Literature DB >> 3029184

Sensitive in situ hybridisation technique using biotin-streptavidin-polyalkaline phosphatase complex.

F A Lewis, S Griffiths, R Dunnicliff, M Wells, N Dudding, C C Bird.   

Abstract

A sensitive in situ hybridisation technique, using a biotin-streptavidin-polyalkaline phosphatase complex detection system, was successfully applied to smears of fresh cultured cells, frozen sections, and formalin fixed paraffin processed tissue: the procedure was successful for DNA-DNA hybridizations using a variety of DNA probes. The detection method is rapid, reliable, and economical producing a purplish-blue precipitate at the site of hybridisation and clearly visible by low power light microscopy.

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Year:  1987        PMID： 3029184      PMCID： PMC1140860          DOI： 10.1136/jcp.40.2.163

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  10 in total

1.  Sensitive system for visualising biotinylated DNA probes hybridised in situ: rapid sex determination of intact cells.

Authors:  J Burns; V T Chan; J A Jonasson; K A Fleming; S Taylor; J O McGee
Journal:  J Clin Pathol       Date:  1985-10       Impact factor: 3.411

2.  Molecular cloning of viral DNA from human genital warts.

Authors:  E M de Villiers; L Gissmann; H zur Hausen
Journal:  J Virol       Date:  1981-12       Impact factor: 5.103

3.  Characterisation of a human Y chromosome repeated sequence and related sequences in higher primates.

Authors:  H J Cooke; J Schmidtke; J R Gosden
Journal:  Chromosoma       Date:  1982       Impact factor: 4.316

4.  Viral diagnosis by in situ hybridization. Description of a rapid simplified colorimetric method.

Authors:  E R Unger; L R Budgeon; D Myerson; D J Brigati
Journal:  Am J Surg Pathol       Date:  1986-01       Impact factor: 6.394

5.  Molecular cloning and characterization of human papilloma virus DNA derived from a laryngeal papilloma.

Authors:  L Gissmann; V Diehl; H J Schultz-Coulon; H zur Hausen
Journal:  J Virol       Date:  1982-10       Impact factor: 5.103

6.  Specific identification of human papillomavirus type in cervical smears and paraffin sections by in situ hybridization with radioactive probes: a preliminary communication.

Authors:  J Gupta; H E Gendelman; Z Naghashfar; P Gupta; N Rosenshein; E Sawada; J D Woodruff; K Shah
Journal:  Int J Gynecol Pathol       Date:  1985       Impact factor: 2.762

7.  Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes.

Authors:  D J Brigati; D Myerson; J J Leary; B Spalholz; S Z Travis; C K Fong; G D Hsiung; D C Ward
Journal:  Virology       Date:  1983-04-15       Impact factor: 3.616

8.  A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions.

Authors:  M Dürst; L Gissmann; H Ikenberg; H zur Hausen
Journal:  Proc Natl Acad Sci U S A       Date:  1983-06       Impact factor: 11.205

9.  Human V kappa immunoglobulin gene number: implications for the origin of antibody diversity.

Authors:  D L Bentley; T H Rabbitts
Journal:  Cell       Date:  1981-06       Impact factor: 41.582

10.  Immunocytological and biochemical characterization of a new neuronal cell surface component (L1 antigen) which is involved in cell adhesion.

Authors:  F G Rathjen; M Schachner
Journal:  EMBO J       Date:  1984-01       Impact factor: 11.598
  10 in total
  22 in total

Review 1.  Molecular biology made easy. The polymerase chain reaction.

Authors:  A M Clarke; N P Mapstone; P Quirke
Journal:  Histochem J       Date:  1992-12

2.  Quantitative aspects of an in situ hybridization procedure for detecting mRNAs in cells using 96-well microplates.

Authors:  H Zreiqat; R Sungaran; C R Howlett; B Markovic
Journal:  Mol Biotechnol       Date:  1998-10       Impact factor: 2.695

3.  Localization of cytomegalovirus DNA in plastic-embedded sections by in situ hybridization. A methodologic study.

Authors:  M Cao; J H Beckstead
Journal:  Am J Pathol       Date:  1989-02       Impact factor: 4.307

4.  In situ hybridization technique using an immunogold silver staining system.

Authors:  P Jackson; F A Lewis; M Wells
Journal:  Histochem J       Date:  1989-07

5.  Detection of viral DNA by in situ hybridization using bromodeoxyuridine-labeled DNA probes.

Authors:  G Niedobitek; T Finn; H Herbst; G Bornhöft; J Gerdes; H Stein
Journal:  Am J Pathol       Date:  1988-04       Impact factor: 4.307

6.  Gold induced enterocolitis complicated by cytomegalovirus infection: a previously unrecognised association.

Authors:  V Wong; J Wyatt; F Lewis; P Howdle
Journal:  Gut       Date:  1993-07       Impact factor: 23.059

7.  The importance of fixation procedures on DNA template and its suitability for solution-phase polymerase chain reaction and PCR in situ hybridization.

Authors:  J J O'Leary; G Browne; R J Landers; M Crowley; I B Healy; J T Street; A M Pollock; J Murphy; M I Johnson; F A Lewis
Journal:  Histochem J       Date:  1994-04

8.  In situ hybridisation and direct fluorescence antibodies for the detection of Chlamydia trachomatis in synovial tissue from patients with reactive arthritis.

Authors:  J Berlau; U Junker; A Groh; E Straube
Journal:  J Clin Pathol       Date:  1998-11       Impact factor: 3.411

9.  PCR in situ hybridisation detection of HPV 16 in fixed CaSki and fixed SiHa cell lines.

Authors:  J J O'Leary; G Browne; M I Johnson; R J Landers; M Crowley; I Healy; J T Street; A M Pollock; F A Lewis; A Andrew
Journal:  J Clin Pathol       Date:  1994-10       Impact factor: 3.411

10.  Epstein-Barr virus in normal, pre-malignant, and malignant lesions of the uterine cervix.

Authors:  R J Landers; J J O'Leary; M Crowley; I Healy; P Annis; L Burke; D O'Brien; J Hogan; W F Kealy; F A Lewis
Journal:  J Clin Pathol       Date:  1993-10       Impact factor: 3.411
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