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Literature DB >> 2940230

Deletion of the spf (spot 42 RNA) gene of Escherichia coli.

Abstract

To investigate the function of spot 42 RNA, a small RNA of Escherichia coli, we constructed a strain in which spf, the structural gene for this RNA, is deleted. We achieved this by using a delta att phage lambda carrying a DNA fragment spanning the spf region but with a precise deletion of spf. By integration of this phage at the spf locus and by its subsequent excision, we were able to cross the spf deletion onto the bacterial chromosome. The fact that such a deletion could be obtained indicated that spf is not an essential gene. We did not observe any major defect in delta spf cells, although in one strain background the deletion caused a slight growth impairment.

Entities: Disease Gene Species

Mesh：

Substances：
RNA, Small Nuclear

Year: 1986 PMID： 2940230 PMCID： PMC215189 DOI： 10.1128/jb.166.3.746-750.1986

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

16 in total

1. A small, unstable RNA molecule of Escherichia coli: spot 42 RNA. II. Accumulation and distribution.

Authors: B G Sahagan; J E Dahlberg
Journal: J Mol Biol Date: 1979-07-05 Impact factor: 5.469

2. Prophage lambda at unusual chromosomal locations. II. Mutations induced by bacteriophage lambda in Escherichia coli K12.

Authors: K Shimada; R A Weisberg; M E Gottesman
Journal: J Mol Biol Date: 1973-10-25 Impact factor: 5.469

3. A small, unstable RNA molecule of Escherichia coli: spot 42 RNA. I. Nucleotide sequence analysis.

Authors: B G Sahagan; J E Dahlberg
Journal: J Mol Biol Date: 1979-07-05 Impact factor: 5.469

4. In vitro construction of bacteriophage lambda carrying segments of the Escherichia coli chromosome: selection of hybrids containing the gene for DNA ligase.

Authors: J R Cameron; S M Panasenko; I R Lehman; R W Davis
Journal: Proc Natl Acad Sci U S A Date: 1975-09 Impact factor: 11.205

5. Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I.

Authors: C M Joyce; W S Kelley; N D Grindley
Journal: J Biol Chem Date: 1982-02-25 Impact factor: 5.157

6. Interaction of cAMP receptor protein with the ompA gene, a gene for a major outer membrane protein of Escherichia coli.

Authors: R N Movva; P Green; K Nakamura; M Inouye
Journal: FEBS Lett Date: 1981-06-15 Impact factor: 4.124

7. Regulation of synthesis of a major outer membrane protein: cyclic AMP represses Escherichia coli protein III synthesis.

Authors: U Mallick; P Herrlich
Journal: Proc Natl Acad Sci U S A Date: 1979-11 Impact factor: 11.205

8. Identification of two genes immediately downstream from the polA gene of Escherichia coli.

Authors: C M Joyce; N D Grindley
Journal: J Bacteriol Date: 1982-12 Impact factor: 3.490

9. A gene between polA and glnA retards growth of Escherichia coli when present in multiple copies: physiological effects of the gene for spot 42 RNA.

Authors: P W Rice; J E Dahlberg
Journal: J Bacteriol Date: 1982-12 Impact factor: 3.490

7. Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida.

Authors: Geir A Hansen; Rafi Ahmad; Erik Hjerde; Christopher G Fenton; Nils-Peder Willassen; Peik Haugen
Journal: BMC Genomics Date: 2012-01-24 Impact factor: 3.969

7 in total

Deletion of the spf (spot 42 RNA) gene of Escherichia coli.

1. A small, unstable RNA molecule of Escherichia coli: spot 42 RNA. II. Accumulation and distribution.

2. Prophage lambda at unusual chromosomal locations. II. Mutations induced by bacteriophage lambda in Escherichia coli K12.

3. A small, unstable RNA molecule of Escherichia coli: spot 42 RNA. I. Nucleotide sequence analysis.

4. In vitro construction of bacteriophage lambda carrying segments of the Escherichia coli chromosome: selection of hybrids containing the gene for DNA ligase.

5. Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I.

6. Interaction of cAMP receptor protein with the ompA gene, a gene for a major outer membrane protein of Escherichia coli.

7. Regulation of synthesis of a major outer membrane protein: cyclic AMP represses Escherichia coli protein III synthesis.

8. Identification of two genes immediately downstream from the polA gene of Escherichia coli.

9. A gene between polA and glnA retards growth of Escherichia coli when present in multiple copies: physiological effects of the gene for spot 42 RNA.

10. Inhibitory effect of adenosine 3',5'-phosphate on cell division of Escherichia coli K-12 mutant derivatives.

1. Discriminating tastes: physiological contributions of the Hfq-binding small RNA Spot 42 to catabolite repression.

2. DNA polymerase I activity in Escherichia coli is influenced by spot 42 RNA.

3. Spot 42 RNA of Escherichia coli is not an mRNA.

Review 4. Molecular call and response: the physiology of bacterial small RNAs.

5. Spot 42 RNA mediates discoordinate expression of the E. coli galactose operon.

Review 6. The Spot 42 RNA: A regulatory small RNA with roles in the central metabolism.

7. Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida.