Normal Liver Stiffness Values in Children: A Comparison of Three Different Elastography Methods.

OBJECTIVES: Noninvasive tests for the evaluation of liver fibrosis are particularly helpful in children to avoid general anesthesia and potential complications of invasive tests. We aimed to establish reference values for 2 different elastography methods in a head-to-head comparison for children and adolescents 4 to 17 years, using transient elastography as common reference in a subset.
METHODS: A total of 243 healthy participants aged 4 to 17 years were examined by a single observer with a full liver B-mode scan before elastography, following a minimum of 3 hours fasting. Liver stiffness measurements (LSMs) using 2-dimensional shear wave elastography (2D-SWE, GE Logiq E9) and point shear wave elastography (pSWE, Samsung RS80A with Prestige) were performed in all participants, and compared to transient elastography (TE, FibroScan) in a subset (n = 87). Interobserver agreement was evaluated in 50 children aged 4 to 17 years.
RESULTS: Valid measurements were obtained in 242 of 243 (99.6%) subjects for 2D-SWE, 238 of 243 (97.9%) for pSWE, and in 83 of 87 (95.4%) for TE. Median liver stiffness overall was 3.3 (interquartile range [IQR] 2.7-4.3), 4.1 (IQR 3.6-4.7), and 4.1 kPa (IQR 3.5-4.6) for 2D-SWE, pSWE, and TE, respectively. Intraclass correlation coefficients between observers were 0.84 and 0.83 for 2D-SWE and pSWE, respectively. LSM values were significantly lower for 2D-SWE compared to pSWE and TE, and increased with advancing age. Higher LSM values in males were observed in adolescents.
CONCLUSIONS: All methods showed excellent feasibility. 2D-SWE showed significantly lower LSM values than pSWE and TE, and lower failure rate compared to TE. Our results further indicate an age and sex effect on LSM values.

What Is Known

Liver fibrosis is difficult to assess without a liver biopsy, which in children often require general anesthesia.

Liver elastography is increasingly used as a noninvasive surrogate marker of liver fibrosis.

Different ultrasound platforms yield different values.

What Is New

This is the first publication of reference values for both point shear wave elastography and 2-dimensional shear wave elastography in healthy children, with comparison to transient elastography.

Two-dimensional shear wave elastography yielded significantly lower liver stiffness measurements than point shear wave elastography and transient elastography.

Liver stiffness measurements increased with age and were higher in male than female adolescents.

Ultrasound elastography is increasingly used to estimate liver fibrosis in children and adolescents, and has been reported in pediatric populations with mixed liver diseases (1–3) and in more homogenous populations (eg, biliary atresia) (4–8). The technique is continuously improving, and a sensitivity and specificity of 81% and 91%, respectively, was recently reported for liver fibrosis in children (2). The criterion standard for liver fibrosis evaluation is the liver biopsy, challenged due to its invasive nature, the need for general anesthesia and the potential of sampling errors and clinical complications (9). Ultrasound elastography is noninvasive and allows evaluation of the entire organ, with minimal discomfort for the patient, albeit lacking the liver biopsy's ability to assess the etiology. Awareness of factors other than fibrosis which can influence liver stiffness is necessary (10). Transient elastography (TE) was introduced first and is established in several clinical settings. However, recent years have seen the introduction of alternative platforms allowing simultaneous B-mode imaging, including point shear wave (pSWE) and 2-dimensional shear wave elastography (2D-SWE), based on similar principles, with similar recommendations regarding use and application (10). Liver stiffness measurements (LSMs) have been demonstrated to vary between different elastography methods in both children (2) and adults (11,12); thus, normal values should be defined for each platform. There are currently no publications comparing LSM across platforms in healthy children. We aimed to establish and compare reference values for pSWE and 2D-SWE, with head-to-head comparison to TE in a subset.

METHODS

Subjects

The study was performed at Haukeland University Hospital in Bergen, Norway from September 2017 through January 2018. Participants were recruited through hospital employees, local schools, and social media. Exclusion criteria were a history of liver disease or chronic disease which could affect the liver. Informed written consent was obtained. A total of 246 children aged 4 to 17 years were recruited and grouped into 4 predefined age categories: 4 to 7; 8 to 11; 12 to 14; and 15 to 17 years. Two hundred thirty (94.7%) had Caucasian parents. The medical history was recorded, including the use of alcohol or nicotine. All were evaluated clinically by a pediatrician (A.B.M.) with >10 years’ experience. Height, weight, waist circumference, and body mass index (BMI) were recorded and converted into z scores by the means of the Norwegian growth references (13,14). Weight classes were defined using International Obesity Task Force (IOTF) definitions (15). Blood tests were not performed. Participants classified as obese (n = 5) were included. Subjects with B-mode signs of steatosis or splenomegaly (n = 2), or fasting <3 hours (n = 1), were excluded, leaving 243 (108 boys, 44.4%) for further analyses.

