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Literature DB >> 29358758

Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex.

Marcello Clerici¹, Marco Faini², Lena M Muckenfuss¹, Ruedi Aebersold^2,3, Martin Jinek⁴.

Abstract

Mammalian mRNA biogenesis requires specific recognition of a hexanucleotide AAUAAA motif in the polyadenylation signals (PAS) of precursor mRNA (pre-mRNA) transcripts by the cleavage and polyadenylation specificity factor (CPSF) complex. Here we present a 3.1-Å-resolution cryo-EM structure of a core CPSF module bound to the PAS hexamer motif. The structure reveals the molecular interactions responsible for base-specific recognition, providing a rationale for mechanistic differences between mammalian and yeast 3' polyadenylation.

Entities: CellLine Chemical Disease Gene Species

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Year: 2018 PMID： 29358758 PMCID： PMC6900284 DOI： 10.1038/s41594-017-0020-6

Source DB: PubMed Journal: Nat Struct Mol Biol ISSN： 1545-9985 Impact factor: 15.369

27 in total

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3. Molecular architecture of the human pre-mRNA 3' processing complex.

Authors: Yongsheng Shi; Dafne Campigli Di Giammartino; Derek Taylor; Ali Sarkeshik; William J Rice; John R Yates; Joachim Frank; James L Manley
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4. Focus: The interface between data collection and data processing in cryo-EM.

Authors: Nikhil Biyani; Ricardo D Righetto; Robert McLeod; Daniel Caujolle-Bert; Daniel Castano-Diez; Kenneth N Goldie; Henning Stahlberg
Journal: J Struct Biol Date: 2017-03-23 Impact factor: 2.867

5. MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes.

Authors: Scott D Gradia; Justin P Ishida; Miaw-Sheue Tsai; Chris Jeans; John A Tainer; Jill O Fuss
Journal: Methods Enzymol Date: 2017-05-15 Impact factor: 1.600

6. A quantitative atlas of polyadenylation in five mammals.

Authors: Adnan Derti; Philip Garrett-Engele; Kenzie D Macisaac; Richard C Stevens; Shreedharan Sriram; Ronghua Chen; Carol A Rohl; Jason M Johnson; Tomas Babak
Journal: Genome Res Date: 2012-03-27 Impact factor: 9.043

Review 7. The end of the message: multiple protein-RNA interactions define the mRNA polyadenylation site.

Authors: Yongsheng Shi; James L Manley
Journal: Genes Dev Date: 2015-05-01 Impact factor: 11.361

8. Cleavage and polyadenylation factor CPF specifically interacts with the pre-mRNA 3' processing signal AAUAAA.

Authors: W Keller; S Bienroth; K M Lang; G Christofori
Journal: EMBO J Date: 1991-12 Impact factor: 11.598

9. CPSF30 and Wdr33 directly bind to AAUAAA in mammalian mRNA 3' processing.

Authors: Serena L Chan; Ina Huppertz; Chengguo Yao; Lingjie Weng; James J Moresco; John R Yates; Jernej Ule; James L Manley; Yongsheng Shi
Journal: Genes Dev Date: 2014-10-09 Impact factor: 11.361

10. Architecture of eukaryotic mRNA 3'-end processing machinery.

Authors: Ana Casañal; Ananthanarayanan Kumar; Chris H Hill; Ashley D Easter; Paul Emsley; Gianluca Degliesposti; Yuliya Gordiyenko; Balaji Santhanam; Jana Wolf; Katrin Wiederhold; Gillian L Dornan; Mark Skehel; Carol V Robinson; Lori A Passmore
Journal: Science Date: 2017-10-26 Impact factor: 47.728

41 in total

Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex.

1. UCSF Chimera--a visualization system for exploratory research and analysis.

2. Coot: model-building tools for molecular graphics.

3. Molecular architecture of the human pre-mRNA 3' processing complex.

4. Focus: The interface between data collection and data processing in cryo-EM.

5. MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes.

6. A quantitative atlas of polyadenylation in five mammals.

Review 7. The end of the message: multiple protein-RNA interactions define the mRNA polyadenylation site.

8. Cleavage and polyadenylation factor CPF specifically interacts with the pre-mRNA 3' processing signal AAUAAA.

9. CPSF30 and Wdr33 directly bind to AAUAAA in mammalian mRNA 3' processing.

10. Architecture of eukaryotic mRNA 3'-end processing machinery.

1. Recent molecular insights into canonical pre-mRNA 3'-end processing.

2. Transcriptome Analyses of FY Mutants Reveal Its Role in mRNA Alternative Polyadenylation.

3. Suboptimal RNA-RNA interaction limits U1 snRNP inhibition of canonical mRNA 3' processing.

Review 4. Cryo-EM in drug discovery: achievements, limitations and prospects.

Review 5. mRNA Editing, Processing and Quality Control in Caenorhabditis elegans.

6. Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m⁶A modification.

7. Structural Insights into the Human Pre-mRNA 3'-End Processing Machinery.

8. Casein Kinase 1δ Stabilizes Mature Axons by Inhibiting Transcription Termination of Ankyrin.

Review 9. Targeting mRNA processing as an anticancer strategy.

Review 10. RNA motifs and combinatorial prediction of interactions, stability and localization of noncoding RNAs.