Literature DB >> 6278447

Purification and characterization of two new modification methylases: MClaI from Caryophanon latum L and MTaqI from Thermus aquaticus YTI.

M McClelland.   

Abstract

A method for detecting Type II modification methylases and determining their methylation site by assaying the ability of methylated DNA to be cleaved by heterologous restriction enzymes is described and applied to the isolation of the restriction modification methylases from Thermus thermophilus HB8, Thermus aquaticus YTI and Caryophanon latum L. M.TaqI is shown to have a methylation specificity identical to M.ThI (TCGmeA). M.ClaI methylates at adenine and protects a subset of TthI sites indicating that it methylates the sequence ATCGmeAT. Methylation by M.ThI also protects against cleavage by SalI, XhoI and at some HindII, AccI and MboI sites.

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Year:  1981        PMID： 6278447      PMCID： PMC327642          DOI： 10.1093/nar/9.24.6795

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  14 in total

1.  Occurrence of a class II restriction endonuclease in Staphylococcus aureus.

Authors:  E E Stobberingh; R Schiphof; J S Sussenbach
Journal:  J Bacteriol       Date:  1977-08       Impact factor: 3.490

2.  A thermostable sequence-specific endonuclease from Thermus aquaticus.

Authors:  S Sato; C A Hutchinson; J I Harris
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

3.  DNA methylases of Hemophilus influenzae Rd. I. Purification and properties.

Authors:  P H Roy; H O Smith
Journal:  J Mol Biol       Date:  1973-12-25       Impact factor: 5.469

4.  Physical identity of the SV40 deoxyribonucleic acid sequence recognized by the Eco RI restriction endonuclease and modification methylase.

Authors:  A Dugaiczyk; J Hedgpeth; H W Boyer; H M Goodman
Journal:  Biochemistry       Date:  1974-01-29       Impact factor: 3.162

5.  Use of restriction enzymes to study eukaryotic DNA methylation: I. The methylation pattern in ribosomal DNA from Xenopus laevis.

Authors:  A P Bird; E M Southern
Journal:  J Mol Biol       Date:  1978-01-05       Impact factor: 5.469

6.  DNA methylation in the human gamma delta beta-globin locus in erythroid and nonerythroid tissues.

Authors:  L H van der Ploeg; R A Flavell
Journal:  Cell       Date:  1980-04       Impact factor: 41.582

7.  DNA methylation at a CCGG sequence in the large intron of the rabbit beta-globin gene: tissue-specific variations.

Authors:  C Waalwijk; R A Flavell
Journal:  Nucleic Acids Res       Date:  1978-12       Impact factor: 16.971

8.  The effect of differential methylation by Escherichia coli of plasmid DNA and phage T7 and lambda DNA on the cleavage by restriction endonuclease MboI from Moraxella bovis.

Authors:  B Dreiseikelmann; R Eichenlaub; W Wackernagel
Journal:  Biochim Biophys Acta       Date:  1979-05-24

9.  An isochizomer of TaqI from Thermus thermophilus HB8.

Authors:  S Sato; T Shinomiya
Journal:  J Biochem       Date:  1978-11       Impact factor: 3.387

10.  A DNA methylase from Thermus thermophilus HB8.

Authors:  S Sato; K Nakazawa; T Shinomiya
Journal:  J Biochem       Date:  1980-09       Impact factor: 3.387
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  26 in total

1.  Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

Authors:  M McClelland; M Nelson
Journal:  Nucleic Acids Res       Date:  1992-05-11       Impact factor: 16.971

2.  Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.

Authors:  M Nelson; M McClelland
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971

3.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1990-04-25       Impact factor: 16.971

4.  Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

Authors:  M Nelson; M McClelland
Journal:  Nucleic Acids Res       Date:  1989       Impact factor: 16.971

5.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1989       Impact factor: 16.971

6.  Enzymatic cleavage of a bacterial genome at a 10-base-pair recognition site.

Authors:  M D Weil; M McClelland
Journal:  Proc Natl Acad Sci U S A       Date:  1989-01       Impact factor: 11.205

Review 7.  The effect of site-specific methylation on restriction-modification enzymes.

Authors:  M Nelson; M McClelland
Journal:  Nucleic Acids Res       Date:  1987       Impact factor: 16.971

8.  Purification of Mbo II methylase (GAAGmA) from Moraxella bovis: site specific cleavage of DNA at nine and ten base pair sequences.

Authors:  M McClelland; M Nelson; C R Cantor
Journal:  Nucleic Acids Res       Date:  1985-10-25       Impact factor: 16.971

9.  Three different macronuclear DNAs in Oxytricha fallax share a common sequence block.

Authors:  S W Cartinhour; G A Herrick
Journal:  Mol Cell Biol       Date:  1984-05       Impact factor: 4.272

10.  A second type II restriction endonuclease from Thermus aquaticus with an unusual sequence specificity.

Authors:  D Barker; M Hoff; A Oliphant; R White
Journal:  Nucleic Acids Res       Date:  1984-07-25       Impact factor: 16.971
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