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Literature DB >> 3038016

An A-ELISA to detect hepatitis A virus in estuarine samples.

Abstract

An amplified enzyme-linked immunosorbent assay (A-ELISA) for detecting and quantifying hepatitis A virus in estuarine water samples is described. The test was five times more sensitive than a standard ELISA and at least two times more sensitive than radioimmunoassay. Test sensitivity was unaffected by the procedures used to concentrate the virus in estuarine samples or by the presence of humic and tannic acids in test samples. Nonspecific reactions were not encountered with a number of enteroviruses or with a rotavirus. A high sensitivity and specificity combined with speed, low cost, and freedom from radiolabels made the A-ELISA useful for detecting hepatitis A virus in environmental samples. The virus was detected in 3 of 20 estuarine water samples examined by A-ELISA.

Entities: Chemical Species

Mesh：

Year: 1987 PMID： 3038016 PMCID： PMC203834 DOI： 10.1128/aem.53.5.1192-1195.1987

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

16 in total

1. Enzyme immunoassays in diagnostic medicine. Theory and practice.

Authors: A Voller; D E Bidwell; A Bartlett
Journal: Bull World Health Organ Date: 1976 Impact factor: 9.408

2. Organic flocculation: an efficient second-step concentration method for the detection of viruses in tap water.

Authors: E Katzenelson; B Fattal; T Hostovesky
Journal: Appl Environ Microbiol Date: 1976-10 Impact factor: 4.792

3. Enzyme-linked fluorescence assay: Ultrasensitive solid-phase assay for detection of human rotavirus.

Authors: R H Yolken; P J Stopa
Journal: J Clin Microbiol Date: 1979-09 Impact factor: 5.948

4. Ultrasensitive enzymatic radioimmunoassay: application to detection of cholera toxin and rotavirus.

Authors: C C Harris; R H Yolken; H Krokan; I C Hsu
Journal: Proc Natl Acad Sci U S A Date: 1979-10 Impact factor: 11.205

5. Comparison of sensitivity of radioimmunoassay and immune electron microscopy for detecting hepatitis A antigen in fecal extracts.

Authors: W T Hall; D W Bradley; D L Madden; D H Zimmerman; D E Brandt
Journal: Proc Soc Exp Biol Med Date: 1977-06

6. Detection of hepatitis A virus in seeded estuarine samples by hybridization with cDNA probes.

Authors: X Jiang; M K Estes; T G Metcalf; J L Melnick
Journal: Appl Environ Microbiol Date: 1986-10 Impact factor: 4.792

7. Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenyl glycoluril (Iodogen).

Authors: P R Salacinski; C McLean; J E Sykes; V V Clement-Jones; P J Lowry
Journal: Anal Biochem Date: 1981-10 Impact factor: 3.365

3. Polyethylene glycol precipitation for recovery of pathogenic viruses, including hepatitis A virus and human rotavirus, from oyster, water, and sediment samples.

Authors: G D Lewis; T G Metcalf
Journal: Appl Environ Microbiol Date: 1988-08 Impact factor: 4.792

Review 4. Review on the contamination of wastewater by COVID-19 virus: Impact and treatment.

Authors: S Lahrich; F Laghrib; A Farahi; M Bakasse; S Saqrane; M A El Mhammedi
Journal: Sci Total Environ Date: 2020-09-10 Impact factor: 10.753

4 in total

An A-ELISA to detect hepatitis A virus in estuarine samples.

1. Enzyme immunoassays in diagnostic medicine. Theory and practice.

2. Organic flocculation: an efficient second-step concentration method for the detection of viruses in tap water.

3. Enzyme-linked fluorescence assay: Ultrasensitive solid-phase assay for detection of human rotavirus.

4. Ultrasensitive enzymatic radioimmunoassay: application to detection of cholera toxin and rotavirus.

5. Comparison of sensitivity of radioimmunoassay and immune electron microscopy for detecting hepatitis A antigen in fecal extracts.

6. Detection of hepatitis A virus in seeded estuarine samples by hybridization with cDNA probes.

7. Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenyl glycoluril (Iodogen).

8. The use of avidin-biotin interaction in immunoenzymatic techniques.

9. Foodborne hepatitis A at a family reunion use of IgM-specific hepatitis a serologic testing.

10. Hepatitis A: detection by immune electron microscopy of a viruslike antigen associated with acute illness.

1. Production of cytopathology in FRhK-4 cells by BS-C-1-passaged hepatitis A virus.

2. Detection of hepatitis A virus by hybridization with single-stranded RNA probes.

3. Polyethylene glycol precipitation for recovery of pathogenic viruses, including hepatitis A virus and human rotavirus, from oyster, water, and sediment samples.

Review 4. Review on the contamination of wastewater by COVID-19 virus: Impact and treatment.