Mohammad Reza Azarpira1, Mohammad Mahdi Ghilian1, Mohammad Reza Sobhan2, Masoud Mehdinezhad-Yazdi2, Kazem Aghili3, Seyed Mohsen Miresmaeili4, Hossein Neamatzadeh5. 1. Bone and Joint Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 2. Department of Orthopedics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 3. Department of Radiology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 4. Department of Biology, Science and Art University, Yazd, Iran. 5. Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Abstract
OBJECTIVE: The aim of this study was to assess the association of MTHFR and TNF-α genes polymorphisms with Legg-Calvé-Perthes' disease (LCPD) risk in the Iranian children. METHODS: A total of 45 children with LCPD and 55 healthy controls were recruited to the study. Genotyping was performed via the RFLP-PCR method and genetic risk was calculated by odds ratio (OR) with its corresponding 95% confidence interval (CI). RESULTS & CONCLUSION: Our case-control study failed to determine any association of MTHFR (677C > T and 1298A > C) and TNF-α (-308G > A and -238G > A) polymorphisms with LCPD risk. More studies with larger sample size are warranted to validate our findings.
OBJECTIVE: The aim of this study was to assess the association of MTHFR and TNF-α genes polymorphisms with Legg-Calvé-Perthes' disease (LCPD) risk in the Iranian children. METHODS: A total of 45 children with LCPD and 55 healthy controls were recruited to the study. Genotyping was performed via the RFLP-PCR method and genetic risk was calculated by odds ratio (OR) with its corresponding 95% confidence interval (CI). RESULTS & CONCLUSION: Our case-control study failed to determine any association of MTHFR (677C > T and 1298A > C) and TNF-α (-308G > A and -238G > A) polymorphisms with LCPD risk. More studies with larger sample size are warranted to validate our findings.
Authors: Daniel C Perry; David M G Machin; Daniel Pope; Colin E Bruce; Peter Dangerfield; Mary Jane Platt; Andrew J Hall Journal: Am J Epidemiol Date: 2012-01-05 Impact factor: 4.897