Literature DB >> 3035572

Microcell-mediated transfer of a single human chromosome complements xeroderma pigmentosum group A fibroblasts.

R A Schultz, P J Saxon, T W Glover, E C Friedberg.   

Abstract

Chromosomes from an immortalized aneuploid human fibroblast cell line were randomly tagged with the selectable marker neo by transfection with the plasmid pSV2neo. Somatic cell fusions between transfected human cells and mouse A9 cells generated pools of G418-resistant human-mouse hybrid clones containing various numbers of human chromosomes. Microcell-mediated chromosome transfer from the hybrid pools to xeroderma pigmentosum complementation group A (XP-A) cells in culture and selection for G418-resistant colonies resulted in the identification of XP cells with enhanced resistance to ultraviolet radiation. Screening of subclones from selected pools of human-mouse hybrids facilitated the identification of hybrids containing a single neo-tagged human chromosome. Transfer of this chromosome to XP-A cells (but not to XP-F or XP-C cells) results in enhanced resistance to ultraviolet light and enhanced excision repair capacity. The identification of a single human chromosome that complements the phenotype of XP-A cells in culture provides the potential for genetic mapping of the complementing gene and for its isolation by molecular cloning.

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Year:  1987        PMID： 3035572      PMCID： PMC305047          DOI： 10.1073/pnas.84.12.4176

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  11 in total

1.  Studies on gene transfer and reversion to UV resistance in xeroderma pigmentosum cells.

Authors:  R A Schultz; D P Barbis; E C Friedberg
Journal:  Somat Cell Mol Genet       Date:  1985-11

2.  Isolation and partial characterization of virus-transformed cell lines representing the A, G and variant complementation groups of xeroderma pigmentosum.

Authors:  D P Barbis; R A Schultz; E C Friedberg
Journal:  Mutat Res       Date:  1986-05       Impact factor: 2.433

3.  Selective transfer of individual human chromosomes to recipient cells.

Authors:  P J Saxon; E S Srivatsan; G V Leipzig; J H Sameshima; E J Stanbridge
Journal:  Mol Cell Biol       Date:  1985-01       Impact factor: 4.272

4.  Mechanisms of giemsa banding of chromosomes. I. Giemsa-11 banding with azure and eosin.

Authors:  H E Wyandt; D G Wysham; S K Minden; R S Anderson; F Hecht
Journal:  Exp Cell Res       Date:  1976-10-01       Impact factor: 3.905

5.  A general high-efficiency procedure for production of microcell hybrids.

Authors:  R E Fournier
Journal:  Proc Natl Acad Sci U S A       Date:  1981-10       Impact factor: 11.205

6.  DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.

Authors:  M Wigler; A Pellicer; S Silverstein; R Axel; G Urlaub; L Chasin
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

7.  Transfection of cells from a xeroderma pigmentosum patient with normal human DNA confers UV resistance.

Authors:  T Takano; M Noda; T Tamura
Journal:  Nature       Date:  1982-03-18       Impact factor: 49.962

8.  Isolation of UV-resistant revertants from a xeroderma pigmentosum complementation group A cell line.

Authors:  B Royer-Pokora; W A Haseltine
Journal:  Nature       Date:  1984 Sep 27-Oct 3       Impact factor: 49.962

9.  Integration of a dominant selectable marker into human chromosomes and transfer of marked chromosomes to mouse cells by microcell fusion.

Authors:  R S Athwal; M Smarsh; B M Searle; S S Deo
Journal:  Somat Cell Mol Genet       Date:  1985-03

10.  Microcell-mediated transfer of murine chromosomes into mouse, Chinese hamster, and human somatic cells.

Authors:  R E Fournier; F H Ruddle
Journal:  Proc Natl Acad Sci U S A       Date:  1977-01       Impact factor: 11.205
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  8 in total

1.  Elevated sister chromatid exchange phenotype of Bloom syndrome cells is complemented by human chromosome 15.

Authors:  L D McDaniel; R A Schultz
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-01       Impact factor: 11.205

2.  Functional complementation of ataxia-telangiectasia group D (AT-D) cells by microcell-mediated chromosome transfer and mapping of the AT-D locus to the region 11q22-23.

Authors:  C Lambert; R A Schultz; M Smith; C Wagner-McPherson; L D McDaniel; T Donlon; E J Stanbridge; E C Friedberg
Journal:  Proc Natl Acad Sci U S A       Date:  1991-07-01       Impact factor: 11.205

3.  Complementation of a DNA repair defect in xeroderma pigmentosum cells by transfer of human chromosome 9.

Authors:  G P Kaur; R S Athwal
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

4.  Correction of xeroderma pigmentosum complementation group D mutant cell phenotypes by chromosome and gene transfer: involvement of the human ERCC2 DNA repair gene.

Authors:  W L Flejter; L D McDaniel; D Johns; E C Friedberg; R A Schultz
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-01       Impact factor: 11.205

Review 5.  Monochromosomal Hybrids and Chromosome Transfer: A Functional Approach for Gene Identification.

Authors:  Raj P Kandpal; Arbans K Sandhu; Gurpreet Kaur; Gursurinder P Kaur; Raghbir S Athwal
Journal:  Cancer Genomics Proteomics       Date:  2017 Mar-Apr       Impact factor: 4.069

6.  Human liver mitochondrial carnitine palmitoyltransferase I: characterization of its cDNA and chromosomal localization and partial analysis of the gene.

Authors:  C H Britton; R A Schultz; B Zhang; V Esser; D W Foster; J D McGarry
Journal:  Proc Natl Acad Sci U S A       Date:  1995-03-14       Impact factor: 11.205

7.  Human chromosome 15 confers partial complementation of phenotypes to xeroderma pigmentosum group F cells.

Authors:  P J Saxon; R A Schultz; E J Stanbridge; E C Friedberg
Journal:  Am J Hum Genet       Date:  1989-04       Impact factor: 11.025

8.  Complementation of the UV-sensitive phenotype of a xeroderma pigmentosum human cell line by transfection with a cDNA clone library.

Authors:  T Teitz; T Naiman; S S Avissar; S Bar; H Okayama; D Canaani
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205
  8 in total

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