Ulas Oz1, Antonio Carlos Ruellas2,3, Philip M Westgate4, Lucia H Cevidanes5, Sarandeep S Huja6. 1. Department of Orthodontics, Near East University School of Dentistry, Nicosia, Northern Cyprus. 2. Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. 3. School of Dentistry, University of Michigan, Ann Arbor, Michigan. 4. Department of Biostatistics, University of Kentucky, Lexington, Kentucky. 5. Department of Orthodontics and Pediatric Dentistry, School of Dentistry, University of Michigan, Ann Arbor, Michigan. 6. Department of Orthodontics, James B. Edwards College of Dental Medicine, Medical University of South Carolina, Charleston, South Carolina.
Abstract
OBJECTIVES: The aim is to highlight a novel three-dimensional (3D) imaging methodology using micro-CT scans to visualize and measure bone modelling in an animal model. In order to validate the new methodology, we compared the 3D imaging method to traditional two-dimensional (2D) histomorphometry to assess growth changes in the jaws of a rodent. SETTING AND SAMPLE POPULATION: Rodent animal models. MATERIAL AND METHODS: Eleven rats were obtained from a larger previously published study. Sixty undecalcified histological sections from the maxilla and corresponding high-resolution in vivo micro-CT reconstructions were obtained. Bone modelling changes on specific alveolar surfaces were measured using traditional histomorphometry. Measurements of bone growth were also obtained via 3D Slicer software from 3D micro-CT generated models from the same plane containing the histological images. Both qualitative and quantitative 3D methods were compared to traditional histological measurements. Quantitative agreement between methods was categorized as follows: poor (>150 μm), good (150-100 μm) and excellent (<100 μm). RESULTS: Both qualitative (88.3%) and quantitative (86.7%) 3D measurements showed excellent agreement, when compared to histomorphometric measurements. Only 1.7% and 5% of the comparisons exhibited poor agreement (>150 μm) for qualitative and quantitative methods, respectively. DISCUSSION: The new 3D superimposition method compares very favourably with traditional histology. It is likely that in the future, such methods will be used in studies of bone adaptation. CONCLUSION: The 3D micro-CT qualitative and quantitative methods are reliable for measuring bone modelling changes and compare favourably to histology for the specific application described.
OBJECTIVES: The aim is to highlight a novel three-dimensional (3D) imaging methodology using micro-CT scans to visualize and measure bone modelling in an animal model. In order to validate the new methodology, we compared the 3D imaging method to traditional two-dimensional (2D) histomorphometry to assess growth changes in the jaws of a rodent. SETTING AND SAMPLE POPULATION: Rodent animal models. MATERIAL AND METHODS: Eleven rats were obtained from a larger previously published study. Sixty undecalcified histological sections from the maxilla and corresponding high-resolution in vivo micro-CT reconstructions were obtained. Bone modelling changes on specific alveolar surfaces were measured using traditional histomorphometry. Measurements of bone growth were also obtained via 3D Slicer software from 3D micro-CT generated models from the same plane containing the histological images. Both qualitative and quantitative 3D methods were compared to traditional histological measurements. Quantitative agreement between methods was categorized as follows: poor (>150 μm), good (150-100 μm) and excellent (<100 μm). RESULTS: Both qualitative (88.3%) and quantitative (86.7%) 3D measurements showed excellent agreement, when compared to histomorphometric measurements. Only 1.7% and 5% of the comparisons exhibited poor agreement (>150 μm) for qualitative and quantitative methods, respectively. DISCUSSION: The new 3D superimposition method compares very favourably with traditional histology. It is likely that in the future, such methods will be used in studies of bone adaptation. CONCLUSION: The 3D micro-CT qualitative and quantitative methods are reliable for measuring bone modelling changes and compare favourably to histology for the specific application described.
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