Involvement of Anopheles nili in Plasmodium falciparum transmission in North Benin.

BACKGROUND: Several studies carried out in Benin have shown the involvement of Anopheles gambiae sensu lato (s.l.), the Anopheles funestus group in malaria transmission, but none of them reported the contribution of the Anopheles nili group to the transmission of this disease. The current study investigated the question through an entomological cross-sectional survey performed in Northern Benin.
METHODS: Mosquito samplings were performed in September and October 2017 in 4 villages located in two districts: Bambaba and Wodara (Kérou district) and, Péhunco 2 and Béké (Péhunco district). The collections were carried out indoors and outdoors using human landing catches (HLC) to assess the human biting rate (HBR) and pyrethrum spray catches (PSC) to evaluate the blood feeding rate and the blood meal origin using the ELISA test. All collected mosquitoes were morphologically identified and, the polymerase chain reaction (PCR) technique was used for molecular identification of sibling species of An. gambiae s.l., An. funestus group and An. nili group sporozoite index (SI) was also assessed by the ELISA test.
RESULTS: Overall, An. gambiae s.l., An. funestus group and An. nili group were the three vectors found in the study area. A significantly higher human biting rate (HBR) was recorded in An. nili group (5 bites/human/night) compared to An. funestus group (0.656 bites/human/night) in the Kérou district (p < 0.0001). Anopheles gambiae s.l. displayed the highest HBR (26.19 bites/human/night) in the same district. The entomological inoculation rate (EIR) was 1.875 infected bites/human/month in An. nili group against 13.05 infected bites/human/month in An. gambiae s.l. and 0.938 infected bites/human/month in An. funestus group in Kérou. In Péhunco, the EIR was 1.02 infected bites/human/month in An. gambiae s.l. PCR results showed that An. nili sensu stricto (s.s.) and An. funestus s.s. were the only species of the An. nili and An. funestus groups, respectively. The anthropophagic character of An. gambiae s.l. was also highlighted.
CONCLUSION: This study provides useful information on the contribution of An. nili group as secondary vector to malaria transmission in northern Benin. Broader studies must also be carried out in a larger study area to assess the involvement of other Anopheles species to malaria transmission. This will aid to better plan malaria vector control interventions.

Background

A wide range of human and animal diseases are transmitted by mosquitoes and those diseases remain a major source of death worldwide [1]. Malaria remains one of the most serious vector-borne diseases, affecting half of the world’s 7.4 billion people [2]. The proliferation of mosquitoes is promoted not only by ecological changes due to human activities (deforestation, public works, construction of dams, rice paddies, irrigation), but also by environmental parameters (rainfall, temperature and relative humidity), which also play a fundamental role in the level of transmission and the epidemiology of diseases [3, 4]. In intertropical Africa, malaria transmission is very heterogeneous due to eco-climatic variations [5]. Currently, five species of the parasite of the Plasmodium genus have been identified as responsible for malaria infection in humans [6]. Among them Plasmodium falciparum remains the most virulent species causing the deadly forms of malaria [7]. The Plasmodium species responsible for human malaria are mainly transmitted by primary vector species, such as Anopheles gambiae sensu lato (s.l.), Anopheles funestus group and Anopheles nili group [8, 9]. The existence of those different species complexes in an area represents a great challenge for malaria control programmes. Anopheles nili group has a wide geographical distribution in most of tropical Africa [10] and its preferential habitats are fast-flowing, upright-flowing streams, large rivers or dense shade along streams [7]. It is a group that includes four species, including An. nili sensu stricto (s.s.), Anopheles carnevalei, Anopheles somalicus and Anopheles ovengensis [8, 11].

In Benin, over the past two decades, the roles of An. gambiae s.l. and An. funestus group in the transmission of P. falciparum in several regions of the country have been studied by several authors [12-19]. Few of these studies have shown the presence of An. nili group in the different surveyed areas. Moreover, none of them has demonstrated its involvement in malaria transmission in Benin. It is in this context that a large population of An. nili group was collected during this cross-sectional survey conducted in the districts of Kérou and Péhunco located in the Atacora region. Thus, the possible involvement of this Anopheles species in the transmission of P. falciparum was investigated in the study area.

