Literature DB >> 2477351

In situ hybridization technique using an immunogold silver staining system.

P Jackson1, F A Lewis, M Wells.   

Abstract

An in situ hybridization technique using an immunogold silver staining detection system was used to detect biotinylated DNA probes in cultured cells and in formalin-fixed paraffin-embedded tissue. The detection method is rapid, reliable and economical, producing an insoluble signal at the site of hybridization which is visible at low-power light microscopy and which can be enhanced by epipolarization microscopy.

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Year:  1989        PMID： 2477351     DOI： 10.1007/bf01789741

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  11 in total

1.  Detection of low copy human papilloma virus DNA and mRNA in routine paraffin sections of cervix by non-isotopic in situ hybridisation.

Authors:  J Burns; A K Graham; C Frank; K A Fleming; M F Evans; J O McGee
Journal:  J Clin Pathol       Date:  1987-08       Impact factor: 3.411

2.  Sensitive in situ hybridisation technique using biotin-streptavidin-polyalkaline phosphatase complex.

Authors:  F A Lewis; S Griffiths; R Dunnicliff; M Wells; N Dudding; C C Bird
Journal:  J Clin Pathol       Date:  1987-02       Impact factor: 3.411

3.  Effect fixation on T and B lymphocyte surface membrane antigen demonstration in paraffin processed tissue.

Authors:  C S Holgate; P Jackson; K Pollard; D Lunny; C C Bird
Journal:  J Pathol       Date:  1986-08       Impact factor: 7.996

4.  Immunogold-silver staining: new method of immunostaining with enhanced sensitivity.

Authors:  C S Holgate; P Jackson; P N Cowen; C C Bird
Journal:  J Histochem Cytochem       Date:  1983-07       Impact factor: 2.479

5.  High-grade intensification of the end-product of the diaminobenzidine reaction for peroxidase histochemistry.

Authors:  F Gallyas; T Görcs; I Merchenthaler
Journal:  J Histochem Cytochem       Date:  1982-02       Impact factor: 2.479

6.  Actin gene expression visualized in chicken muscle tissue culture by using in situ hybridization with a biotinated nucleotide analog.

Authors:  R H Singer; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205

7.  Localization of gold in biological tissue. A photochemical method for light and electronmicroscopy.

Authors:  G Danscher
Journal:  Histochemistry       Date:  1981

8.  Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.

Authors:  P R Langer; A A Waldrop; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1981-11       Impact factor: 11.205

9.  Specific identification of human papillomavirus type in cervical smears and paraffin sections by in situ hybridization with radioactive probes: a preliminary communication.

Authors:  J Gupta; H E Gendelman; Z Naghashfar; P Gupta; N Rosenshein; E Sawada; J D Woodruff; K Shah
Journal:  Int J Gynecol Pathol       Date:  1985       Impact factor: 2.762

10.  In situ hybridization at the electron microscope level: localization of transcripts on ultrathin sections of Lowicryl K4M-embedded tissue using biotinylated probes and protein A-gold complexes.

Authors:  M Binder; S Tourmente; J Roth; M Renaud; W J Gehring
Journal:  J Cell Biol       Date:  1986-05       Impact factor: 10.539
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  2 in total

1.  Application of 1 nm gold probes on paraffin wax sections for in situ hybridisation histochemistry.

Authors:  P Jackson; D A Dockey; F A Lewis; M Wells
Journal:  J Clin Pathol       Date:  1990-10       Impact factor: 3.411

Review 2.  In situ hybridisation in perspective.

Authors:  A Warford; I Lauder
Journal:  J Clin Pathol       Date:  1991-03       Impact factor: 3.411
  2 in total

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