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Literature DB >> 2988516

The beta-lactamase of Enterobacter cloacae P99. Chemical properties, N-terminal sequence and interaction with 6 beta-halogenopenicillanates.

B Joris, F De Meester, M Galleni, G Reckinger, J Coyette, J M Frere, J Van Beeumen.

Abstract

The beta-lactamase of Enterobacter cloacae P99 consists of one polypeptide chain of Mr 39000 devoid of disulphide bridges and free thiol groups. It contains an unusually high proportion of tyrosine and tryptophan. The N-terminal sequence exhibits overlaps with the tryptic peptide obtained after labelling the active site with 6 beta-iodopenicillanate. The active-site serine residue is at position 64. The homology with the chromosomal beta-lactamase of Escherichia coli K 12 (ampC gene) is lower within the 25 residues of the N-terminal portion than around the active-site serine residue. The P99 beta-lactamase is inactivated by 6 beta-bromo- and 6 beta-iodo-penicillanate, with a second-order rate constant of 110-140M-1 X s-1 at 30 degrees C and pH 7.0, a value that is much lower than that observed with class-A beta-lactamases.

Entities: Chemical Species

Mesh：

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Year: 1985 PMID： 2988516 PMCID： PMC1144975 DOI： 10.1042/bj2280241

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

22 in total

1. Beta-lactamase (Enterobacter species).

Authors: G W Ross
Journal: Methods Enzymol Date: 1975 Impact factor: 1.600

2. Penicillinase active sites: labelling of serine-44 in beta-lactamase I by 6beta-bromopenicillanic acid.

Authors: V Knott-Hunziker; S G Waley; B S Orlek; P G Sammes
Journal: FEBS Lett Date: 1979-03-01 Impact factor: 4.124

3. The active site of the P99 beta-lactamase from Enterobacter cloacae.

Authors: B Joris; J Dusart; J M Frere; J van Beeumen; E L Emanuel; S Petursson; J Gagnon; S G Waley
Journal: Biochem J Date: 1984-10-01 Impact factor: 3.857

4. The pH-dependence of class B and class C beta-lactamases.

Authors: R Bicknell; V Knott-Hunziker; S G Waley
Journal: Biochem J Date: 1983-07-01 Impact factor: 3.857

5. Crystallographic data for the beta-lactamase from Enterobacter cloacae P99.

Authors: P Charlier; O Dideberg; J M Frère; P C Moews; J R Knox
Journal: J Mol Biol Date: 1983-12-05 Impact factor: 5.469

6. ampC cephalosporinase of Escherichia coli K-12 has a different evolutionary origin from that of beta-lactamases of the penicillinase type.

Authors: B Jaurin; T Grundström
Journal: Proc Natl Acad Sci U S A Date: 1981-08 Impact factor: 11.205

7. The exocellular DD-carboxypeptidase-endopeptidase from Streptomyces albus G. Purification and chemical properties.

Authors: C Duez; J M Frère; F Geurts; J M Ghuysen; L Dierickx; L Delcambe
Journal: Biochem J Date: 1978-12-01 Impact factor: 3.857

8. The complete amino acid sequence of the Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase of streptomyces albus G.

Authors: B Joris; J Van Beeumen; F Casagrande; C Gerday; J M Frère; J M Ghuysen
Journal: Eur J Biochem Date: 1983-01-17

9. The acyl-enzyme mechanism of beta-lactamase action. The evidence for class C Beta-lactamases.

Authors: V Knott-Hunziker; S Petursson; S G Waley; B Jaurin; T Grundström
Journal: Biochem J Date: 1982-11-01 Impact factor: 3.857

10. Active sites of beta-lactamases from Bacillus cereus.

Authors: H A Hill; P G Sammes; S G Waley
Journal: Philos Trans R Soc Lond B Biol Sci Date: 1980-05-16 Impact factor: 6.237

25 in total

1. Identification of residues critical for catalysis in a class C beta-lactamase by combinatorial scanning mutagenesis.

Authors: Shalom D Goldberg; William Iannuccilli; Tuan Nguyen; Jingyue Ju; Virginia W Cornish
Journal: Protein Sci Date: 2003-08 Impact factor: 6.725

2. Importance of the two tryptophan residues in the Streptomyces R61 exocellular DD-peptidase.

Authors: C Bourguignon-Bellefroid; J M Wilkin; B Joris; R T Aplin; C Houssier; F G Prendergast; J Van Beeumen; J M Ghuysen; J M Frère
Journal: Biochem J Date: 1992-03-01 Impact factor: 3.857

3. Identification and characterization of beta-lactamase inhibitor protein-II (BLIP-II) interactions with beta-lactamases using phage display.

Authors: N G Brown; T Palzkill
Journal: Protein Eng Des Sel Date: 2010-03-22 Impact factor: 1.650

The beta-lactamase of Enterobacter cloacae P99. Chemical properties, N-terminal sequence and interaction with 6 beta-halogenopenicillanates.

1. Beta-lactamase (Enterobacter species).

2. Penicillinase active sites: labelling of serine-44 in beta-lactamase I by 6beta-bromopenicillanic acid.

3. The active site of the P99 beta-lactamase from Enterobacter cloacae.

4. The pH-dependence of class B and class C beta-lactamases.

5. Crystallographic data for the beta-lactamase from Enterobacter cloacae P99.

6. ampC cephalosporinase of Escherichia coli K-12 has a different evolutionary origin from that of beta-lactamases of the penicillinase type.

7. The exocellular DD-carboxypeptidase-endopeptidase from Streptomyces albus G. Purification and chemical properties.

8. The complete amino acid sequence of the Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase of streptomyces albus G.

9. The acyl-enzyme mechanism of beta-lactamase action. The evidence for class C Beta-lactamases.

10. Active sites of beta-lactamases from Bacillus cereus.

1. Identification of residues critical for catalysis in a class C beta-lactamase by combinatorial scanning mutagenesis.

2. Importance of the two tryptophan residues in the Streptomyces R61 exocellular DD-peptidase.

3. Identification and characterization of beta-lactamase inhibitor protein-II (BLIP-II) interactions with beta-lactamases using phage display.

4. Variability of chromosomally encoded beta-lactamases from Klebsiella oxytoca.

5. The active sites of the beta-lactamases of Streptomyces cacaoi and Streptomyces albus G.

6. Saturation mutagenesis of Asn152 reveals a substrate selectivity switch in P99 cephalosporinase.

7. Ragged N-termini and other variants of class A beta-lactamases analysed by chromatofocusing.

8. 6-beta-Iodopenicillanate as a probe for the classification of beta-lactamases.

9. A survey of the kinetic parameters of class C beta-lactamases. Cephalosporins and other beta-lactam compounds.

10. A survey of the kinetic parameters of class C beta-lactamases. Penicillins.