This study aims at providing information on vector species composition involved in malaria transmission in the areas of Kérou and Péhunco (northwestern Benin) where there is a lack of data.

Methods

Study area

This study was conducted from September to October 2017 in the districts of Kérou and Péhunco, both located in the north-western Benin (Fig. 1). The two districts have a Sudano-Guinean climate characterized by a rainy season, from mid-April to mid-October, and a dry season from mid-October to mid-April. They belong to an agroecological zone characterized by an average annual rainfall of 1000 mm. The average temperature varies between 25 in August and 31 °C in April.

Fig. 1

Map of the study area

The district of Kérou is irrigated by two large permanent rivers, the Mekrou and the Pendjari as well as of many tributaries from the Alibori river. Péhunco is drained by the river Mekrou and also by many rivers. The most important economic activity of these two districts is agriculture, especially with the cultivation of cotton, sorghum and cashew nuts [20].

Mosquito sampling and processing on the field

In each district, two villages were selected for mosquito collections, Wodara and Bambaba (respectively central and peripheral village of the Kérou district) and, Péhunco 2 and Béké (respectively central and peripheral village of the Péhunco district). Collections of mosquitoes were performed using both human landing catches (HLC) and pyrethrum spray catches (PSC).

HLCs were carried out in two houses per village. Four sessions of night collections were organized each month with one human sitting inside and another one outside of each house, bringing to a total of 16 human-nights/village/month and a total of 32 human-nights/district/month.

For PSCs, 10 bedrooms were surveyed in each district (5 in the central village and the remaining 5 in the peripheral village) to collect early in the mornings, all mosquitoes that have entered the houses the night before. Thus, aerosol bombs (Rambo®) containing 0.25% transfluthrin and 0.20% permethrin were sprayed in the houses and white canvas were spread on the floor for the collection of fallen mosquitoes.

Mosquitoes caught by both methods were morphologically identified using a mosquito identification key [21] and, the ovaries of the Anopheles vectors were dissected to determine the parity rate [22]. Anopheles vectors collected by PSCs were classified according to the physiological state of their abdomens (unfed, fed, half gravid, gravid) to determine the blood feeding rate. The Anopheles vectors collected with the two sampling methods were then stored in tagged eppendorf tubes containing silica gel and cotton for subsequent laboratory analysis.

Laboratory analysis

539 head-thoraxes of Anopheles vectors collected indoor and outdoor through HLC were crushed and then analysed by the ELISA tests for the determination of the circumsporozoite protein (CSP) positivity of Plasmodium falciparum using the protocol of Wirtz et al. [23]. This allows the determination of the sporozoite index (SI). The legs, wings, and abdomens of these mosquitoes were used for DNA extraction to perform molecular species identification.

The PCR technique based on the protocols of Santomalazza et al. [24], Koekemoer et al. [25] and Kengne et al. [26] was used to identify the sibling species of An. gambiae complex, An. funestus group and An. nili group, respectively. In An. gambiae s.l., the presence of L1014F kdr and G119S ace-1 mutations was determined following the methods of Martinez-Torres et al. [27] and Weill et al. [28], respectively. Moreover, the blood meal origin was sought in the blood fed Anopheles mosquitoes collected by PSC, using a direct enzyme immunoassay (ELISA) according to the method of Beier et al. [29] with human, cattle, sheep, chicken and pig antibodies.

Data analysis

The results were processed and analysed using the R Core Team software (Version 3.5.1-2018) and Excel spreadsheets. The human biting rates (HBR = number of collected vectors/number of humans/number of nights), infection rates (Number of infected mosquitoes/total tested), entomological inoculation rates (EIRnight = HBR × infection rate; EIRmonth = EIRnight × 30) and parity rates (Number of parous mosquitoes/total tested) were calculated and compared between species of the same district. The Poisson test allowed us to compare the HBRs and EIRs between species in the district of Kérou. The comparison of parity rates, infectivity and allelic frequencies of L1014F kdr and G119S ace-1 mutations by species and by site were made with the Chi square comparison test.

