Literature DB >> 2987824

Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family.

P Fort, L Marty, M Piechaczyk, S el Sabrouty, C Dani, P Jeanteur, J M Blanchard.   

Abstract

We have isolated and sequenced a full-length cDNA clone encoding rat glyceraldehyde-3-phosphate-dehydrogenase (GAPDH, E.C.1.2.1.12). The entire mRNA is 1269 nucleotides long exclusive of poly(A) and contains respectively 71 and 196 bases of 5' and 3' non-coding regions. Primer extension as well as S1 nuclease protection experiments clearly established that a single (or at least a highly prominent) GAPDH mRNA species is expressed in all rat tissues examined. This sequence allowed the determination of the hitherto unknown primary structure of rat GAPDH which is 333 aminoacids long. Comparison between GAPDH sequences from rat, man and chicken revealed a high degree of sequence conservation at both nucleotide and protein levels.

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Year:  1985        PMID： 2987824      PMCID： PMC341087          DOI： 10.1093/nar/13.5.1431

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  22 in total

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Review 6.  Biochemical evolution.

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7.  Conservation of the primary structure at the 3' end of 18S rRNA from eucaryotic cells.

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8.  Gene dosage effect for human triosephosphate isomerase and glyceraldehyde-3-phosphate dehydrogenase in partial trisomy 12p13 and trisomy 18p.

Authors:  F Serville; C Junien; J C Kaplan; M Gachet; J Cadoux; A Broustet
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9.  The complete sequence of a full length cDNA for human liver glyceraldehyde-3-phosphate dehydrogenase: evidence for multiple mRNA species.

Authors:  P Arcari; R Martinelli; F Salvatore
Journal:  Nucleic Acids Res       Date:  1984-12-11       Impact factor: 16.971

10.  DNA sequencing with chain-terminating inhibitors.

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  440 in total

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