Literature DB >> 3035494

Cosmid mapping of the human chorionic gonadotropin beta subunit genes by field-inversion gel electrophoresis.

M Y Graham, T Otani, I Boime, M V Olson, G F Carle, D D Chaplin.   

Abstract

A cosmid clone containing the entire hCG beta gene cluster has been isolated. The restriction map of this clone has been determined by an indirect-end-label FIGE (field inversion gel electrophoresis) method. Analysis of this cosmid clone shows that there are 6 hCG beta genes in human genomic DNA. A previously uncloned portion of the hCG beta cluster, termed the "gap" region, has been shown not to contain any sequences homologous to the hCG beta cDNA. The restriction mapping method employed in this study takes advantage of the superior resolution of FIGE for high molecular weight DNA fragments in the size range 15-50 kb. This method is broadly applicable and permits rapid and accurate restriction mapping for extended regions of genomic DNA that have been cloned into cosmid or lambda vectors.

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Year:  1987        PMID： 3035494      PMCID： PMC340872          DOI： 10.1093/nar/15.11.4437

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  24 in total

1.  The amino acid sequence of human chorionic gonadotropin. The alpha subunit and beta subunit.

Authors:  F J Morgan; S Birken; R E Canfield
Journal:  J Biol Chem       Date:  1975-07-10       Impact factor: 5.157

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

4.  Cyclic adenosine monophosphate stimulates secretion of human chorionic gonadotropin and estrogens by human trophoblast in vitro.

Authors:  R O Hussa; M T Story; R A Pattillo
Journal:  J Clin Endocrinol Metab       Date:  1974-02       Impact factor: 5.958

5.  The primary structure of the hormone-specific, beta subunit of human pituitary luteinizing hormone (hLH).

Authors:  B Shome; A F Parlow
Journal:  J Clin Endocrinol Metab       Date:  1973-03       Impact factor: 5.958

6.  Rapid and efficient cosmid cloning.

Authors:  D Ish-Horowicz; J F Burke
Journal:  Nucleic Acids Res       Date:  1981-07-10       Impact factor: 16.971

7.  A single gonadotropin alpha-subunit gene in normal tissue and tumor-derived cell lines.

Authors:  M Boothby; R W Ruddon; C Anderson; D McWilliams; I Boime
Journal:  J Biol Chem       Date:  1981-05-25       Impact factor: 5.157

Review 8.  Glycoprotein hormones: structure and function.

Authors:  J G Pierce; T F Parsons
Journal:  Annu Rev Biochem       Date:  1981       Impact factor: 23.643

9.  The gene encoding the common alpha subunit of the four human glycoprotein hormones.

Authors:  J C Fiddes; H M Goodman
Journal:  J Mol Appl Genet       Date:  1981

10.  Recovery of DNA from gels.

Authors:  H O Smith
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600
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  10 in total

1.  Field inversion gel electrophoresis with different pulse time ramps.

Authors:  C Heller; F M Pohl
Journal:  Nucleic Acids Res       Date:  1990-11-11       Impact factor: 16.971

2.  A systematic study of field inversion gel electrophoresis.

Authors:  C Heller; F M Pohl
Journal:  Nucleic Acids Res       Date:  1989-08-11       Impact factor: 16.971

3.  Genome organization of the anaerobic pathogen Clostridium perfringens.

Authors:  B Canard; S T Cole
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

4.  The basis of high resolution separation of small DNAs by asymmetric-voltage field inversion electrophoresis and its application to DNA sequencing gels.

Authors:  B W Birren; M I Simon; E Lai
Journal:  Nucleic Acids Res       Date:  1990-03-25       Impact factor: 16.971

5.  Isolation and characterization of the human chorionic gonadotropin beta subunit (CG beta) gene cluster: regulation of transcriptionally active CG beta gene by cyclic AMP.

Authors:  J L Jameson; C M Lindell
Journal:  Mol Cell Biol       Date:  1988-12       Impact factor: 4.272

6.  The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the strain.

Authors:  E Cornillot; R V Nair; E T Papoutsakis; P Soucaille
Journal:  J Bacteriol       Date:  1997-09       Impact factor: 3.490

7.  Cloning and in vivo expression of the human GART gene using yeast artificial chromosomes.

Authors:  A Gnirke; T S Barnes; D Patterson; D Schild; T Featherstone; M V Olson
Journal:  EMBO J       Date:  1991-07       Impact factor: 11.598

8.  The danger of studying human chorionic gonadotrophin action or expression in animal models.

Authors:  R K Iles
Journal:  Br J Cancer       Date:  1991-05       Impact factor: 7.640

9.  The murine MHC class I genes, H-2Dq and H-2Lq, are strikingly homologous to each other, H-2Ld, and two genes reported to encode tumor-specific antigens.

Authors:  D R Lee; R J Rubocki; W R Lie; T H Hansen
Journal:  J Exp Med       Date:  1988-11-01       Impact factor: 14.307

10.  Molecular evidence that the H-2D and H-2L genes arose by duplication. Differences between the evolution of the class I genes in mice and humans.

Authors:  R J Rubocki; D R Lee; W R Lie; N B Myers; T H Hansen
Journal:  J Exp Med       Date:  1990-06-01       Impact factor: 14.307
  10 in total

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