Results

Mosquito species composition and molecular identification of sibling species of Anopheles gambiae complex, Anopheles funestus group and Anopheles nili group

The mosquito fauna collected in both surveyed districts was very diverse (Table 1). A total of 1558 mosquitoes divided into 12 different species were collected with a predominance of Anopheles species (83.25%: 1297/1558).

Table 1

Mosquito species composition in Péhunco and Kérou between September and October 2017

Species	Péhunco		Kérou		Total
	Indoor	Outdoor	Indoor	Outdoor
Anopheles gambiae s.l.	74	54	430	408	966
Anopheles funestus group	0	1	9	12	22
Anopheles nili group	0	0	51	109	160
Anopheles pharoensis	0	0	0	1	1
Anopheles ziemanni	0	0	14	33	47
Anopheles brohieri	0	0	19	82	101
Anophelinae (sub total 1)	74	55	523	645	1297
Aedes aegypti	0	0	1	0	1
Aedes vitatus	0	0	0	2	2
Culex quinquefasciatus	127	91	18	16	252
Culex nebulosus	0	2	0	0	2
Culex tigripes	1	0	0	0	1
Mansonia africana	1	1	0	1	3
Culicinae (sub total 2)	129	94	19	19	261
Grand total	203	149	542	664	1558

In Péhunco, out of the collected mosquitoes, 36.36% (128/352) were An. gambiae s.l. and 0.3% (1/352) An. funestus group. In the Kérou district, 9 species were collected. Among those species, An. gambiae s.l. was the most abundant (69.48%: 838/1206) followed by An. nili group (13.27%: 160/1206), Anopheles brohieri (8.37%: 101/1206), Anopheles ziemanni (3.90%: 47/1206) and An. funestus group (1.74%: 21/1206).

PCR results showed the presence of An. gambiae and An. coluzzii as sibling species of the An. gambiae complex in both Péhunco and Kérou. Anopheles gambiae was found in majority (96.43% and 78.43% at Péhunco and Kérou, respectively). In addition, all mosquito specimens of the An. funestus group (Péhunco and Kérou) and the An. nili group (Kérou) were found to be An. funestus s.s. and An. nili s.s., respectively (Fig. 2).

Fig. 2

Distribution of sibling species within the An. gambiae complex and the An. funestus and An. nili groups in the study sites

Biting behaviour, HBR, SI and EIR

In Kérou, the An. nili group was exophagic as the proportion of this species caught outdoors (68.13%: 109/160) was significantly higher than that recorded indoors (31.87%: 51/160) (p < 0.0001). In the same district the indoor (51.31%: 430/838) and the outdoor (48.69%: 408/838) biting behaviour of An. gambiae s.l. was similar (p = 0.282), as was also observed for the An. funestus group [indoors proportion: 42.86% (9/21); outdoors proportion: 57.14% (12/21); p = 0.35]. However, in Pehunco, An. gambiae s.l. was endophagic [Indoors proportion: 57.81% (74/128); Outdoors proportion: 42.19% (54/128); p = 0.011] (Table 1).

In the district of Kérou, the biting rate of An. gambiae s.l. (26 bites/human/night) was significantly higher than that of the An. nili group (5 bites/human/night) (p < 0.0001). The biting rate of An. funestus group (0.656 bites/human/night) was significantly lower than that of An. gambiae s.l. and An. nili group (p < 0.0001). The trend was the same between An. funestus group and An. gambiae s.l. in Péhunco (p < 0.0001) (Table 2).

Table 2

Entomological inoculation rate (EIR) in Anopheles gambiae s.l., Anopheles funestus group and Anopheles nili group in the Péhunco and Kérou districts

