Literature DB >> 2833102

Detection of viral DNA by in situ hybridization using bromodeoxyuridine-labeled DNA probes.

G Niedobitek1, T Finn, H Herbst, G Bornhöft, J Gerdes, H Stein.   

Abstract

Detection of viral DNA in infected tissues is demonstrated by in situ nucleic acid hybridization employing bromodeoxyuridine (BrdUrd)-labeled probes. BrdUrd is used alternatively to biotinylated nucleotides in the preparation of DNA probes and, after hybridization, developed with a monoclonal anti-BrdUrd antibody and the alkaline phosphatase-anti-alkaline phosphatase technique. The sensitivity using both BrdUrd- and biotin-labeled probes is similar. Furthermore, the simultaneous detection of viral coinfections will be possible by means of this technique in conjunction with biotin-labeled DNA probes.

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Year:  1988        PMID： 2833102      PMCID： PMC1880577     

Source DB:  PubMed          Journal:  Am J Pathol        ISSN： 0002-9440            Impact factor:   4.307


  18 in total

1.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

Review 2.  What's new in "in situ hybridization".

Authors:  H Höfler
Journal:  Pathol Res Pract       Date:  1987-06       Impact factor: 3.250

3.  Molecular cloning of viral DNA from human genital warts.

Authors:  E M de Villiers; L Gissmann; H zur Hausen
Journal:  J Virol       Date:  1981-12       Impact factor: 5.103

4.  Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems.

Authors:  G S Wood; R Warnke
Journal:  J Histochem Cytochem       Date:  1981-10       Impact factor: 2.479

5.  Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine.

Authors:  F Dolbeare; H Gratzner; M G Pallavicini; J W Gray
Journal:  Proc Natl Acad Sci U S A       Date:  1983-09       Impact factor: 11.205

6.  Endogenous avidin-binding activity in human lymphoid tissue.

Authors:  D Banerjee; S Pettit
Journal:  J Clin Pathol       Date:  1984-02       Impact factor: 3.411

7.  Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.

Authors:  J J Leary; D J Brigati; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

8.  Improved section adhesion for immunocytochemistry using high molecular weight polymers of L-lysine as a slide coating.

Authors:  W M Huang; S J Gibson; P Facer; J Gu; J M Polak
Journal:  Histochemistry       Date:  1983

9.  Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication.

Authors:  H G Gratzner
Journal:  Science       Date:  1982-10-29       Impact factor: 47.728

10.  Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes.

Authors:  D J Brigati; D Myerson; J J Leary; B Spalholz; S Z Travis; C K Fong; G D Hsiung; D C Ward
Journal:  Virology       Date:  1983-04-15       Impact factor: 3.616
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  8 in total

1.  In situ detection of cytomegalovirus DNA in biopsies of AIDS patients using a hybrido-immunocytochemical assay.

Authors:  M Musiani; G Gentilomi; M Zerbini; D Gibellini; G Gallinella; S Pileri; P Baglioni; M La Placa
Journal:  Histochemistry       Date:  1990

2.  In situ DNA-RNA hybridization using in vivo bromodeoxyuridine-labeled DNA probe.

Authors:  S Kitazawa; A Takenaka; N Abe; S Maeda; M Horio; T Sugiyama
Journal:  Histochemistry       Date:  1989

Review 3.  Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part III. Proliferation in normal, injured and diseased tissue, growth factors, differentiation, DNA replication sites and in situ hybridization.

Authors:  F Dolbeare
Journal:  Histochem J       Date:  1996-08

4.  Detection of viral genomes in the liver by in situ hybridization using 35S-, bromodeoxyuridine-, and biotin-labeled probes.

Authors:  G Niedobitek; T Finn; H Herbst; H Stein
Journal:  Am J Pathol       Date:  1989-03       Impact factor: 4.307

5.  Synthesis in vitro and application of biotinylated DNA probes for human papilloma virus type 16 by utilizing the polymerase chain reaction.

Authors:  P J Day; I S Bevan; S J Gurney; L S Young; M R Walker
Journal:  Biochem J       Date:  1990-04-01       Impact factor: 3.857

6.  Dot blot hybridization assay of B19 virus DNA in clinical specimens.

Authors:  J Mori; A M Field; J P Clewley; B J Cohen
Journal:  J Clin Microbiol       Date:  1989-03       Impact factor: 5.948

7.  Elucidation of Gene Expression Patterns in the Craniofacial Tissues of Mouse Embryos by Wholemount In Situ Hybridization.

Authors:  Joshua B Studdert; Heidi Bildsoe; V Pragathi Masamsetti; Patrick P L Tam
Journal:  Methods Mol Biol       Date:  2022

8.  Comparison of 35S- and digoxigenin-labeled RNA and oligonucleotide probes for in situ hybridization. Expression of mRNA of the seminal vesicle secretion protein II and androgen receptor genes in the rat prostate.

Authors:  P Komminoth; F B Merk; I Leav; H J Wolfe; J Roth
Journal:  Histochemistry       Date:  1992-11
  8 in total

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