B-mode Ultrasound Evaluation

B-mode ultrasound was performed after a standardized protocol with evaluation of the liver, gall bladder, spleen, and kidneys before elastography measurements, using Samsung RS80A with Prestige, with a convex 1 to 7 MHz probe. Skin to liver capsule distance was recorded. Examinations were conducted by a single operator (A.B.M.) with >2 years’ experience in abdominal ultrasound.

Liver Stiffness Measurements

Ultrasound elastography measurements were performed in a supine position with the right arm maximally abducted, after ≥3 hours of fasting. Participants were examined with both GE Logiq E9 2D-SWE, using a C1–6 probe (GE Healthcare, Milwaukee, WI) and Samsung RS80A with Prestige pSWE, using a CA1–7A probe (Samsung Medison Co, Ltd, Seoul, Korea). SWE measurements were obtained in the right liver lobe applying minimal pressure through an intercostal space, perpendicular to the capsule, avoiding large liver vessels, bile ducts, and rib shadowing. Acquisitions were made during mid-expiratory breath hold if possible, otherwise during calm expiration. LSM values are expressed in meters per second (m/s) or kilopascals (kPa), the latter being calculated using the equation kPa = 3(ms−1)2. We performed 10 valid acquisitions, and reported median values expressed in kPa for all systems. Every acquisition and mean, median, and interquartile range/median (IQR/M%; measure of dispersion) was recorded. A valid LSM value was defined as the median of 10 valid acquisitions with an IQR/M% ≤30%. For Samsung, values in m/s, average measurement depth and reliability measurement index, were automatically recorded, with a fixed region of interest (ROI) of 1 × 1.5 cm. For GE (2D-SWE), the ROI was a fixed circle with a diameter of 1 cm. ROIs were placed 2 to 5 cm from the liver capsule. In a subset of 50 subjects, 2 observers (A.B.M. and A.M.) both obtained data using GE and Samsung for interobserver reliability analysis. Both investigators had >2 years of experience in liver elastography. In a subset of patients ≥8 years (n = 87), TE using FibroScan (M-probe) incorporated in a GE S8 (GE Healthcare) was performed, reporting LSM results in kPa. For TE, the additional criterion of success rate ≥60% was adopted. The M-probe has been used extensively in children and adults with thorax perimeter under the recommended 75 cm, having been shown to affect feasibility only slightly (16); furthermore, we did not have access to the smaller S probe. The XL probe is known to yield lower values, and none of the subjects had skin to capsule distance ≥2.5 cm, for which an XL probe is warranted.

Controlled Attenuation Parameter

Fat deposits in hepatocytes affect ultrasound propagation, increasing the attenuation. Controlled attenuation parameter (CAP) evaluates the ultrasonic attenuation in the liver at 3.5 MHz at depth 25 to 65 mm using FibroScan, and represents a noninvasive assessment of liver steatosis (17). CAP values in dB/m were reported as the median of 10 acquisitions for all subjects evaluated by TE.

Statistical Analysis

For all analyses, SPSS version 25 (SPSS Inc, 2016, Armonk, NY) was used. Variables were tested for normality, and data were presented as mean (standard deviation [SD]) or median (range), as appropriate. When establishing age-specific reference values, mean ± 1.96 SD was used. For comparison of groups, standard paired T test, Wilcoxon signed rank test, or Pearson Chi-Square test were used as appropriate. Correlations were tested by Pearson correlation coefficient. Intraclass correlation coefficients (ICCs) were calculated to present interobserver reliability. Limits of agreement were assessed to reveal differences between platforms and observers. P values <0.05 were considered significant.

Ethical Aspects

The protocol was in accordance with the Declaration of Helsinki and approved by the Regional Committee on Medical and Health Research Ethics of Western Norway (2017/290/REK Vest).