	Biting locations	Number of collected mosquitoes		HBR (b/h/night)		Sporozoite index (N tested)		EIR (ib/h/night)		EIR (ib/h/month)
		Kérou	Péhunco	Kérou	Péhunco	Kérou	Péhunco	Kérou	Péhunco	Kérou	Péhunco
An. gambiae s.l.	Indoors	430	74	26.875a	4.625a	0.025 (121)a	0.014 (66)a	0.672	0.065	20.156a	1.943a
	Outdoors	408	54	25.5a	3.375a	0.008 (120)a	0 (50)a	0.204	0	6.120b	0b
	Total	838	128	26.19*	4*	0.017* (241)	0.009 (116)	0.435	0.034	13.05*	1.02
An. funestus group	Indoors	9	0	0.5625b	0a	0.111 (9)a	0	0.062	0	1.873c	0
	Outdoors	12	1	0.75b	0.0625a	0 (12)a	0 (1)	0	0	0d	0
	Total	21	1	0.656‡	0.031‡	0.048 (21)*	0 (1)	0.031	0	0.9375‡	0
An. nili group	Indoors	51	0	3.1875c	0	0.02 (51)a	0	0.064	0	1.913e	0
	Outdoors	109	0	6.8125d	0	0.009 (109)a	0	0.061	0	1.839e	0
	Total	160	0	5¥	0	0.0125 (160)*	0	0.063	0	1.875¥	0

b/h, bite/human; ib/h, infected bite/human; N, number of mosquitoes

a,b,c,d,eValues with different superscripts at the two biting locations of the same district are significantly different (p < 0.05)

*,‡,¥The total values with different superscripts in a same district are significantly different (p < 0.05)

For the SI, no significant difference was recorded between the three vectors in the Kérou district (p = 0.496). In Péhunco, only An. gambiae s.l. was found positive for P. falciparum antigen (Table 2).

In Kérou, the indoors EIR of An. gambiae s.l. (20.156 infected bites/human/month) and An. funestus group (1.873 infected bites/human/month) were significantly higher than that recorded outdoors [An. gambiae s.l.: 6.12 infected bites/human/month (p < 0.0001); An. funestus group: 0 infected bites/human/month (p = 0.0005)]. In the same district, the indoor (1.913 infected bites/human/month) and the outdoor (1.839 infected bites/human/month) EIR of An. nili group was similar (p = 1). Furthermore, in the district of Kérou, the malaria transmission risk by An. gambiae s.l. (13.05 infected bites/human/month) was significantly higher than the malaria transmission risk of the An. nili group (1.875 infectious bites/human/month) (p = 0.0009) and the An. funestus group (0.938 infected bites/human/month) (p < 0.0001). It should be noted that the EIR of An. nili group was significantly higher than that of An. funestus group in Kérou (p < 0.0001) during the study period. In Péhunco, the indoor EIR for An. gambiae s.l. (1.943 infected bites/human/month) was significantly higher than that noted outdoor (0 infected bites per month) (p < 0.0001) (Table 2).

Parity rate of An. gambiae s.l., An. funestus group and An. nili group

Figure 3 shows the different parity rates with 88% [78.99–97.01], 57.14% [35.97–78.31] and 46% [32.19–59.81] for An. gambiae s.l., An. funestus group and An. nili group in the Kérou district, respectively. The parity rate in An. gambiae s.l. was significantly higher than that of An. funestus group (p = 0.004) and An. nili group (p < 0.0001). In Pehunco, the parity rate recorded for An. gambiae s.l. was 72% [59.55–84.45].

Fig. 3

Parity rate in An. gambiae s.l., An. funestus s.s. and An. nili s.s. collected in Kérou and Péhunco

Blood feeding rate and blood meal source in An. gambiae s.l

The blood feeding rate of An. gambiae s.l. collected inside houses after PSC was 63.16% in Péhunco against 60.53% in Kérou (p = 0.847). In both districts, the majority of An. gambiae s.l. vectors took their blood meal on human (91.30% in Kérou against 100% in Péhunco). In Kérou, 4.35% (1/23) of the collected An. gambiae s.l. blood fed on beef and, the same proportion on sheep (Table 3).

