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Literature DB >> 30125268

Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.

Xiao Wang^1,2,3, Jianan Li^1,2,3, Ying Wang⁴, Bei Yang⁵, Jia Wei⁴, Jing Wu¹, Ruixuan Wang¹, Xingxu Huang¹, Jia Chen¹, Li Yang⁴.

Abstract

Base editors (BEs) enable the generation of targeted single-nucleotide mutations, but currently used rat APOBEC1-based BEs are relatively inefficient in editing cytosines in highly methylated regions or in GpC contexts. By screening a variety of APOBEC and AID deaminases, we show that human APOBEC3A-conjugated BEs and versions we engineered to have narrower editing windows can mediate efficient C-to-T base editing in regions with high methylation levels and GpC dinucleotide content.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 2018 PMID： 30125268 DOI： 10.1038/nbt.4198

Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908

22 in total

1. APOBEC3G DNA deaminase acts processively 3' --> 5' on single-stranded DNA.

Authors: Linda Chelico; Phuong Pham; Peter Calabrese; Myron F Goodman
Journal: Nat Struct Mol Biol Date: 2006-04-23 Impact factor: 15.369

2. Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion.

Authors: Yuan Zong; Yanpeng Wang; Chao Li; Rui Zhang; Kunling Chen; Yidong Ran; Jin-Long Qiu; Daowen Wang; Caixia Gao
Journal: Nat Biotechnol Date: 2017-02-27 Impact factor: 54.908

3. Family-Wide Comparative Analysis of Cytidine and Methylcytidine Deamination by Eleven Human APOBEC Proteins.

Authors: Fumiaki Ito; Yang Fu; Shen-Chi A Kao; Hanjing Yang; Xiaojiang S Chen
Journal: J Mol Biol Date: 2017-05-04 Impact factor: 5.469

4. Teleseismic S wave microseisms.

Authors: Kiwamu Nishida; Ryota Takagi
Journal: Science Date: 2016-08-26 Impact factor: 47.728

5. Base editing with a Cpf1-cytidine deaminase fusion.

Authors: Xiaosa Li; Ying Wang; Yajing Liu; Bei Yang; Xiao Wang; Jia Wei; Zongyang Lu; Yuxi Zhang; Jing Wu; Xingxu Huang; Li Yang; Jia Chen
Journal: Nat Biotechnol Date: 2018-03-19 Impact factor: 54.908

6. Highly efficient RNA-guided base editing in mouse embryos.

Authors: Kyoungmi Kim; Seuk-Min Ryu; Sang-Tae Kim; Gayoung Baek; Daesik Kim; Kayeong Lim; Eugene Chung; Sunghyun Kim; Jin-Soo Kim
Journal: Nat Biotechnol Date: 2017-02-27 Impact factor: 54.908

7. Methylcytosine and normal cytosine deamination by the foreign DNA restriction enzyme APOBEC3A.

Authors: Michael A Carpenter; Ming Li; Anurag Rathore; Lela Lackey; Emily K Law; Allison M Land; Brandon Leonard; Shivender M D Shandilya; Markus-Frederik Bohn; Celia A Schiffer; William L Brown; Reuben S Harris
Journal: J Biol Chem Date: 2012-08-15 Impact factor: 5.157

8. Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor.

Authors: Lijie Wang; Wei Xue; Lei Yan; Xiaosa Li; Jia Wei; Miaomiao Chen; Jing Wu; Bei Yang; Li Yang; Jia Chen
Journal: Cell Res Date: 2017-08-29 Impact factor: 25.617

9. Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.

Authors: Johnny H Hu; Shannon M Miller; Maarten H Geurts; Weixin Tang; Liwei Chen; Ning Sun; Christina M Zeina; Xue Gao; Holly A Rees; Zhi Lin; David R Liu
Journal: Nature Date: 2018-02-28 Impact factor: 49.962

10. A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells.

Authors: Amber St Martin; Daniel Salamango; Artur Serebrenik; Nadine Shaban; William L Brown; Francesco Donati; Uday Munagala; Silvestro G Conticello; Reuben S Harris
Journal: Nucleic Acids Res Date: 2018-08-21 Impact factor: 16.971

63 in total

Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.

1. APOBEC3G DNA deaminase acts processively 3' --> 5' on single-stranded DNA.

2. Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion.

3. Family-Wide Comparative Analysis of Cytidine and Methylcytidine Deamination by Eleven Human APOBEC Proteins.

4. Teleseismic S wave microseisms.

5. Base editing with a Cpf1-cytidine deaminase fusion.

6. Highly efficient RNA-guided base editing in mouse embryos.

7. Methylcytosine and normal cytosine deamination by the foreign DNA restriction enzyme APOBEC3A.

8. Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor.

9. Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.

10. A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells.

Review 1. Single-nucleotide editing: From principle, optimization to application.

2. Genome, Epigenome, and Transcriptome Editing via Chemical Modification of Nucleobases in Living Cells.

Review 3. Genome Editing with mRNA Encoding ZFN, TALEN, and Cas9.

4. Identifying genome-wide off-target sites of CRISPR RNA-guided nucleases and deaminases with Digenome-seq.

5. Efficient base editing in G/C-rich regions to model androgen insensitivity syndrome.

6. APOBEC3A Loop 1 Is a Determinant for Single-Stranded DNA Binding and Deamination.

7. Recent advances of genome editing and related technologies in China.

Review 8. Base Editors: Modular Tools for the Introduction of Point Mutations in Living Cells.

Review 9. Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors.

10. [Development and applications of CRISPR/Cas9 library screening technology in cancer research].