RESULTS

The characteristics of the 243 subjects included are displayed in Table 1. Valid measurements were obtained in 242 of 243 (99.6%) for 2D-SWE, 238 of 243 (97.9%) for pSWE, and 83 of 87 (95.4%) for TE. TE feasibility was significantly lower than 2D-SWE feasibility (P = 0.03), but not different from pSWE (P = 0.47); nonfeasibility was most often due to wide dispersion (IQR/M% >30%) reflecting insufficient reliability. Median (range) IQR/M values were 10.1 (1.4–40), 13.9 (1.3–44), and 12.0 (3–44) for 2D-SWE, pSWE and TE, respectively. Among the excluded was an extreme outlier of 395% for pSWE, with corresponding reliability measurement index of 0.1 (the producer recommends ≥0.4). Two hundred thirty-seven subjects (97.5%) showed valid results for both 2D-SWE and pSWE; 81 (93.1%) for all 3 platforms.

TABLE 1

Baseline characteristics for all children and adolescents included for liver elastography in a study using ultrasound 2-dimensional shear wave elastography, point shear wave elastography, and transient elastography

	Total panel	4–7 y	8–11 y	12–14 y	15–17 y
Number	243	59	64	59	61
Males, number (%)	108 (44.4%)	31 (52.5%)	26 (40.6%)	30 (50.8%)	21 (34.4%)
Age, y, median (range)	11.7 (4.1–17.9)	6.3 (4.1–7.9)	10.0 (8.1–11.8)	13.4 (12–15.0)	17.1 (15–17.9)
Waist circumference, cm, median (range)	60.0 (45–98)	52 (45–59)	58 (50–75)	64 (51–85)	70 (60.5–98)
Weight, kg, median (range)	40.8 (13.7–105)	22.0 (13.7–32.7)	33.5 (22.2–61.7)	47.5 (28.7–80.7)	63.2 (41.6–105)
Body mass index (BMI)	17.6 (12–30.6)	15.5 (12–18.9)	17.2 (14–25.7)	17.9 (13.4–28.9)	21.5 (17.5–30.6)
Overweight or obese according to IOTF, n (%)	27 (11.1)	3 (5.1)	6 (9.4)	5 (8.5)	13 (21.3)
Mid-expiratory breath hold during measurement	195 (80.2%	11/59 (18.6%)	64/64 (100%)	59/59 (100%)	61/61 (100%)
Skin-to-capsule*, cm	1.11 (0.70–2.64)	0.9 (0.7–1.24)	1.03 (0.72–1.77)	1.17 (0.85–2.14)	1.41 (1.05–2.64)
Alcohol consumption last 72 h, n (%)	7 (2.9%)	0 (0%)	0 (0%)	0 (0%)	7 (11.5%)

IOTF = International Obesity Task Force.

*Distance from skin to liver capsule in centimeters.

Median Liver Stiffness and Measurement Variability for 2-Dimensional Shear Wave Elastography, Point Shear Wave Elastography, and Transient Elastography

LSM values for 2D-SWE were significantly lower compared with pSWE or TE (median LSM 3.3, 4.1, and 4.1 kPa, respectively; P < 0.001), with no difference between pSWE and TE (P = 0.65) (Table 2). Moreover, the slope of LSM values was steeper for 2D-SWE compared to pSWE, with lower values for 2D-SWE compared to corresponding pSWE values for LSM <4 kPa by 2D-SWE, but higher values for 2D-SWE compared to corresponding pSWE values for LSM >5 kPa by 2D-SWE (Supplementary Fig. 1, Supplemental Digital Content). In the total panel, 2D-SWE and pSWE showed moderate correlation (rho = 0.51, P < 0.001).

TABLE 2

Liver stiffness measurements values by 2-dimensional shear wave elastography and point shear wave elastography for children aged 4 to 17 years

