| Literature DB >> 29507408 |
Tatsuya C Murakami1, Tomoyuki Mano1,2, Shu Saikawa3, Shuhei A Horiguchi4,5, Daichi Shigeta1, Kousuke Baba6,7, Hiroshi Sekiya8, Yoshihiro Shimizu9, Kenji F Tanaka10, Hiroshi Kiyonari11, Masamitsu Iino8,12, Hideki Mochizuki6, Kazuki Tainaka1,13, Hiroki R Ueda14,15,16.
Abstract
A three-dimensional single-cell-resolution mammalian brain atlas will accelerate systems-level identification and analysis of cellular circuits underlying various brain functions. However, its construction requires efficient subcellular-resolution imaging throughout the entire brain. To address this challenge, we developed a fluorescent-protein-compatible, whole-organ clearing and homogeneous expansion protocol based on an aqueous chemical solution (CUBIC-X). The expanded, well-cleared brain enabled us to construct a point-based mouse brain atlas with single-cell annotation (CUBIC-Atlas). CUBIC-Atlas reflects inhomogeneous whole-brain development, revealing a significant decrease in the cerebral visual and somatosensory cortical areas during postnatal development. Probabilistic activity mapping of pharmacologically stimulated Arc-dVenus reporter mouse brains onto CUBIC-Atlas revealed the existence of distinct functional structures in the hippocampal dentate gyrus. CUBIC-Atlas is shareable by an open-source web-based viewer, providing a new platform for whole-brain cell profiling.Entities:
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Year: 2018 PMID： 29507408 DOI： 10.1038/s41593-018-0109-1
Source DB: PubMed Journal: Nat Neurosci ISSN： 1097-6256 Impact factor: 24.884