Literature DB >> 3029695

Expression and identification of immunity determinants on linear DNA killer plasmids pGKL1 and pGKL2 in Kluyveromyces lactis.

M Tokunaga, N Wada, F Hishinuma.   

Abstract

The linear dsDNA plasmids, pGKL1 (8.9 kb) and pGKL2 (13.4 kb) discovered in Kluyveromyces lactis, confer killer and immunity characteristics upon various yeast strains. We have devised an immunity assay and have been able to show the expression of an immunity phenotype in the K. lactis transformants harbouring conventional circular plasmids which contain DNA fragments of pGKL1. Using this expression system, the immunity determinant on pGKL1 was identified as ORF5. In addition, the presence of pGKL2 was proved to be essential for the expression of the immunity phenotype. This is the first demonstration of this new pGKL2 function, as distinct from its known functions for the replication and maintenance of pGKL1 in yeast cells.

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Year:  1987        PMID： 3029695      PMCID： PMC340506          DOI： 10.1093/nar/15.3.1031

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  24 in total

1.  Identification of prolipoprotein signal peptidase and genomic organization of the lsp gene in Escherichia coli.

Authors:  M Tokunaga; J M Loranger; S Y Chang; M Regue; S Chang; H C Wu
Journal:  J Biol Chem       Date:  1985-05-10       Impact factor: 5.157

2.  Yeast killer toxin: site-directed mutations implicate the precursor protein as the immunity component.

Authors:  C Boone; H Bussey; D Greene; D Y Thomas; T Vernet
Journal:  Cell       Date:  1986-07-04       Impact factor: 41.582

3.  The yeast linear DNA killer plasmids, pGKL1 and pGKL2, possess terminally attached proteins.

Authors:  Y Kikuchi; K Hirai; F Hishinuma
Journal:  Nucleic Acids Res       Date:  1984-07-25       Impact factor: 16.971

Review 4.  Double-stranded ribonucleic acid killer systems in yeasts.

Authors:  D J Tipper; K A Bostian
Journal:  Microbiol Rev       Date:  1984-06

5.  Cloning and nucleotide sequences of the linear DNA killer plasmids from yeast.

Authors:  F Hishinuma; K Nakamura; K Hirai; R Nishizawa; N Gunge; T Maeda
Journal:  Nucleic Acids Res       Date:  1984-10-11       Impact factor: 16.971

6.  Transformation of Kluyveromyces lactis with the kanamycin (G418) resistance gene of Tn903.

Authors:  K Sreekrishna; T D Webster; R C Dickson
Journal:  Gene       Date:  1984-04       Impact factor: 3.688

7.  Transfer of DNA killer plasmids from Kluyveromyces lactis to Kluyveromyces fragilis and Candida pseudotropicalis.

Authors:  Y Sugisaki; N Gunge; K Sakaguchi; M Yamasaki; G Tamura
Journal:  J Bacteriol       Date:  1985-12       Impact factor: 3.490

8.  Expression of a cDNA derived from the yeast killer preprotoxin gene: implications for processing and immunity.

Authors:  S D Hanes; V E Burn; S L Sturley; D J Tipper; K A Bostian
Journal:  Proc Natl Acad Sci U S A       Date:  1986-03       Impact factor: 11.205

9.  Nucleotide sequence and transcription analysis of a linear DNA plasmid associated with the killer character of the yeast Kluyveromyces lactis.

Authors:  M J Stark; A J Mileham; M A Romanos; A Boyd
Journal:  Nucleic Acids Res       Date:  1984-08-10       Impact factor: 16.971

10.  Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors.

Authors:  B Nilsson; L Abrahmsén; M Uhlén
Journal:  EMBO J       Date:  1985-04       Impact factor: 11.598
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  24 in total

Review 1.  Yeast killer systems.

Authors:  W Magliani; S Conti; M Gerloni; D Bertolotti; L Polonelli
Journal:  Clin Microbiol Rev       Date:  1997-07       Impact factor: 26.132

2.  Genome organization of the linear plasmid, pSKL, isolated from Saccharomyces kluyveri.

Authors:  F Hishinuma; K Hirai
Journal:  Mol Gen Genet       Date:  1991-04

3.  Pichia acaciae killer system: genetic analysis of toxin immunity.

Authors:  John P Paluszynski; Roland Klassen; Friedhelm Meinhardt
Journal:  Appl Environ Microbiol       Date:  2007-05-04       Impact factor: 4.792

Review 4.  Linear plasmids among eukaryotes: fundamentals and application.

Authors:  F Meinhardt; F Kempken; J Kämper; K Esser
Journal:  Curr Genet       Date:  1990-02       Impact factor: 3.886

Review 5.  Invertrons, a class of structurally and functionally related genetic elements that includes linear DNA plasmids, transposable elements, and genomes of adeno-type viruses.

Authors:  K Sakaguchi
Journal:  Microbiol Rev       Date:  1990-03

6.  Tools and systems for evolutionary engineering of biomolecules and microorganisms.

Authors:  Sungho Jang; Minsun Kim; Jaeseong Hwang; Gyoo Yeol Jung
Journal:  J Ind Microbiol Biotechnol       Date:  2019-05-27       Impact factor: 3.346

Review 7.  Deoxyribonucleic acid plasmids in yeasts.

Authors:  F C Volkert; D W Wilson; J R Broach
Journal:  Microbiol Rev       Date:  1989-09

8.  In vivo construction of linear vectors based on killer plasmids from Kluyveromyces lactis: selection of a nuclear gene results in attachment of telomeres.

Authors:  J Kämper; F Meinhardt; N Gunge; K Esser
Journal:  Mol Cell Biol       Date:  1989-09       Impact factor: 4.272

9.  Kluyveromyces lactis killer system: ORF1 of pGKL2 has no function in immunity expression and is dispensable for killer plasmid replication and maintenance.

Authors:  R Schaffrath; M J Stark; N Gunge; F Meinhardt
Journal:  Curr Genet       Date:  1992-04       Impact factor: 3.886

10.  Osmophilic linear plasmids from the salt-tolerant yeast Debaryomyces hansenii.

Authors:  N Gunge; K Fukuda; S Morikawa; K Murakami; M Takeda; A Miwa
Journal:  Curr Genet       Date:  1993 May-Jun       Impact factor: 3.886
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