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Literature DB >> 3056718

A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5' cleavage site.

Abstract

We analyzed the effects of suppressor mutations in the U1 snRNA (SNR19) gene from Saccharomyces cerevisiae on the splicing of mutant pre-mRNA substrates. The results indicate that pairing between U1 snRNA and the highly conserved position 5 (GTATGT) of the intron occurs early in spliceosome assembly in vitro. This pairing is important for efficient splicing both in vitro and in vivo. However, pairing at position 5 does not appear to influence 5' splice site selection in vivo, indicating that the previously described U1 snRNA:5' splice junction base pairing interaction is not sufficient to define the 5' cleavage site.

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Year: 1988 PMID： 3056718 PMCID： PMC457124 DOI： 10.1002/j.1460-2075.1988.tb03101.x

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

34 in total

1. In vitro splicing of mRNA precursors: 5' cleavage site can be predicted from the interaction between the 5' splice region and the 5' terminus of U1 snRNA.

Authors: S Weber; M Aebi
Journal: Nucleic Acids Res Date: 1988-01-25 Impact factor: 16.971

2. A point mutation in the conserved hexanucleotide at a yeast 5' splice junction uncouples recognition, cleavage, and ligation.

Authors: R Parker; C Guthrie
Journal: Cell Date: 1985-05 Impact factor: 41.582

3. Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences.

Authors: D Frendewey; W Keller
Journal: Cell Date: 1985-08 Impact factor: 41.582

4. Gel electrophoretic isolation of splicing complexes containing U1 small nuclear ribonucleoprotein particles.

Authors: M Zillmann; M L Zapp; S M Berget
Journal: Mol Cell Biol Date: 1988-02 Impact factor: 4.272

5. mRNA splicing efficiency in yeast and the contribution of nonconserved sequences.

Authors: C W Pikielny; M Rosbash
Journal: Cell Date: 1985-05 Impact factor: 41.582

6. One-step gene disruption in yeast.

Authors: R J Rothstein
Journal: Methods Enzymol Date: 1983 Impact factor: 1.600

7. A single-base change at a splice site in a beta 0-thalassemic gene causes abnormal RNA splicing.

Authors: R Treisman; N J Proudfoot; M Shander; T Maniatis
Journal: Cell Date: 1982-07 Impact factor: 41.582

8. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

Authors: K Struhl; D T Stinchcomb; S Scherer; R W Davis
Journal: Proc Natl Acad Sci U S A Date: 1979-03 Impact factor: 11.205

9. Transformation of intact yeast cells treated with alkali cations.

Authors: H Ito; Y Fukuda; K Murata; A Kimura
Journal: J Bacteriol Date: 1983-01 Impact factor: 3.490

10. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.

Authors: J D Boeke; F LaCroute; G R Fink
Journal: Mol Gen Genet Date: 1984

170 in total

1. Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction.

Authors: G Ast; T Pavelitz; A M Weiner
Journal: Nucleic Acids Res Date: 2001-04-15 Impact factor: 16.971

10. Sequences homologous to 5' splice sites are required for the inhibitory activity of papillomavirus late 3' untranslated regions.

Authors: P A Furth; W T Choe; J H Rex; J C Byrne; C C Baker
Journal: Mol Cell Biol Date: 1994-08 Impact factor: 4.272

A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5' cleavage site.

1. In vitro splicing of mRNA precursors: 5' cleavage site can be predicted from the interaction between the 5' splice region and the 5' terminus of U1 snRNA.

2. A point mutation in the conserved hexanucleotide at a yeast 5' splice junction uncouples recognition, cleavage, and ligation.

3. Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences.

4. Gel electrophoretic isolation of splicing complexes containing U1 small nuclear ribonucleoprotein particles.

5. mRNA splicing efficiency in yeast and the contribution of nonconserved sequences.

6. One-step gene disruption in yeast.

7. A single-base change at a splice site in a beta 0-thalassemic gene causes abnormal RNA splicing.

8. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

9. Transformation of intact yeast cells treated with alkali cations.

10. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.

1. Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction.

2. Yeast U1 snRNP-pre-mRNA complex formation without U1snRNA-pre-mRNA base pairing.

3. Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex.

4. Interaction of the U1 snRNP with nonconserved intronic sequences affects 5' splice site selection.

5. ATP-dependent interaction of yeast U5 snRNA loop 1 with the 5' splice site.

6. Activation of a cryptic 5' splice site by U1 snRNA.

7. Dhr1p, a putative DEAH-box RNA helicase, is associated with the box C+D snoRNP U3.

8. The U1 snRNP base pairs with the 5' splice site within a penta-snRNP complex.

9. Cotranscriptional recruitment of the U1 snRNP to intron-containing genes in yeast.

10. Sequences homologous to 5' splice sites are required for the inhibitory activity of papillomavirus late 3' untranslated regions.