	GE Logiq E9 (2D-SWE)			Samsung RS80A (pSWE)
	Mean value, kPa ± SD (97.5 percentile)	Range, kPa	Calculated mean value in m/s (97.5 percentile)	Mean value, kPa ± SD (97.5 percentile)	Range, kPa	Mean value, m/s ± SD (97.5 percentile)
4–7 y	2.87 ± 0.56 (3.96)	2.0–4.7	0.98 m/s (1.15)	3.93 ± 0.56 (5.03)	2.8–5.2	1.14 ± 0.08(1.30)
8–11 y	3.45 ± 1.03 (5.47)	2.1–6.5	1.07 m/s (1.35)	4.17 ± 0.74 (5.61)	3.1–6.4	1.17 ± 0.10(1.37)
12–14 y	3.83 ± 1.27 (6.32)	2.0–7.7	1.13 m/s (1.45)	4.65 ± 0.83 (6.27)	3.1–6.5	1.24 ± 0.11(1.46)
15–17 y	3.96 ± 1.06 (6.03)	2.0–7.0	1.15 m/s (1.42)	4.23 ± 0.91 (6.02)	2.9–7.1	1.18 ± 0.12(1.42)

Liver stiffness measurement (LSM) values represent the mean of LSM results from children in each age group. Individual LSM results are based on median values from 10 valid acquisitions.

2D-SWE = 2-dimensional shear wave elastography; pSWE = point shear wave elastography; SD = standard deviation.

The coefficient of variation (CV) between the 10 serial acquisitions forming a single LSM value, was low for all systems, ranging from 0.03 to 1.54, across all age groups (0.03–0.21 for pSWE, 0.03–0.24 for 2D-SWE, and 0.03–1.54 for TE). The highest CV for TE was due to a single high acquisition in 1 subject, which if excluded would have yielded a range of 0.03 to 0.29. The CV was slightly lower for 2D-SWE compared to pSWE (P = 0.009) and TE (P = 0.006), with similar values for pSWE and TE (P = 0.12).

Interobserver Evaluation

Interobserver reliability was evaluated in participants from all age groups with valid LSM for pSWE (n = 48) and 2D-SWE (n = 50). There were no significant differences between observers for pSWE (medians 4.10 kPa [IQR 3.6–4.9] vs 4.15 kPa [3.4–4.6]) or 2D-SWE (medians 3.55 kPa [IQR 2.8–4.3] vs 3.55 kPa [2.8–4.3]). ICCs between observers were 0.83 (95% confidence interval 0.7–0.91) and 0.84 (95% confidence interval 0.71–0.91) for pSWE and 2D-SWE, respectively, with no systematic differences between observers (Suppl. Figs. 2A and 2B, Supplemental Digital Content). The average difference (95% limits of agreement) was +2.1% (−26.2%–30.4%) and −0.1% (−35.9%–35.7%) for pSWE and 2D-SWE, respectively. A small number of subjects showed a discrepancy >1 kPa between observers, 4 of 48 (8.3%) and 5 of 50 (10%) for pSWE and 2D-SWE, respectively. Only 1 of 50 (2%) of subjects showed a difference >1.6 kPa using 2D-SWE, and none using pSWE.

Difference in Liver Elasticity by Age and Sex

For 2D-SWE, LSM was associated with age (rho = 0.421, P < 0.001) and increased steadily until 12 to 17 years, with no significant difference between the 2 older age groups (Table 2, Fig. 1).

FIGURE 1

Liver stiffness measurements by age groups and elastography systems: point shear wave elastography (pSWE, all age groups, n = 238), 2-dimensional shear wave elastography (2D-SWE, all age groups, n = 243), and transient elastography (TE, age groups 2–4, n = 83). For pSWE: group 1 significantly lower liver stiffness measurements (LSM) values than groups 2 to 4 and group 3 significantly higher than group 2 and 4. For 2D-SWE: LSM values rising significantly from group 1 to group 2 and from group 2 to group 4. For TE: LSM values in group 3 and 4 were significantly higher than that in group 2.

For pSWE, LSM showed a weak association with age (rho = 0.146, P = 0.02) for the 3 youngest age groups (Table 2, Fig. 1). LSM age 8 to 11 was significantly lower than LSM age 12 to 14 years (P = 0.001), and significantly higher than LSM age 4 to 7 (P = 0.04), whereas LSM 15 to 17 years was significantly lower than 12 to 14 years (P = 0.002).

Overall, boys showed higher values compared to girls for pSWE. In subgroup analyses, sex difference was found in adolescence only: isolating ages 12 to 17 years, we found significantly higher LSM values in boys across all platforms, with mean values 4.27 versus 3.62 kPa (P = 0.002), 4.68 versus 4.27 kPa (P = 0.01), and 4.68 versus 4.13 kPa (P = 0.02) for 2D-SWE, pSWE, and TE, respectively (Fig. 2).

