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Literature DB >> 3016641

Isolation of a deoxycytidylate methyl transferase capable of protecting DNA uniquely against cleavage by endonuclease R.Aqu I (isoschizomer of Ava I).

C Karreman, N Tandeau de Marsac, A de Waard.

Abstract

A sequence-specific modification methylase (M.AquI) was isolated and purified from Agmenellum quadruplicatum (Synechococcus PCC 7002). This enzyme uniquely methylates the deoxycytidylate residue in the sequence *CYCGRG indicated by the asterisk. It was shown to protect DNA against cleavage by restriction endonucleases AvaI, SmaI and XhoI, which recognize the sequences CYCGRG, CCCGGG, and CTCGAG, respectively.

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Year: 1986 PMID： 3016641 PMCID： PMC311535 DOI： 10.1093/nar/14.13.5199

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

10 in total

1. A new endonuclease recognizing the deoxynucleotide sequence CCNNGG from the cyanobacterium Synechocystis 6701.

Authors: F Calléja; N Tandeau de Marsac; T Coursin; H van Ormondt; A de Waard
Journal: Nucleic Acids Res Date: 1985-09-25 Impact factor: 16.971

2. Detection of 5-methylcytosine in DNA sequences.

Authors: H Ohmori; J I Tomizawa; A M Maxam
Journal: Nucleic Acids Res Date: 1978-05 Impact factor: 16.971

3. AQUI: a more easily purified isoschizomer of AVAI.

Authors: R H Lau; W F Doolittle
Journal: FEBS Lett Date: 1980-12-01 Impact factor: 4.124

4. The effect of site specific methylation on restriction endonuclease cleavage (update).

Authors: M McClelland
Journal: Nucleic Acids Res Date: 1983-01-11 Impact factor: 16.971

5. Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors: A M Maxam; W Gilbert
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

6. MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites.

Authors: C Waalwijk; R A Flavell
Journal: Nucleic Acids Res Date: 1978-09 Impact factor: 16.971

7. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors: C Yanisch-Perron; J Vieira; J Messing
Journal: Gene Date: 1985 Impact factor: 3.688

8. DNA methylation in thermophilic bacteria: N4-methylcytosine, 5-methylcytosine, and N6-methyladenine.

Authors: M Ehrlich; M A Gama-Sosa; L H Carreira; L G Ljungdahl; K C Kuo; C W Gehrke
Journal: Nucleic Acids Res Date: 1985-02-25 Impact factor: 16.971

9. Purification and characterization of two new modification methylases: MClaI from Caryophanon latum L and MTaqI from Thermus aquaticus YTI.

Authors: M McClelland
Journal: Nucleic Acids Res Date: 1981-12-21 Impact factor: 16.971

10. Investigation of restriction-modification enzymes from M. varians RFL19 with a new type of specificity toward modification of substrate.

Authors: V Butkus; S Klimasauskas; D Kersulyte; D Vaitkevicius; A Lebionka; A Janulaitis
Journal: Nucleic Acids Res Date: 1985-08-26 Impact factor: 16.971

10 in total

13 in total

Isolation of a deoxycytidylate methyl transferase capable of protecting DNA uniquely against cleavage by endonuclease R.Aqu I (isoschizomer of Ava I).

1. A new endonuclease recognizing the deoxynucleotide sequence CCNNGG from the cyanobacterium Synechocystis 6701.

2. Detection of 5-methylcytosine in DNA sequences.

3. AQUI: a more easily purified isoschizomer of AVAI.

4. The effect of site specific methylation on restriction endonuclease cleavage (update).

5. Sequencing end-labeled DNA with base-specific chemical cleavages.

6. MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites.

7. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

8. DNA methylation in thermophilic bacteria: N4-methylcytosine, 5-methylcytosine, and N6-methyladenine.

9. Purification and characterization of two new modification methylases: MClaI from Caryophanon latum L and MTaqI from Thermus aquaticus YTI.

10. Investigation of restriction-modification enzymes from M. varians RFL19 with a new type of specificity toward modification of substrate.

1. Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

2. Agmenellum quadruplicatum M.AquI, a novel modification methylase.

3. Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.

4. Restriction enzymes and their isoschizomers.

5. Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

6. Restriction enzymes and their isoschizomers.

Review 7. Restriction enzymes and their isoschizomers.

8. Restriction enzymes and their isoschizomers.

9. Isolation and characterization of the modification methylase M . SinI.

10. Effect of site-specific methylation on restriction endonucleases and DNA modification methyltransferases.