| Literature DB >> 28244994 |
Yuan Zong1,2, Yanpeng Wang1,2, Chao Li1,2, Rui Zhang1, Kunling Chen1, Yidong Ran3, Jin-Long Qiu4, Daowen Wang1, Caixia Gao1.
Abstract
Targeted base editing in plants without the need for a foreign DNA donor or double-stranded DNA cleavage would accelerate genome modification and breeding in a wide array of crops. We used a CRISPR-Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 within the protospacer in both protoplasts and regenerated rice, wheat and maize plants at frequencies of up to 43.48%.Entities:
Mesh：
Substances：
Year: 2017 PMID： 28244994 DOI： 10.1038/nbt.3811
Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908