Table 3

Blood meal origin in Anopheles gambiae s.l. collected indoors in Kérou and Péhunco

Districts	T. collected	T.Blood fed	Bloodfeeding rate(%)	Blood meal origin in An. gambiae s.l.
				N. Human (%)	N. Beef(%)	N. Sheep(%)	N. Pig(%)
Péhunco	19	12	63.16	12 (100)			0
Kérou	38	23	60.53	21 (91.30)	1 (4.35)	1 (4.35)	0
Total	57	35	61.40	22 (62.86)	1 (2.86)	1 (2.86)	0

T, total of mosquitoes; N., number of mosquitoes having taken their blood meal on

L1014F kdr and G119S ace-1 allele frequencies in An. gambiae and An. coluzzii

The L1014F kdr mutation was found at very high frequencies in both Péhunco (86% and 100% respectively in An. gambiae and An. coluzzii) and Kérou (83% and 73% respectively in An. gambiae and An. coluzzii) (Table 4). In each district, the allelic frequencies of the L1014F kdr mutation were similar in An. gambiae and An. coluzzii (p > 0.05).

Table 4

Allelic frequencies of L1014F kdr and G119S ace-1 mutations in Anopheles gambiae and Anopheles coluzzii in the Péhunco and Kérou districts

	Species	N tested	L1014F Kdr				p value	G119S Ace-1				p-value
			RR	RS	SS	F(L1014F Kdr)		RR	RS	SS	F(G119S Ace-1)
Péhunco	An. gambiae	54	42	9	3	0.86	0.957	0	5	49	0.05	1
	An. coluzzii	2	2	0	0	1		0	0	2	0
Kérou	An. gambiae	41	30	8	3	0.83	0.439	0	6	35	0.073	1
	An. coluzzii	11	7	2	2	0.73		0	1	10	0.05

The G119S ace-1 mutation was also recorded in the two districts but at low levels with allelic frequencies of 5% and 0%, respectively, in An. gambiae and An. coluzzii in Péhunco and, 7.3% and 5%, respectively, in An. gambiae and An. coluzzii in Kérou (Table 4). No significant difference was also recorded between the allelic frequencies of the G119S ace-1 mutation of An. coluzzii and An. gambiae in both districts (p > 0.05).

Discussion

The study of malaria vectors in an area is a prerequisite not only to understand the epidemiology of the disease but also to implement a strategy for effective and targeted control of those vectors. In the current study, 12 mosquito species including 6 anophelinae were identified in the two surveyed districts. Among these 6 Anopheles species, two (An. gambiae s.l. and An. funestus group) have already been implicated in malaria transmission in Benin [13, 17, 18]. During this cross-sectional study, they were found in both study districts but, An. nili group was found only in Kérou. The three vector species (An. gambiae s.l., An. funestus group and An. nili group) identified in Kérou live in sympatry in this savannah zone. This finding is similar to those of several authors in West Africa [30, 31].

In Péhunco and Kérou, An. gambiae and An. coluzzii are the only members of the An. gambiae complex recorded with a predominance of An. gambiae (96.43% and 78.43%, respectively, in Péhunco and Kérou). No species of An. arabiensis was identified after PCR in both districts. This could be due to anthropogenic actions such as deforestation and urbanization that destroy its natural habitats causing its gradual disappearance in some areas of northern Benin as reported by Aïkpon et al. [32] and Salako et al. [33]. All the specimens of the An. nili and An. funestus groups identified by PCR were An. nili s.s. and An. funestus s.s. in the district of Kérou. These results corroborate those of Djouaka et al. [19, 34] who found An. funestus s.s. as the only species of the An. funestus group in the areas of Pahou and Kpomè in southern Benin. The results are also similar to works by Carnevale et al. [35], Dia et al. [36] and Adja et al. [31], who found An. nili s.s. as the only species in forest zone respectively in Cameroon, Senegal and Côte d’Ivoire.

The number of An. funestus group collected in both districts was low during the study period as was also reported by some authors in the area and around [32, 37, 38]. This could be justified by the scarcity of breeding sites favorable to the development of this species. Moreover, contrary to An. gambiae s.l., An. nili group was found to be exophagic (significantly higher biting activity outdoors compared to indoors) in Kérou. This low biting behaviour of An. nili group inside the dwellings had also been reported in Nigeria [39]. Kérou being a savanna area, this behaviour of An. nili group confirms the results of other authors who have shown that savanna populations of An. nili group are more exophagic and exophilic [40, 41].