FIGURE 2

Liver stiffness measurements in boys (blue) and girls (red) aged 12 to 17 years as assessed by point shear wave elastography (pSWE), 2-dimensional shear wave elastography (2D-SWE), and transient elastography (TE). The figure shows liver stiffness as assessed by pSWE (Samsung RS80A with Prestige; n = 117), 2D-SWE (GE Logiq E9, n = 120), and TE (FibroScan, n = 60), respectively. Boxes represent the central 50% of the values, with the median value given as a horizontal line, and whiskers representing minimum and maximum, excluding outliers (small circles). Overall liver stiffness measurements (LSM) values are significantly lower in girls for all platforms: pSWE (P = 0.01), 2D-SWE (P = 0.002), and TE (P = 0.02).

Obesity-related Characteristics, Liver Stiffness Measurement and Controlled Attenuation Parameter

BMI z scores or IOTF weight classes were not distributed evenly across age groups, and analyses and comparisons were performed within age groups. Overweight or obese groups yielded no significant differences for pSWE, when compared with the nonoverweight, but for 2D-SWE there was significantly higher LSM values for the overweight aged 8 to 11 (3.35 vs 4.45 kPa, P = 0.01) and 15 to 17 years (3.78 vs 4.64 kPa, P = 0.008), but not for ages 4 to 7 or 12 to 14 years. TE showed lower LSM values in the overweight aged 15 to 17 years (4.52 vs 3.4 kPa, P = 0.03), but not for 12 to 14 years.

The skin to capsule distance correlated with LSM for 2D-SWE (rho = 0.355, P < 0.001), but not with LSM for pSWE or TE (P > 0.2). In multiple linear regression (correcting for anthropometric measurements and age) skin to capsule distance was not independently associated with LSM. Skin to capsule distance was moderately correlated to anthropometric measures (rho = 0.516, 0.471 and 0.456 for BMI, waist, and weight z scores, respectively; all P values <0.001).

CAP values (estimating liver steatosis) were normally distributed, with an overall mean value 191.9 dB/m (SD 38.1), range 100 to 296. Six subjects displayed values above the proposed cut-off of 249 dB (18); 5 of these had normal weight. Using linear regression, CAP was associated with BMI z score (P = 0.005), but not with LSM by pSWE or 2D-SWE, age, or sex. CAP was shown to rise steadily already from BMI z score 0 and was significantly higher when comparing children with BMI z score ≥0 and BMI z score <0 (205 vs 180 dB, P = 0.002).

Quality Indicators and Associations With Body Mass Index and Skin-to-capsule Distance

IQR/M% is a frequently used quality indicator in LSM, with IQR/M% <30% commonly used as cutoff for a valid result in kPa, reflecting limited spread in the values of acquisitions that collectively make up 1 LSM value. We found no correlation between IQR/M% and a high or low LSM value for pSWE and TE, and not for 2D-SWE after adjusting for age. As expected, IQR/M% values were approximately twice as high for measurements in kPa compared to m/s in the same individuals.

DISCUSSION

We report on the normal liver stiffness in healthy children aged 4 to 17 years. To our knowledge, this is the first head-to-head comparison between 2D-SWE and pSWE, with TE as common reference in a subset. Feasibility was excellent for all systems with failure rates of 0.4%, 2.1%, and 4.6% for 2D-SWE, pSWE, and TE, respectively, showing slightly but significantly superior feasibility for 2D-SWE compared to TE, in line with previous studies in adults. Failure was due to a high IQR/M >30% (n = 9) or success rate <60% (n = 1; TE). Feasibility was good using the M-probe for TE in children with a thorax perimeter <75 cm. We have demonstrated a low variation (CV) for the different platforms, and a good ICC between observers, but the average interobserver difference shown as 95% limits of agreement (−26.2%–30.4% and −35.9%–35.7% for pSWE and 2D-SWE, respectively) should be noted.

In the total panel, median liver stiffness was 3.3 kPa (1.05 m/s; range 2.0–7.7 kPa), 4.1 kPa (1.17 m/s; range 2.8–7.1 kPa), and 4.1 kPa (1.17 m/s; range 2.4–11.2 kPa) for 2D-SWE, pSWE, and TE, respectively. This is in line with previously published values for healthy children, reporting mean LSM of 4.6 kPa (19–24) and 1.12 m/s (25–31) by TE and acoustic radiation force impulse (ARFI), respectively (medians of reported values) (19–31).

