile transcription.

5. Sequence of the VH gene for murine IgA X-24.

Review 6. The Oct1 transcription factor and epithelial malignancies: Old protein learns new tricks.

7. Identification of a matrix-associated region 5' of an immunoglobulin heavy chain variable region gene.

8. Regulation and a possible stage-specific function of Oct-2 during pre-B-cell differentiation.

9. Functional modularity in the SP6 kappa promoter.

10. A novel POU domain protein which binds to the T-cell receptor beta enhancer.