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Literature DB >> 28244995

Highly efficient RNA-guided base editing in mouse embryos.

Kyoungmi Kim¹, Seuk-Min Ryu^1,2, Sang-Tae Kim¹, Gayoung Baek¹, Daesik Kim², Kayeong Lim^1,2, Eugene Chung^1,2, Sunghyun Kim^1,2, Jin-Soo Kim^1,2.

Abstract

Base editors (BEs) composed of a cytidine deaminase fused to CRISPR-Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations with an efficiency of 44-57% and allelic frequencies of up to 100%, demonstrating an efficient method to generate mice with targeted point mutations.

Entities: Chemical Species

Mesh：

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Year: 2017 PMID： 28244995 DOI： 10.1038/nbt.3816

Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908

14 in total

1. Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity.

Authors: F Ann Ran; Patrick D Hsu; Chie-Yu Lin; Jonathan S Gootenberg; Silvana Konermann; Alexandro E Trevino; David A Scott; Azusa Inoue; Shogo Matoba; Yi Zhang; Feng Zhang
Journal: Cell Date: 2013-08-29 Impact factor: 41.582

2. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells.

Authors: Daesik Kim; Sangsu Bae; Jeongbin Park; Eunji Kim; Seokjoong Kim; Hye Ryeong Yu; Jinha Hwang; Jong-Il Kim; Jin-Soo Kim
Journal: Nat Methods Date: 2015-02-09 Impact factor: 28.547

3. Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease.

Authors: Seung Woo Cho; Sojung Kim; Jong Min Kim; Jin-Soo Kim
Journal: Nat Biotechnol Date: 2013-01-29 Impact factor: 54.908

4. Targeted mutagenesis in mice by electroporation of Cpf1 ribonucleoproteins.

Authors: Junho K Hur; Kyoungmi Kim; Kyung Wook Been; Gayoung Baek; Sunghyeok Ye; Junseok W Hur; Seuk-Min Ryu; Youn Su Lee; Jin-Soo Kim
Journal: Nat Biotechnol Date: 2016-06-06 Impact factor: 54.908

5. Generation of Targeted Point Mutations in Rice by a Modified CRISPR/Cas9 System.

Authors: Jingying Li; Yongwei Sun; Jinlu Du; Yunde Zhao; Lanqin Xia
Journal: Mol Plant Date: 2016-12-08 Impact factor: 13.164

6. Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells.

Authors: Yunqing Ma; Jiayuan Zhang; Weijie Yin; Zhenchao Zhang; Yan Song; Xing Chang
Journal: Nat Methods Date: 2016-10-10 Impact factor: 28.547

Review 7. The AID/APOBEC family of nucleic acid mutators.

Authors: Silvestro G Conticello
Journal: Genome Biol Date: 2008-06-17 Impact factor: 13.583

8. CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.

Authors: Prashant Mali; John Aach; P Benjamin Stranges; Kevin M Esvelt; Mark Moosburner; Sriram Kosuri; Luhan Yang; George M Church
Journal: Nat Biotechnol Date: 2013-08-01 Impact factor: 54.908

9. Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.

Authors: Seung Woo Cho; Sojung Kim; Yongsub Kim; Jiyeon Kweon; Heon Seok Kim; Sangsu Bae; Jin-Soo Kim
Journal: Genome Res Date: 2013-11-19 Impact factor: 9.043

10. Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases.

Authors: Sangsu Bae; Jeongbin Park; Jin-Soo Kim
Journal: Bioinformatics Date: 2014-01-24 Impact factor: 6.937

139 in total

Highly efficient RNA-guided base editing in mouse embryos.

1. Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity.

2. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells.

3. Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease.

4. Targeted mutagenesis in mice by electroporation of Cpf1 ribonucleoproteins.

5. Generation of Targeted Point Mutations in Rice by a Modified CRISPR/Cas9 System.

6. Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells.

Review 7. The AID/APOBEC family of nucleic acid mutators.

8. CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.

9. Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.

10. Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases.

Review 1. Single-nucleotide editing: From principle, optimization to application.

2. Genome, Epigenome, and Transcriptome Editing via Chemical Modification of Nucleobases in Living Cells.

3. Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy.

Review 4. Cas9 Ribonucleoprotein Complex Delivery: Methods and Applications for Neuroinflammation.

Review 5. Genome Editing with mRNA Encoding ZFN, TALEN, and Cas9.

6. Genome-wide target specificities of CRISPR RNA-guided programmable deaminases.

7. Base editing on the rise.

8. Editing the human genome: where ART and science intersect.

9. Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A.

10. Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.