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Literature DB >> 29296501

Observation of live chromatin dynamics in cells via 3D localization microscopy using Tetrapod point spread functions.

Yoav Shechtman^1,2,3, Anna-Karin Gustavsson^1,4, Petar N Petrov¹, Elisa Dultz⁵, Maurice Y Lee^1,6, Karsten Weis⁵, W E Moerner^1,7.

Abstract

We report the observation of chromatin dynamics in living budding yeast (Saccharomyces cerevisiae) cells, in three-dimensions (3D). Using dual color localization microscopy and employing a Tetrapod point spread function, we analyze the spatio-temporal dynamics of two fluorescently labeled DNA loci surrounding the GAL locus. From the measured trajectories, we obtain different dynamical characteristics in terms of inter-loci distance and temporal variance; when the GAL locus is activated, the 3D inter-loci distance and temporal variance increase compared to the inactive state. These changes are visible in spite of the large thermally- and biologically-driven heterogeneity in the relative motion of the two loci. Our observations are consistent with current euchromatin vs. heterochromatin models.

Entities: Species

Keywords: (070.0070) Fourier optics and signal processing; (170.1530) Cell analysis; (170.2520) Fluorescence microscopy; (170.6900) Three-dimensional microscopy

Year: 2017 PMID： 29296501 PMCID： PMC5745116 DOI： 10.1364/BOE.8.005735

Source DB: PubMed Journal: Biomed Opt Express ISSN： 2156-7085 Impact factor: 3.732

29 in total

1. Three-dimensional tracking of single mRNA particles in Saccharomyces cerevisiae using a double-helix point spread function.

Authors: Michael A Thompson; Jason M Casolari; Majid Badieirostami; Patrick O Brown; W E Moerner
Journal: Proc Natl Acad Sci U S A Date: 2010-10-04 Impact factor: 11.205

2. Optical sectioning deep inside live embryos by selective plane illumination microscopy.

Authors: Jan Huisken; Jim Swoger; Filippo Del Bene; Joachim Wittbrodt; Ernst H K Stelzer
Journal: Science Date: 2004-08-13 Impact factor: 47.728

3. Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions.

Authors: Prashant Prabhat; Sripad Ram; E Sally Ward; Raimund J Ober
Journal: IEEE Trans Nanobioscience Date: 2004-12 Impact factor: 2.935

Review 4. Genome architecture: domain organization of interphase chromosomes.

Authors: Wendy A Bickmore; Bas van Steensel
Journal: Cell Date: 2013-03-14 Impact factor: 41.582

5. Quantitative multicolor subdiffraction imaging of bacterial protein ultrastructures in three dimensions.

Authors: Andreas Gahlmann; Jerod L Ptacin; Ginni Grover; Sean Quirin; Lexy von Diezmann; Marissa K Lee; Mikael P Backlund; Lucy Shapiro; Rafael Piestun; W E Moerner
Journal: Nano Lett Date: 2013-02-25 Impact factor: 11.189

Review 6. Yeast chromatin structure and regulation of GAL gene expression.

Authors: R Bash; D Lohr
Journal: Prog Nucleic Acid Res Mol Biol Date: 2001

7. Optimal point spread function design for 3D imaging.

Authors: Yoav Shechtman; Steffen J Sahl; Adam S Backer; W E Moerner
Journal: Phys Rev Lett Date: 2014-09-26 Impact factor: 9.161

8. Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution.

Authors: Bi-Chang Chen; Wesley R Legant; Kai Wang; Lin Shao; Daniel E Milkie; Michael W Davidson; Chris Janetopoulos; Xufeng S Wu; John A Hammer; Zhe Liu; Brian P English; Yuko Mimori-Kiyosue; Daniel P Romero; Alex T Ritter; Jennifer Lippincott-Schwartz; Lillian Fritz-Laylin; R Dyche Mullins; Diana M Mitchell; Joshua N Bembenek; Anne-Cecile Reymann; Ralph Böhme; Stephan W Grill; Jennifer T Wang; Geraldine Seydoux; U Serdar Tulu; Daniel P Kiehart; Eric Betzig
Journal: Science Date: 2014-10-23 Impact factor: 47.728

9. Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions.

Authors: Yoav Shechtman; Lucien E Weiss; Adam S Backer; Steffen J Sahl; W E Moerner
Journal: Nano Lett Date: 2015-05-18 Impact factor: 11.189

10. Small-molecule labeling of live cell surfaces for three-dimensional super-resolution microscopy.

Authors: Marissa K Lee; Prabin Rai; Jarrod Williams; Robert J Twieg; W E Moerner
Journal: J Am Chem Soc Date: 2014-09-24 Impact factor: 15.419

11 in total

Review 1. Light sheet approaches for improved precision in 3D localization-based super-resolution imaging in mammalian cells [Invited].

Authors: Anna-Karin Gustavsson; Petar N Petrov; W E Moerner
Journal: Opt Express Date: 2018-05-14 Impact factor: 3.894

2. Tilted Light Sheet Microscopy with 3D Point Spread Functions for Single-Molecule Super-Resolution Imaging in Mammalian Cells.

Authors: Anna-Karin Gustavsson; Petar N Petrov; Maurice Y Lee; Yoav Shechtman; W E Moerner
Journal: Proc SPIE Int Soc Opt Eng Date: 2018-02

3. Quantifying cell-cycle-dependent chromatin dynamics during interphase by live 3D tracking.

Authors: Tal Naor; Yevgeni Nogin; Elias Nehme; Boris Ferdman; Lucien E Weiss; Onit Alalouf; Yoav Shechtman
Journal: iScience Date: 2022-04-04

4. Multi-center evaluation of artificial intelligent imaging and clinical models for predicting neoadjuvant chemotherapy response in breast cancer.

Authors: Tan Hong Qi; Ong Hiok Hian; Arjunan Muthu Kumaran; Tira J Tan; Tan Ryan Ying Cong; Ghislaine Lee Su-Xin; Elaine Hsuen Lim; Raymond Ng; Ming Chert Richard Yeo; Faye Lynette Lim Wei Tching; Zhang Zewen; Christina Yang Shi Hui; Wong Ru Xin; Su Kai Gideon Ooi; Lester Chee Hao Leong; Su Ming Tan; Madhukumar Preetha; Yirong Sim; Veronique Kiak Mien Tan; Joe Yeong; Wong Fuh Yong; Yiyu Cai; Wen Long Nei
Journal: Breast Cancer Res Treat Date: 2022-03-09 Impact factor: 4.872