In Kérou, each inhabitant receives 5 bites of An. nili group per night. This biting rate was significantly higher than the one of An. funestus group and not negligible for just four nights of collection. This reflects the presence of breeding sites favorable to the development of this species in the area. In fact, Kérou is close to several rivers, and the water level of these rivers is kept high for a good part of the year by the rain. This flow of water contributes to the development of several permanent mosquito breeding sites. This could probably explain the high density of the populations of the An. nili group in Kérou. As a result, in an area with multiple vectors, variation in ecological, spatial and temporal parameters directly influences the relative role of each species in malaria transmission [42]. The biting rate of An. gambiae s.l. was very high in Kérou (26.19 bites/human/night) and in Péhunco (4 bites/human/night) compared to the other species and allows this vector to maintain the transmission of malaria in both districts.

Another important aspect of the current study is the involvement of An. nili group in malaria transmission in Benin. Indeed, the SI of An. nili group was 1.25% in Kérou. This index is similar to that recorded by Carnevale et al. [35] in Cameroon and lower than the one of Elissa et al. [43] in Gabon and Adja et al. [31] in Côte d’Ivoire. The sporozoite indexes of An. gambiae s.l. in Kérou (1.7%) and Péhunco (0.9%) were lower than those reported by previous studies in different areas of Benin [16, 33, 37]. This could be due to the low number of tested mosquitoes in the short collection period covered by the current study as compared to previous records which considered a longer time period.

This study also showed malaria transmission by different mosquito species in the Kérou district. An. gambiae s.l. and An. nili group share in the indoors and outdoors malaria transmission. In An. funestus group where EIR has been observed indoors, no definitive conclusion can be drawn as to its participation in outdoor transmission since the collection period in this study is short. As previously found in several sites in Benin [13-18], An. gambiae s.l. also remains the primary vector of malaria transmission in Kérou (13.05 infected bites/human/month), followed by An. nili group (1.87 infected bites/human/month) and An. funestus group (0.94 bites/human/month).

The EIR by An. gambiae s.l. in Kérou was higher than those reported in a longitudinal study by Salako et al. [37] in similar bioecological areas (Kandi and Gogounou) in the department of Alibori, Benin. In Péhunco, each inhabitant receives 1.02 infected bites of An. gambiae s.l. per month. The high transmission by An. gambiae s.l. in Kérou compared to both An. funestus group and An. nili group may be due to its significantly higher physiological age compared to the one of the other mosquito species (p < 0.05). The small number of An. funestus group collected in each district could explain its low level of malaria transmission. Findings of this study shows that no specimen of An. nili group and An. funestus group was collected indoors after PSC. This could be due to the low sampling of mosquitoes performed during our survey. An. gambiae s.l. which was the most frequent vector in the two districts takes mostly its blood meal on humans, thus confirming its anthropophagic nature [33].

Regarding the presence of resistance mechanisms in An. gambiae s.l., the L1014F Kdr mutation was found at very high frequencies in its two detected sibling species (An. gambiae and An. coluzzii) in the two districts. Contrary to the works of Gnanguenon et al. [44], Yahouédo et al. [45] and Akogbeto et al. [33] in some locations of southern and northern Benin, no significant difference was noted between the allelic frequencies of the L1014F kdr mutation of An. gambiae and An. coluzzii in Kérou and Péhunco. This result corroborates those of Salako et al. [46] who also found no significant difference between the L1014F kdr frequencies of An. gambiae and An. coluzzii in Djougou, Ségbana and Copargo, northern Benin. The trend was the same for the relatively low allelic frequencies of the G119S ace-1 mutation of An. gambiae and An. coluzzii of the two study districts. However, the highest allelic frequencies of the G119S Ace-1 mutation was recorded in An. gambiae in each zone with values ranging from 5 to 7.3%. In An. coluzzii, this frequency varied from 0% in Péhunco to 5% in Kérou.

Conclusion

In the present study, An. gambiae s.l., An. funestus group and An. nili group were the three most important vectors that transmit malaria in the Kérou district. This is the first report on the contribution of the An. nili group as a secondary vector of malaria transmission in Benin. This suggests broader studies involving other districts to determine if other Anopheles species also contribute to malaria transmission. This will help a better orientation of vector control interventions.