| Literature DB >> 31787378 |
Ian Setliff1, Andrea R Shiakolas2, Kelsey A Pilewski2, Amyn A Murji2, Rutendo E Mapengo3, Katarzyna Janowska4, Simone Richardson5, Charissa Oosthuysen5, Nagarajan Raju2, Larance Ronsard6, Masaru Kanekiyo7, Juliana S Qin8, Kevin J Kramer2, Allison R Greenplate8, Wyatt J McDonnell9, Barney S Graham7, Mark Connors10, Daniel Lingwood6, Priyamvada Acharya11, Lynn Morris12, Ivelin S Georgiev13.
Abstract
B cell receptor (BCR) sequencing is a powerful tool for interrogating immune responses to infection and vaccination, but it provides limited information about the antigen specificity of the sequenced BCRs. Here, we present LIBRA-seq (linking B cell receptor to antigen specificity through sequencing), a technology for high-throughput mapping of paired heavy- and light-chain BCR sequences to their cognate antigen specificities. B cells are mixed with a panel of DNA-barcoded antigens so that both the antigen barcode(s) and BCR sequence are recovered via single-cell next-generation sequencing. Using LIBRA-seq, we mapped the antigen specificity of thousands of B cells from two HIV-infected subjects. The predicted specificities were confirmed for a number of HIV- and influenza-specific antibodies, including known and novel broadly neutralizing antibodies. LIBRA-seq will be an integral tool for antibody discovery and vaccine development efforts against a wide range of antigen targets.Entities:
Keywords: B cells; HIV; antibodies; antibody discovery; antibody repertoire; broadly neutralizing antibody; influenza; single cell immunology; systems immunology
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Year: 2019 PMID： 31787378 PMCID： PMC7158953 DOI： 10.1016/j.cell.2019.11.003
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582