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Literature DB >> 29570159

Generation of human vascularized brain organoids.

Missy T Pham¹, Kari M Pollock¹, Melanie D Rose¹, Whitney A Cary¹, Heather R Stewart¹, Ping Zhou¹, Jan A Nolta¹, Ben Waldau^1,2.

Abstract

The aim of this study was to vascularize brain organoids with a patient's own endothelial cells (ECs). Induced pluripotent stem cells (iPSCs) of one UC Davis patient were grown into whole-brain organoids. Simultaneously, iPSCs from the same patient were differentiated into ECs. On day 34, the organoid was re-embedded in Matrigel with 250 000 ECs. Vascularized organoids were grown in vitro for 3-5 weeks or transplanted into immunodeficient mice on day 54, and animals were perfused on day 68. Coating of brain organoids on day 34 with ECs led to robust vascularization of the organoid after 3-5 weeks in vitro and 2 weeks in vivo. Human CD31-positive blood vessels were found inside and in-between rosettes within the center of the organoid after transplantation. Vascularization of brain organoids with a patient's own iPSC-derived ECs is technically feasible.

Entities: Chemical

Mesh：

Substances：

Year: 2018 PMID： 29570159 PMCID： PMC6476536 DOI： 10.1097/WNR.0000000000001014

Source DB: PubMed Journal: Neuroreport ISSN： 0959-4965 Impact factor: 1.837

11 in total

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Authors: J F FEENEY; R L WATTERSON
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3. Efficient Generation of Induced Pluripotent Stem and Neural Progenitor Cells From Acutely Harvested Dura Mater Obtained During Ventriculoperitoneal Shunt Surgery.

Authors: Whitney Ann Cary; Courtney Namiko Hori; Missy Trananh Pham; Catherine Ann Nacey; Jeannine Logan McGee; Mattan Hamou; Robert F Berman; Gerhard Bauer; Jan A Nolta; Ben Waldau
Journal: World Neurosurg Date: 2015-06-11 Impact factor: 2.104

4. Expression of hypoxia-inducing factor-1 alpha and endoglin in intimal hyperplasia of the middle cerebral artery of patients with Moyamoya disease.

Authors: Yasushi Takagi; Ken-Ichiro Kikuta; Kazuhiko Nozaki; Motoaki Fujimoto; Junya Hayashi; Hirotoshi Imamura; Nobuo Hashimoto
Journal: Neurosurgery Date: 2007-02 Impact factor: 4.654

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Authors: Komal Rambani; Jelena Vukasinovic; Ari Glezer; Steve M Potter
Journal: J Neurosci Methods Date: 2009-03-28 Impact factor: 2.390

Review 6. Structural and functional adaptation to hypoxia in the rat brain.

Authors: Joseph C LaManna; Juan Carlos Chavez; Paola Pichiule
Journal: J Exp Biol Date: 2004-08 Impact factor: 3.312

7. Cerebral Organoids Recapitulate Epigenomic Signatures of the Human Fetal Brain.

Authors: Chongyuan Luo; Madeline A Lancaster; Rosa Castanon; Joseph R Nery; Juergen A Knoblich; Joseph R Ecker
Journal: Cell Rep Date: 2016-12-20 Impact factor: 9.423

8. Generation of cerebral organoids from human pluripotent stem cells.

Authors: Madeline A Lancaster; Juergen A Knoblich
Journal: Nat Protoc Date: 2014-09-04 Impact factor: 13.491

9. Cerebral organoids model human brain development and microcephaly.

Authors: Madeline A Lancaster; Magdalena Renner; Carol-Anne Martin; Daniel Wenzel; Louise S Bicknell; Matthew E Hurles; Tessa Homfray; Josef M Penninger; Andrew P Jackson; Juergen A Knoblich
Journal: Nature Date: 2013-08-28 Impact factor: 49.962

10. Human cerebral organoids recapitulate gene expression programs of fetal neocortex development.

Authors: J Gray Camp; Farhath Badsha; Marta Florio; Sabina Kanton; Tobias Gerber; Michaela Wilsch-Bräuninger; Eric Lewitus; Alex Sykes; Wulf Hevers; Madeline Lancaster; Juergen A Knoblich; Robert Lachmann; Svante Pääbo; Wieland B Huttner; Barbara Treutlein
Journal: Proc Natl Acad Sci U S A Date: 2015-12-07 Impact factor: 11.205

130 in total

Generation of human vascularized brain organoids.

Review 1. Neurovascular development: The beginning of a beautiful friendship.

2. The development of the vascular pattern within the walls of the central nervous system of the chick embryo.

3. Efficient Generation of Induced Pluripotent Stem and Neural Progenitor Cells From Acutely Harvested Dura Mater Obtained During Ventriculoperitoneal Shunt Surgery.

4. Expression of hypoxia-inducing factor-1 alpha and endoglin in intimal hyperplasia of the middle cerebral artery of patients with Moyamoya disease.

5. Culturing thick brain slices: an interstitial 3D microperfusion system for enhanced viability.

Review 6. Structural and functional adaptation to hypoxia in the rat brain.

7. Cerebral Organoids Recapitulate Epigenomic Signatures of the Human Fetal Brain.

8. Generation of cerebral organoids from human pluripotent stem cells.

9. Cerebral organoids model human brain development and microcephaly.

10. Human cerebral organoids recapitulate gene expression programs of fetal neocortex development.

Review 1. Engineering Human Brain Organoids: From Basic Research to Tissue Regeneration.

2. CEREBRAL ORGANOIDS AS A MODEL FOR GLIOBLASTOMA MULTIFORME.

Review 3. Brain organoids: advances, applications and challenges.

Review 4. Applications of Human Brain Organoids to Clinical Problems.

5. In vitro Models of the Blood-Brain Barrier: Building in physiological complexity.

6. Establishment and differentiation of long-term trophoblast organoid cultures from the human placenta.

Review 7. Brain Organoids as Tools for Modeling Human Neurodevelopmental Disorders.

8. Generation and long-term culture of advanced cerebral organoids for studying later stages of neural development.

9. Vascularized human cortical organoids (vOrganoids) model cortical development in vivo.

Review 10. Human organoids to model the developing human neocortex in health and disease.