Overall, we found significantly lower values using 2D-SWE compared to pSWE and TE (P < 0.001), whereas pSWE and TE showed similar LSM values (P = 0.65). This contrasts with reports in healthy adults, showing higher LSM values for 2D-SWE compared to pSWE and TE (11,12). Our results, however, indicate that the slope of the curve for LSM is steeper for 2D-SWE compared to pSWE; thus, in subjects with LSM >4 kPa the mean LSM by 2D-SWE was higher compared to LSM by pSWE (Suppl. Fig. 1, Supplemental Digital Content). Several publications comparing different platforms in adults (32–34) and phantoms (35,36) have shown similar relationships, but with these differences only evident outside the normal range. As higher LSMs are reported in healthy adults compared to children, the finding of higher values when using 2D-SWE compared to pSWE in adults (11), fits with our results. We can only speculate that 2D-SWE may yield higher LSM values than pSWE in children with significant liver fibrosis. We have not found other studies comparing the methods used herein. In a small study, children with heterogeneous chronic liver diseases were investigated with 2D-SWE, ARFI, and TE, but LSM values were not compared across platforms in the individual subjects (37) as in our head-to-head comparison.

Our findings strongly indicate that liver stiffness increases during childhood and adolescence. Previous studies on children investigating single elastography platforms (TE, 2D-SWE, and ARFI, respectively) are discordant regarding the association of age with LSM; some report increasing values with age (19,21,23,25,31,38), some do not (20,22,26–28,39,40). We found higher values in boys compared to girls, during adolescence. This is in line with several adult studies, reporting higher values in males for 2D-SWE (12,41), pSWE (42), and TE (43,44); however, some describe no significant sex difference for ARFI (45) and pSWE (12). Three pediatric studies have similarly demonstrated higher LSM values in boys compared with girls (19,26,38), one of which only in older children, whereas 6 others did not find such a difference (21,22,25,27,31,39).

We aimed to describe liver stiffness in a healthy pediatric population, and therefore excluded subjects with B-mode signs of steatosis. Nevertheless, a minority of the subjects were overweight (n = 24) or obese (n = 3) as defined by IOTF. Although we found significant differences in LSM between the overweight and the normal weight in 2 age groups for 2D-SWE and 1 age group for TE, our results should be interpreted with caution due to a low number of overweight and obese in each age group. A recent publication (46) using a different pSWE method, studied the effect of BMI on LSM values, and found higher LSM values in the obese. The same study found no correlation between LSM value and BMI within the nonobese cohort, which included overweight children.

EFSUMB guidelines (10) describe the use of IQR/M% ≤30% as a quality indicator for pSWE and 2D-SWE, mimicking the FibroScan criterion, but do not mention that this parameter will change significantly based on the measurement unit. Comparing IQR/M% for m/s and kPa in our material, we illustrated a linear relationship, with the latter twice as high, demonstrating that the IQR/M% cut-off applies for kPa only (Suppl. Fig. 3, Supplemental Digital Content).

The present article does not allow conclusions regarding the superiority of any platform. In our experience, however, the availability of simultaneous B-mode imaging adds important value, allowing a full investigation in 1 session and sometimes a better assessment of factors influencing results. The learning curve for pSWE seems steeper and may thus be better for training users without extensive experience in B-mode imaging, whereas 2D-SWE may seem less prone to failed measurements in experienced users.

The study only includes healthy children and adolescents, and the findings are not automatically applicable in pediatric liver patients. Our findings and differences found have to some extent been described in adult liver patients, and this should be further investigated in children and adolescents with chronic liver diseases. Although there is little suspicion of liver diseases in our cohort, a biochemical evaluation of the participants would have strengthened our results.

CONCLUSIONS

For the first time we have described normal values for liver stiffness in healthy children using ultrasound elastography by pSWE and 2D-SWE in a head-to-head comparison, and compared to TE as reference in a subset. We demonstrated high feasibility in children for all methods with best results for the combination of ultrasound and elastography, but interobserver variability demonstrates the need of standardization of methods. Our results indicate different reference values depending on age and sex with increasing liver stiffness with age and particularly higher LSM values in boys during adolescence. This must be considered when using the methods in clinical practice. Further studies exploring elastography methods head-to-head in pediatric liver patients may further enhance our understanding of the differences between methods and the clinical utility of this tool.