Literature DB >> 6250947

A versatile primer for DNA sequencing in the M13mp2 cloning system.

G Heidecker, J Messing, B Gronenborn.   

Abstract

A primer for DNA sequencing by the chain-termination method in the M13mp2 cloning system was constructed and amplified. The primer was isolated as an EcoRI/AluI restriction fragment. After conversion of the AluI end into an EcoRI end the fragment was cloned in pBR325 from which it can be recovered by cleavage with EcoRI. The primer hybridizes to the single-stranded DNA of the mature M13mp2 phage next to the site of insertion thereby directing DNA synthesis along the inserted DNA.

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Year:  1980        PMID： 6250947     DOI： 10.1016/0378-1119(80)90145-6

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  95 in total

1.  Nucleotide sequence of bean golden mosaic virus and a model for gene regulation in geminiviruses.

Authors:  A J Howarth; J Caton; M Bossert; R M Goodman
Journal:  Proc Natl Acad Sci U S A       Date:  1985-06       Impact factor: 11.205

2.  Cloning, expression, and characterization of the Anabaena thioredoxin gene in Escherichia coli.

Authors:  C J Lim; F K Gleason; J A Fuchs
Journal:  J Bacteriol       Date:  1986-12       Impact factor: 3.490

3.  Rift Valley fever virus structural and nonstructural proteins: recombinant protein expression and immunoreactivity against antisera from sheep.

Authors:  Bonto Faburay; William Wilson; D Scott McVey; Barbara S Drolet; Hana Weingartl; Daniel Madden; Alan Young; Wenjun Ma; Juergen A Richt
Journal:  Vector Borne Zoonotic Dis       Date:  2013-08-20       Impact factor: 2.133

Review 4.  Cloning single-stranded DNA.

Authors:  J Messing
Journal:  Mol Biotechnol       Date:  1996-02       Impact factor: 2.695

5.  Effects of DNA base analogs on transcription termination at the tryptophan operon attenuator of EScherichia coli.

Authors:  P J Farnham; T Platt
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205

6.  Construction of a cDNA to the hamster CAD gene and its application toward defining the domain for aspartate transcarbamylase.

Authors:  K Shigesada; G R Stark; J A Maley; L A Niswander; J N Davidson
Journal:  Mol Cell Biol       Date:  1985-07       Impact factor: 4.272

7.  Structure of the yeast TAP1 protein: dependence of transcription activation on the DNA context of the target gene.

Authors:  T L Aldrich; G Di Segni; B L McConaughy; N J Keen; S Whelen; B D Hall
Journal:  Mol Cell Biol       Date:  1993-06       Impact factor: 4.272

8.  Spontaneous and mutagen-induced deletions: mechanistic studies in Salmonella tester strain TA102.

Authors:  D E Levin; L J Marnett; B N Ames
Journal:  Proc Natl Acad Sci U S A       Date:  1984-07       Impact factor: 11.205

9.  Nucleotide sequence and expression of human chromosome 21-encoded superoxide dismutase mRNA.

Authors:  L Sherman; N Dafni; J Lieman-Hurwitz; Y Groner
Journal:  Proc Natl Acad Sci U S A       Date:  1983-09       Impact factor: 11.205

10.  Asymmetric transcription of mouse mammary tumor virus genes in vivo and in vitro.

Authors:  R S Prewitt; L D Washington; M R Stallcup
Journal:  J Virol       Date:  1984-04       Impact factor: 5.103
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