| Literature DB >> 12938510 |
J Zhou1, R Petracca.
Abstract
Three diphtheria toxin (DT) mutants CRM-197, DT-del (148) and DT-E148S-K516A-F530A were cloned in B. Subtilis plasmid PSM604 under the subtilisin signal sequence. The expression was effective in both SMS300 and SMS118, but higher yield of 7.1 mg/L was observed in SMS300 compared with 2.1 mg/L in SMS118. Western blot showed that the recombinant protein could be effectively secreted into the culture medium as a 58 ku peptide, and could be degraded into two peptides of 37 ku and 21 ku.Entities:
Mesh：
Substances：
Year: 1999 PMID： 12938510 DOI： 10.1007/bf02886955
Source DB: PubMed Journal: J Tongji Med Univ ISSN： 0257-716X