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Literature DB >> 3003741

Detection of single base-pair mismatches in DNA by chemical modification followed by electrophoresis in 15% polyacrylamide gel.

D F Novack, N J Casna, S G Fischer, J P Ford.

Abstract

We have developed a method for distinguishing fragments of DNA that contain single-base mismatches from their perfectly paired homologues. Single-stranded regions within a duplex fragment are accessible to 1-cyclohexyl-3-(2-[4-(4-methyl)morpholinyl]ethyl)carbodiimide, which reacts with unpaired guanidylate and thymidylate residues in DNA. Intact linear duplex DNA molecules do not react with carbodiimide, whereas DNA molecules containing single-base mismatches react quantitatively. After carbodiimide reaction, the DNA molecules are electrophoresed in high-percentage polyacrylamide gels so that modified and unmodified fragments can be resolved. Application of this technique should make it possible to locate and purify DNA fragments that exhibit sequence differences from those that do not; these might be used to signal phenotypic variation as well as to diagnose inherited disease.

Entities: Chemical Disease

Mesh：

Substances：

Year: 1986 PMID： 3003741 PMCID： PMC322908 DOI： 10.1073/pnas.83.3.586

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

21 in total

1. Chemical modification of simian virus 40 DNA by reaction with a water-soluble carbodiimide.

Authors: J Lebowitz; C G Garon; M C Chen; N P Salzman
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2. Carbodiimide modification of superhelical PM2 DNA: considerations regarding reaction at unpaired bases and the unwinding of superhelical DNA with chemical probes.

Authors: J Lebowitz; A K Chaudhuri; A Gonenne; G Kitos
Journal: Nucleic Acids Res Date: 1977-06 Impact factor: 16.971

3. Mung bean nuclease I. Physical, chemical, and catalytic properties.

Authors: D Kowalski; W D Kroeker; M Laskowski
Journal: Biochemistry Date: 1976-10-05 Impact factor: 3.162

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Authors: D S Holmes; M Quigley
Journal: Anal Biochem Date: 1981-06 Impact factor: 3.365

5. A versatile primer for DNA sequencing in the M13mp2 cloning system.

Authors: G Heidecker; J Messing; B Gronenborn
Journal: Gene Date: 1980-06 Impact factor: 3.688

Review 6. Possible molecular detent in the DNA structure at regulatory sequences.

Authors: P Lu; S Cheung; K Arndt
Journal: J Biomol Struct Dyn Date: 1983-10

7. Chemical modification studies and the secondary structure of HeLa cell 5.8S rRNA.

Authors: J M Kelly; B E Maden
Journal: Nucleic Acids Res Date: 1980-10-10 Impact factor: 16.971

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Authors: N W Ho; P T Gilham
Journal: Biochemistry Date: 1967-12 Impact factor: 3.162

9. Oligonucleotide directed mutagenesis of the human beta-globin gene: a general method for producing specific point mutations in cloned DNA.

Authors: R B Wallace; M Schold; M J Johnson; P Dembek; K Itakura
Journal: Nucleic Acids Res Date: 1981-08-11 Impact factor: 16.971

10. DNA sequencing with chain-terminating inhibitors.

Authors: F Sanger; S Nicklen; A R Coulson
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

17 in total

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2. Direct detection and automated sequencing of individual alleles after electrophoretic strand separation: identification of a common nonsense mutation in exon 9 of the human lipoprotein lipase gene.

Authors: A Hata; M Robertson; M Emi; J M Lalouel
Journal: Nucleic Acids Res Date: 1990-09-25 Impact factor: 16.971

3. Detection of single-base mutations by reaction of DNA heteroduplexes with a water-soluble carbodiimide followed by primer extension: application to products from the polymerase chain reaction.

Authors: A Ganguly; D J Prockop
Journal: Nucleic Acids Res Date: 1990-07-11 Impact factor: 16.971

4. Mutation detection with MutH, MutL, and MutS mismatch repair proteins.

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Journal: Proc Natl Acad Sci U S A Date: 1996-04-30 Impact factor: 11.205

5. Conformation sensitive gel electrophoresis for simple and accurate detection of mutations: comparison with denaturing gradient gel electrophoresis and nucleotide sequencing.

Authors: J Körkkö; S Annunen; T Pihlajamaa; D J Prockop; L Ala-Kokko
Journal: Proc Natl Acad Sci U S A Date: 1998-02-17 Impact factor: 11.205

6. Genomic analysis II: isolation of high molecular weight heteroduplex DNA following differential methylase protection and Formamide-PERT hybridization.

Authors: N J Casna; D F Novack; M T Hsu; J P Ford
Journal: Nucleic Acids Res Date: 1986-09-25 Impact factor: 16.971

Review 7. Detection of single base changes in nucleic acids.

Authors: R G Cotton
Journal: Biochem J Date: 1989-10-01 Impact factor: 3.857

8. Mutation detection using nucleotide analogs that alter electrophoretic mobility.

Authors: J S Kornher; K J Livak
Journal: Nucleic Acids Res Date: 1989-10-11 Impact factor: 16.971

9. Reactivity of cytosine and thymine in single-base-pair mismatches with hydroxylamine and osmium tetroxide and its application to the study of mutations.

Authors: R G Cotton; N R Rodrigues; R D Campbell
Journal: Proc Natl Acad Sci U S A Date: 1988-06 Impact factor: 11.205

10. Improving CMC-derivatization of pseudouridine in RNA for mass spectrometric detection.

Authors: Anita Durairaj; Patrick A Limbach
Journal: Anal Chim Acta Date: 2008-02-17 Impact factor: 6.558