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Literature DB >> 2836360

Isolation and characterization of the modification methylase M . SinI.

Abstract

A sequence-specific modification methylase (M . SinI) was isolated and purified from Escherichia coli harboring a derivative of recombinant plasmid pSI4 (see accompanying manuscript: C. Karreman and A. de Waard, J. Bacteriol. 170:2527-2532, 1988), which contains a Salmonella infantis DNA insert. The enzyme uniquely methylates the internal deoxycytidylate residue in the nucleotide sequence GG(A/T)MeCC, thereby protecting DNA completely against cleavage by restriction endonuclease R . SinI or R . AvaII [GG(A/T)CC], and in part against cleavage by R . Sau96I (GGNCC).

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Year: 1988 PMID： 2836360 PMCID： PMC211167 DOI： 10.1128/jb.170.6.2533-2536.1988

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

11 in total

1. Isolation and some properties of the site-specific endonuclease and methylase Bme2161 from Bacillus megaterium 216.

Authors: N I Matvienko; V M Kramarov; D M Pachkunov
Journal: Eur J Biochem Date: 1987-06-15

2. Detection of 5-methylcytosine in DNA sequences.

Authors: H Ohmori; J I Tomizawa; A M Maxam
Journal: Nucleic Acids Res Date: 1978-05 Impact factor: 16.971

3. Cloning and complete nucleotide sequences of the type II restriction-modification genes of Salmonella infantis.

Authors: C Karreman; A de Waard
Journal: J Bacteriol Date: 1988-06 Impact factor: 3.490

4. DNA methyltransferases affecting the sequence 5'CCGG.

Authors: S Jentsch; U Günthert; T A Trautner
Journal: Nucleic Acids Res Date: 1981-06-25 Impact factor: 16.971

5. A comprehensive set of sequence analysis programs for the VAX.

Authors: J Devereux; P Haeberli; O Smithies
Journal: Nucleic Acids Res Date: 1984-01-11 Impact factor: 16.971

6. Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors: A M Maxam; W Gilbert
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

7. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.

Authors: M J Casadaban; S N Cohen
Journal: J Mol Biol Date: 1980-04 Impact factor: 5.469

8. A second site-specific restriction endonuclease from Staphylococcus aureus.

Authors: J S Sussenbach; P H Steenbergh; J A Rost; W J van Leeuwen; J D van Embden
Journal: Nucleic Acids Res Date: 1978-04 Impact factor: 16.971

9. Isolation of a deoxycytidylate methyl transferase capable of protecting DNA uniquely against cleavage by endonuclease R.Aqu I (isoschizomer of Ava I).

Authors: C Karreman; N Tandeau de Marsac; A de Waard
Journal: Nucleic Acids Res Date: 1986-07-11 Impact factor: 16.971

10. Investigation of restriction-modification enzymes from M. varians RFL19 with a new type of specificity toward modification of substrate.

Authors: V Butkus; S Klimasauskas; D Kersulyte; D Vaitkevicius; A Lebionka; A Janulaitis
Journal: Nucleic Acids Res Date: 1985-08-26 Impact factor: 16.971

11 in total

1. DNA bending induced by DNA (cytosine-5) methyltransferases.

Authors: T Raskó; C Finta; A Kiss
Journal: Nucleic Acids Res Date: 2000-08-15 Impact factor: 16.971

2. Role of DNA minor groove interactions in substrate recognition by the M.SinI and M.EcoRII DNA (cytosine-5) methyltransferases.

Authors: A Kiss; G Pósfai; G Zsurka; T Raskó; P Venetianer
Journal: Nucleic Acids Res Date: 2001-08-01 Impact factor: 16.971

10. Characterization of a complex restriction-modification system detected in Staphylococcus aureus and Streptococcus agalactiae strains isolated from infections of domestic animals.

Authors: A Godány; G Bukovská; J Farkasovská; Z Brnáková; A Dmitriev; E Tkáciková; T Ayele; I Mikula
Journal: Folia Microbiol (Praha) Date: 2004 Impact factor: 2.099

Isolation and characterization of the modification methylase M . SinI.

1. Isolation and some properties of the site-specific endonuclease and methylase Bme2161 from Bacillus megaterium 216.

2. Detection of 5-methylcytosine in DNA sequences.

3. Cloning and complete nucleotide sequences of the type II restriction-modification genes of Salmonella infantis.

4. DNA methyltransferases affecting the sequence 5'CCGG.

5. A comprehensive set of sequence analysis programs for the VAX.

6. Sequencing end-labeled DNA with base-specific chemical cleavages.

7. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.

8. A second site-specific restriction endonuclease from Staphylococcus aureus.

9. Isolation of a deoxycytidylate methyl transferase capable of protecting DNA uniquely against cleavage by endonuclease R.Aqu I (isoschizomer of Ava I).

10. Investigation of restriction-modification enzymes from M. varians RFL19 with a new type of specificity toward modification of substrate.

1. DNA bending induced by DNA (cytosine-5) methyltransferases.

2. Role of DNA minor groove interactions in substrate recognition by the M.SinI and M.EcoRII DNA (cytosine-5) methyltransferases.

3. Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

4. Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.

5. In vivo DNA protection by relaxed-specificity SinI DNA methyltransferase variants.

6. Restriction enzymes and their isoschizomers.

7. Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

8. Restriction enzymes and their isoschizomers.

9. Effect of site-specific methylation on restriction endonucleases and DNA modification methyltransferases.

10. Characterization of a complex restriction-modification system detected in Staphylococcus aureus and Streptococcus agalactiae strains isolated from infections of domestic animals.