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Literature DB >> 2946935

Characterization of the human p53 gene.

Abstract

Cosmid and lambda clones containing the human p53 gene were isolated and characterized in detail. The gene is 20 kilobases (kb) long and has 11 exons, the first and second exons being separated by an intron of 10 kb. Restriction fragments upstream of sequences known to be within the first identified exon were tested for promoter activity by cloning them in front of the chloramphenicol acetyltransferase gene and transfecting the resulting constructs into HeLa cells. A 0.35-kb DNA fragment was identified that had promoter activity. Results of primer extension experiments indicated that the mRNA cap site falls within this fragment, as expected. Analysis of the sequence upstream of the presumptive cap site indicated that the human p53 promoter may be of an unusual type.

Entities: Gene Species

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Year: 1986 PMID： 2946935 PMCID： PMC367661 DOI： 10.1128/mcb.6.5.1379-1385.1986

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

42 in total

1. T antigen is bound to a host protein in SV40-transformed cells.

Authors: D P Lane; L V Crawford
Journal: Nature Date: 1979-03-15 Impact factor: 49.962

2. A general method for isolation of high molecular weight DNA from eukaryotes.

Authors: N Blin; D W Stafford
Journal: Nucleic Acids Res Date: 1976-09 Impact factor: 16.971

3. Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors: W D Benton; R W Davis
Journal: Science Date: 1977-04-08 Impact factor: 47.728

4. A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors: F L Graham; A J van der Eb
Journal: Virology Date: 1973-04 Impact factor: 3.616

5. HMG CoA reductase: a negatively regulated gene with unusual promoter and 5' untranslated regions.

Authors: G A Reynolds; S K Basu; T F Osborne; D J Chin; G Gil; M S Brown; J L Goldstein; K L Luskey
Journal: Cell Date: 1984-08 Impact factor: 41.582

6. The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA.

Authors: R M Lawn; E F Fritsch; R C Parker; G Blake; T Maniatis
Journal: Cell Date: 1978-12 Impact factor: 41.582

7. Six HLA-D region alpha-chain genes on human chromosome 6: polymorphisms and associations of DC alpha-related sequences with DR types.

Authors: R S Spielman; J Lee; W F Bodmer; J G Bodmer; J Trowsdale
Journal: Proc Natl Acad Sci U S A Date: 1984-06 Impact factor: 11.205

8. DNA sequencing with chain-terminating inhibitors.

Authors: F Sanger; S Nicklen; A R Coulson
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

9. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma.

Authors: U Schneider; H U Schwenk; G Bornkamm
Journal: Int J Cancer Date: 1977-05-15 Impact factor: 7.396

10. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

Authors: J M Chirgwin; A E Przybyla; R J MacDonald; W J Rutter
Journal: Biochemistry Date: 1979-11-27 Impact factor: 3.162

85 in total

1. PCR hot start using primers with the structure of molecular beacons (hairpin-like structure).

Authors: O K Kaboev; L A Luchkina; A N Tret'iakov; A R Bahrmand
Journal: Nucleic Acids Res Date: 2000-11-01 Impact factor: 16.971

2. Mutation and expression of the p51 gene in human lung cancer.

Authors: M Tani; K Shimizu; C Kawahara; T Kohno; O Ishimoto; S Ikawa; J Yokota
Journal: Neoplasia Date: 1999-04 Impact factor: 5.715

3. Partial purification and characterization of an 80-kDa transcription factor binding to bHLH motif in the rat p53 promoter.

Authors: Minhyung Lee; Yong-beom Lim; Jong-sang Park
Journal: Mol Biol Rep Date: 2002-12 Impact factor: 2.316

Characterization of the human p53 gene.

1. T antigen is bound to a host protein in SV40-transformed cells.

2. A general method for isolation of high molecular weight DNA from eukaryotes.

3. Screening lambdagt recombinant clones by hybridization to single plaques in situ.

4. A new technique for the assay of infectivity of human adenovirus 5 DNA.

5. HMG CoA reductase: a negatively regulated gene with unusual promoter and 5' untranslated regions.

6. The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA.

7. Six HLA-D region alpha-chain genes on human chromosome 6: polymorphisms and associations of DC alpha-related sequences with DR types.

8. DNA sequencing with chain-terminating inhibitors.

9. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma.

10. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

1. PCR hot start using primers with the structure of molecular beacons (hairpin-like structure).

2. Mutation and expression of the p51 gene in human lung cancer.

3. Partial purification and characterization of an 80-kDa transcription factor binding to bHLH motif in the rat p53 promoter.

4. Involvement of multiple chromosome 17p loci in medulloblastoma tumorigenesis.

5. Detection of multiallele polymorphisms within gene sequences by GC-clamped denaturing gradient gel electrophoresis.

6. The spectrum of molecular alterations in the evolution of chronic myelocytic leukemia.

Review 7. Current status of biodosimetry based on standard cytogenetic methods.

8. PCR amplification of specific DNA sequences from routinely fixed chromosomal spreads.

9. Clinicopathological features and prognosis assessment of extranodal follicular dendritic cell sarcoma.

10. p53 immunoreactivity in cervical intraepithelial neoplasia and non-neoplastic cervical squamous epithelium.