Literature DB >> 987581

A general method for isolation of high molecular weight DNA from eukaryotes.

N Blin, D W Stafford.   

Abstract

A new method for isolation of high molecular weight DNA from eukaryotes is presented. This procedure allows preparation of DNA from a variety of tissues such as calf thymus or human placenta and from cells which were more difficult to lyse until now (e.g. Crypthecodinium cuhnii, a dinoflagellate). The DNA obtained in such a way has an average molecular weight of about 200 X 10(6) d and contains very few, if any, single strand breaks.

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Year:  1976        PMID： 987581      PMCID： PMC343085          DOI： 10.1093/nar/3.9.2303

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

1.  Isolation of large molecular weight DNA from agarose gels for further digestion by restriction enzymes.

Authors:  N Blin; A von Gabain; H Bujard
Journal:  FEBS Lett       Date:  1975-04-15       Impact factor: 4.124

2.  Sedimentation rate as a measure of molecular weight of DNA.

Authors:  E BURGI; A D HERSHEY
Journal:  Biophys J       Date:  1963-07       Impact factor: 4.033

3.  SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.

Authors:  F W STUDIER
Journal:  J Mol Biol       Date:  1965-02       Impact factor: 5.469

4.  Sedimentation properties of yeast nuclear and mitochondrial DNA.

Authors:  J Blamire; D R Cryer; D B Finkelstein; J Marmur
Journal:  J Mol Biol       Date:  1972-06-14       Impact factor: 5.469

5.  Unique double-stranded fragments of bacteriophage T5 DNA resulting from preferential shear-induced breakage at nicks.

Authors:  G S Hayward
Journal:  Proc Natl Acad Sci U S A       Date:  1974-05       Impact factor: 11.205

6.  Hydrodynamic shear breakage of DNA.

Authors:  R D Bowman; N Davidson
Journal:  Biopolymers       Date:  1972       Impact factor: 2.505

7.  Isolation of high-molecular-weight DNA from mammalian cells.

Authors:  M Gross-Bellard; P Oudet; P Chambon
Journal:  Eur J Biochem       Date:  1973-07-02

8.  The nucleic acids of Drosophila melanogaster.

Authors:  J R Hastings; K S Kirby
Journal:  Biochem J       Date:  1966-08       Impact factor: 3.857
  8 in total
  822 in total

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Authors:  M Nikaido; A P Rooney; N Okada
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-31       Impact factor: 11.205

2.  Exon shuffling mimicked in cell culture.

Authors:  A A van Rijk; W W de Jong; H Bloemendal
Journal:  Proc Natl Acad Sci U S A       Date:  1999-07-06       Impact factor: 11.205

3.  Liver-specific and seasonal expression of transgenic Atlantic salmon harboring the winter flounder antifreeze protein gene.

Authors:  C Hew; R Poon; F Xiong; S Gauthier; M Shears; M King; P Davies; G Fletcher
Journal:  Transgenic Res       Date:  1999       Impact factor: 2.788

4.  Structure of a putative sodium channel from the sea anemone Aiptasia pallida.

Authors:  G B White; A Pfahnl; S Haddock; S Lamers; R M Greenberg; P A Anderson
Journal:  Invert Neurosci       Date:  1998-03

5.  Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae.

Authors:  T Kusakabe; Y Sugimoto; Y Hirota; S Toné; Y Kawaguchi; K Koga; T Ohyama
Journal:  Mol Biol Rep       Date:  2000-03       Impact factor: 2.316

6.  Purification, characterization, and genetic analysis of Cu-containing dissimilatory nitrite reductase from a denitrifying halophilic archaeon, Haloarcula marismortui.

Authors:  H Ichiki; Y Tanaka; K Mochizuki; K Yoshimatsu; T Sakurai; T Fujiwara
Journal:  J Bacteriol       Date:  2001-07       Impact factor: 3.490

7.  Antisense silencing of the creA gene in Aspergillus nidulans.

Authors:  L F Bautista; A Aleksenko; M Hentzer; A Santerre-Henriksen; J Nielsen
Journal:  Appl Environ Microbiol       Date:  2000-10       Impact factor: 4.792

8.  Cloned pairs of variable region genes for immunoglobulin heavy chains isolated from a clone library of the entire mouse genome.

Authors:  D J Kemp; S Cory; J M Adams
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

9.  Cloning of immunoglobulin kappa light chain genes from mouse liver and myeloma MOPC 173.

Authors:  M Steinmetz; H G Zachau; B Mach
Journal:  Nucleic Acids Res       Date:  1979-07-25       Impact factor: 16.971

10.  Interval mapping and congenic strains for a blood pressure QTL on rat chromosome 13.

Authors:  Q Y Zhang; H Dene; A Y Deng; M R Garrett; H J Jacob; J P Rapp
Journal:  Mamm Genome       Date:  1997-09       Impact factor: 2.957
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