| Literature DB >> 31011186 |
Eleni P Mimitou1, Anthony Cheng2,3, Antonino Montalbano4,5, Stephanie Hao1, Marlon Stoeckius1, Mateusz Legut4,5, Timothy Roush4,5, Alberto Herrera6, Efthymia Papalexi4,5, Zhengqing Ouyang2,3,7, Rahul Satija4,5, Neville E Sanjana4,5, Sergei B Koralov6, Peter Smibert8.
Abstract
Multimodal single-cell assays provide high-resolution snapshots of complex cell populations, but are mostly limited to transcriptome plus an additional modality. Here, we describe expanded CRISPR-compatible cellular indexing of transcriptomes and epitopes by sequencing (ECCITE-seq) for the high-throughput characterization of at least five modalities of information from each single cell. We demonstrate application of ECCITE-seq to multimodal CRISPR screens with robust direct single-guide RNA capture and to clonotype-aware multimodal phenotyping of cancer samples.Entities:
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Year: 2019 PMID： 31011186 PMCID： PMC6557128 DOI： 10.1038/s41592-019-0392-0
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547