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Literature DB >> 6310324

One-step gene disruption in yeast.

Abstract

The one-step gene disruption techniques described here are versatile in that a disruption can be made simply by the appropriate cloning experiment. The resultant chromosomal insertion is nonreverting and contains a genetically linked marker. Detailed knowledge of the restriction map of a fragment is not necessary. It is even possible to "probe" a fragment that is unmapped for genetic functions by constructing a series of insertions and testing each one for its phenotype.

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Year: 1983 PMID： 6310324 DOI： 10.1016/0076-6879(83)01015-0

Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600

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Cited

1500 in total

1. Stress tolerance in doughs of Saccharomyces cerevisiae trehalase mutants derived from commercial Baker's yeast.

Authors: J Shima; A Hino; C Yamada-Iyo; Y Suzuki; R Nakajima; H Watanabe; K Mori; H Takano
Journal: Appl Environ Microbiol Date: 1999-07 Impact factor: 4.792

2. The mechanism of mammalian gene replacement is consistent with the formation of long regions of heteroduplex DNA associated with two crossing-over events.

Authors: J Li; L R Read; M D Baker
Journal: Mol Cell Biol Date: 2001-01 Impact factor: 4.272

3. A fission yeast gene, him1(+)/dfp1(+), encoding a regulatory subunit for Hsk1 kinase, plays essential roles in S-phase initiation as well as in S-phase checkpoint control and recovery from DNA damage.

Authors: T Takeda; K Ogino; E Matsui; M K Cho; H Kumagai; T Miyake; K Arai; H Masai
Journal: Mol Cell Biol Date: 1999-08 Impact factor: 4.272

4. Pleiotropic alterations in lipid metabolism in yeast sac1 mutants: relationship to "bypass Sec14p" and inositol auxotrophy.

Authors: M P Rivas; B G Kearns; Z Xie; S Guo; M C Sekar; K Hosaka; S Kagiwada; J D York; V A Bankaitis
Journal: Mol Biol Cell Date: 1999-07 Impact factor: 4.138

5. Proper metaphase spindle length is determined by centromere proteins Mis12 and Mis6 required for faithful chromosome segregation.

Authors: G Goshima; S Saitoh; M Yanagida
Journal: Genes Dev Date: 1999-07-01 Impact factor: 11.361

6. The Saccharomyces cerevisiae DNA recombination and repair functions of the RAD52 epistasis group inhibit Ty1 transposition.

Authors: A J Rattray; B K Shafer; D J Garfinkel
Journal: Genetics Date: 2000-02 Impact factor: 4.562

One-step gene disruption in yeast.

1. Stress tolerance in doughs of Saccharomyces cerevisiae trehalase mutants derived from commercial Baker's yeast.

2. The mechanism of mammalian gene replacement is consistent with the formation of long regions of heteroduplex DNA associated with two crossing-over events.

3. A fission yeast gene, him1(+)/dfp1(+), encoding a regulatory subunit for Hsk1 kinase, plays essential roles in S-phase initiation as well as in S-phase checkpoint control and recovery from DNA damage.

4. Pleiotropic alterations in lipid metabolism in yeast sac1 mutants: relationship to "bypass Sec14p" and inositol auxotrophy.

5. Proper metaphase spindle length is determined by centromere proteins Mis12 and Mis6 required for faithful chromosome segregation.

6. The Saccharomyces cerevisiae DNA recombination and repair functions of the RAD52 epistasis group inhibit Ty1 transposition.

7. Regulatory interactions between the Reg1-Glc7 protein phosphatase and the Snf1 protein kinase.

8. Inhibition of TATA-binding protein function by SAGA subunits Spt3 and Spt8 at Gcn4-activated promoters.

9. The yeast C-type cyclin Ctk2p is phosphorylated and rapidly degraded by the ubiquitin-proteasome pathway.

10. Insertional mutagenesis based on illegitimate recombination in Schizosaccharomyces pombe.