Literature DB >> 3029672

The sequence of the Saccharomyces cerevisiae gene PHO2 codes for a regulatory protein with unusual aminoacid composition.

C Sengstag, A Hinnen.   

Abstract

A new centromere vector for the construction of a Saccharomyces cerevisiae gene library, allowing direct selection for DNA insert, will be described. From that library the gene for the regulatory protein PHO2 involved in PHO5 induction has been cloned by complementation of a pho2 mutation. The complementing activity was shown to be located on a 3.6 kb HindIII fragment. This fragment was used to evict the genomic copy and with appropriate genetic crosses we proved, that the cloned gene is PHO2. The DNA sequence of PHO2 was determined. Analysis of the sequence data uncovered striking homology regions with PHO4, another protein necessary for the induction of PHO5. The relevance of the observed homology will be discussed.

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Year:  1987        PMID： 3029672      PMCID： PMC340407          DOI： 10.1093/nar/15.1.233

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  16 in total

1.  Functional selection and analysis of yeast centromeric DNA.

Authors:  P Hieter; D Pridmore; J H Hegemann; M Thomas; R W Davis; P Philippsen
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2.  Codon usage in yeast: cluster analysis clearly differentiates highly and lowly expressed genes.

Authors:  P M Sharp; T M Tuohy; K R Mosurski
Journal:  Nucleic Acids Res       Date:  1986-07-11       Impact factor: 16.971

3.  Promoter sequences of eukaryotic protein-coding genes.

Authors:  J Corden; B Wasylyk; A Buchwalder; P Sassone-Corsi; C Kedinger; P Chambon
Journal:  Science       Date:  1980-09-19       Impact factor: 47.728

4.  A position effect in the control of transcription at yeast mating type loci.

Authors:  K A Nasmyth; K Tatchell; B D Hall; C Astell; M Smith
Journal:  Nature       Date:  1981-01-22       Impact factor: 49.962

5.  Structural comparison of two nontandemly repeated yeast glyceraldehyde-3-phosphate dehydrogenase genes.

Authors:  J P Holland; M J Holland
Journal:  J Biol Chem       Date:  1980-03-25       Impact factor: 5.157

6.  Isolation, physical characterization and expression analysis of the Saccharomyces cerevisiae positive regulatory gene PHO4.

Authors:  M Legrain; M De Wilde; F Hilger
Journal:  Nucleic Acids Res       Date:  1986-04-11       Impact factor: 16.971

7.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

8.  One-step gene replacement in yeast by cotransformation.

Authors:  H Rudolph; I Koenig-Rauseo; A Hinnen
Journal:  Gene       Date:  1985       Impact factor: 3.688

9.  Specific DNA binding of GAL4, a positive regulatory protein of yeast.

Authors:  E Giniger; S M Varnum; M Ptashne
Journal:  Cell       Date:  1985-04       Impact factor: 41.582

10.  A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.

Authors:  J D Boeke; F LaCroute; G R Fink
Journal:  Mol Gen Genet       Date:  1984
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  45 in total

1.  An in vitro system recapitulates chromatin remodeling at the PHO5 promoter.

Authors:  E S Haswell; E K O'Shea
Journal:  Mol Cell Biol       Date:  1999-04       Impact factor: 4.272

2.  The Saccharomyces cerevisiae SIN3 gene, a negative regulator of HO, contains four paired amphipathic helix motifs.

Authors:  H Wang; I Clark; P R Nicholson; I Herskowitz; D J Stillman
Journal:  Mol Cell Biol       Date:  1990-11       Impact factor: 4.272

3.  Removal of N-glycosylation sites of the yeast acid phosphatase severely affects protein folding.

Authors:  M A Riederer; A Hinnen
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

4.  In vitro reconstitution of PHO5 promoter chromatin remodeling points to a role for activator-nucleosome competition in vivo.

Authors:  Franziska Ertel; A Barbara Dirac-Svejstrup; Christina Bech Hertel; Dorothea Blaschke; Jesper Q Svejstrup; Philipp Korber
Journal:  Mol Cell Biol       Date:  2010-06-21       Impact factor: 4.272

5.  Molecular analysis of a temperature sensitive allele of the PHO2 gene of Saccharomyces cerevisiae.

Authors:  B J McCarthy; C L Creasy; L W Bergman
Journal:  Nucleic Acids Res       Date:  1991-06-25       Impact factor: 16.971

6.  The acid phosphatase genes PHO10 and PHO11 in S. cerevisiae are located at the telomeres of chromosomes VIII and I.

Authors:  U Venter; W Hörz
Journal:  Nucleic Acids Res       Date:  1989-02-25       Impact factor: 16.971

7.  HOY1, a homeo gene required for hyphal formation in Yarrowia lipolytica.

Authors:  J C Torres-Guzmán; A Domínguez
Journal:  Mol Cell Biol       Date:  1997-11       Impact factor: 4.272

8.  MET4, a leucine zipper protein, and centromere-binding factor 1 are both required for transcriptional activation of sulfur metabolism in Saccharomyces cerevisiae.

Authors:  D Thomas; I Jacquemin; Y Surdin-Kerjan
Journal:  Mol Cell Biol       Date:  1992-04       Impact factor: 4.272

9.  The two positively acting regulatory proteins PHO2 and PHO4 physically interact with PHO5 upstream activation regions.

Authors:  K Vogel; W Hörz; A Hinnen
Journal:  Mol Cell Biol       Date:  1989-05       Impact factor: 4.272

10.  GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the high-osmolarity glycerol response pathway.

Authors:  J Albertyn; S Hohmann; J M Thevelein; B A Prior
Journal:  Mol Cell Biol       Date:  1994-06       Impact factor: 4.272
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