Ajay Kumar1, Vinod Kumar2, Vidya Rattan3, Vivekananda Jha4, Shalmoli Bhattacharyya5. 1. Department of Biophysics, PGIMER, Chandigarh, India; Department of Ophthalmology, University of Pittsburgh, USA. 2. Department of Nephrology, PGIMER, Chandigarh, India. 3. Unit of Oral and Maxillofacial Surgery, Oral Health Science Centre, PGIMER, Chandigarh, India. 4. Department of Nephrology, PGIMER, Chandigarh, India; The George Institute for Global Health, India. 5. Department of Biophysics, PGIMER, Chandigarh, India. Electronic address: shalmoli2007@yahoo.co.in.
Abstract
INTRODUCTION: Dental stem cells (DMSC) have been studied extensively since their early discovery. However, the data regarding osteogenic potential of DMSC with other cell types is sparse and the secretome proteins underlying these differences have not been explored. In this study, we have compared the osteogenic and adipogenic potential of DMSC with Bone Marrow Stem cells (BMSC) and reported the contribution of secretome proteins in controlling their differentiation. METHODS: Osteogenic potential of these stem cells was compared by mineralization assay, alkaline phosphatase (ALP) assay, immunofluorescence of dentine sialo phosphoprotein (DSPP) & qPCR for osteogenic genes. Adipogenic potential was compared by Oil Red O staining and qPCR for PPAR-γ, leptin & adipsin. Proteomic analysis of secretome was performed by employing WATERS nano Lc-MS/MS system. RESULTS: We observed a higher osteogenic potential in DMSC, especially dental pulp stem cells (DPSC) as compared to BMSC population but adipogenic potential was found to be better in BMSC as compared to DMSC. Deeper investigations into secretome of these cells by Lc-MS/MS revealed the presence of proteins pertaining to osteogenic and adipogenic lineage. Presence of some important proteins regulating osteogenic (DSPP, BMP7, DDR2, USP9X) and adipogenic differentiation (NCOA2, PEG10, LPA) in secretome of BMSC and DMSC reflected the role of paracrine factors during differentiation. CONCLUSION: Our study provides first evidence regarding regulation of osteogenic/adipogenic potential by secretome proteins in DMSC and BMSC. DMSC especially DPSC and its secretome show an inherent tendency for higher osteogenic differentiation and lower adipogenic differentiation, these may be potential candidates for effective future therapy in osteoporosis where disturbance of osteocyte/adipocyte homeostasis is reported.
INTRODUCTION: Dental stem cells (DMSC) have been studied extensively since their early discovery. However, the data regarding osteogenic potential of DMSC with other cell types is sparse and the secretome proteins underlying these differences have not been explored. In this study, we have compared the osteogenic and adipogenic potential of DMSC with Bone Marrow Stem cells (BMSC) and reported the contribution of secretome proteins in controlling their differentiation. METHODS: Osteogenic potential of these stem cells was compared by mineralization assay, alkaline phosphatase (ALP) assay, immunofluorescence of dentine sialo phosphoprotein (DSPP) & qPCR for osteogenic genes. Adipogenic potential was compared by Oil Red O staining and qPCR for PPAR-γ, leptin & adipsin. Proteomic analysis of secretome was performed by employing WATERS nano Lc-MS/MS system. RESULTS: We observed a higher osteogenic potential in DMSC, especially dental pulp stem cells (DPSC) as compared to BMSC population but adipogenic potential was found to be better in BMSC as compared to DMSC. Deeper investigations into secretome of these cells by Lc-MS/MS revealed the presence of proteins pertaining to osteogenic and adipogenic lineage. Presence of some important proteins regulating osteogenic (DSPP, BMP7, DDR2, USP9X) and adipogenic differentiation (NCOA2, PEG10, LPA) in secretome of BMSC and DMSC reflected the role of paracrine factors during differentiation. CONCLUSION: Our study provides first evidence regarding regulation of osteogenic/adipogenic potential by secretome proteins in DMSC and BMSC. DMSC especially DPSC and its secretome show an inherent tendency for higher osteogenic differentiation and lower adipogenic differentiation, these may be potential candidates for effective future therapy in osteoporosis where disturbance of osteocyte/adipocyte homeostasis is reported.
Authors: M B Chavez; T N Kolli; M H Tan; C Zachariadou; C Wang; M C Embree; E J Lira Dos Santos; F H Nociti; Y Wang; D N Tatakis; G Agarwal; B L Foster Journal: J Dent Res Date: 2019-10-14 Impact factor: 6.116
Authors: Yan Xu; Jing-Jing An; Dina Tabys; Yin-Dan Xie; Tian-Yu Zhao; Hao-Wei Ren; Ning Liu Journal: Int J Mol Sci Date: 2019-09-28 Impact factor: 5.923
Authors: Sara El Moshy; Israa Ahmed Radwan; Dina Rady; Marwa M S Abbass; Aiah A El-Rashidy; Khadiga M Sadek; Christof E Dörfer; Karim M Fawzy El-Sayed Journal: Stem Cells Int Date: 2020-01-31 Impact factor: 5.443