Literature DB >> 29295908

Gateway Recombinational Cloning.

John S Reece-Hoyes, Albertha J M Walhout.   

Abstract

The Gateway recombinatorial cloning system was developed for cloning multiple DNA fragments in parallel (e.g., in 96-well formats) in a standardized manner using the same enzymes. Gateway cloning is based on the highly specific integration and excision reactions of bacteriophage λ into and out of the Escherichia coli genome. Because the sites of recombination ("att" sites) are much longer (25-242 bp) than restriction sites, they are extremely unlikely to occur by chance in DNA fragments. Therefore, the same recombination enzyme can be used to robustly clone many different fragments of variable size in parallel reactions.
© 2018 Cold Spring Harbor Laboratory Press.

Entities:  

Mesh：

Year:  2018        PMID： 29295908      PMCID： PMC5935001          DOI： 10.1101/pdb.top094912

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN： 1559-6095


  22 in total

1.  GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes.

Authors:  A J Walhout; G F Temple; M A Brasch; J L Hartley; M A Lorson; S van den Heuvel; M Vidal
Journal:  Methods Enzymol       Date:  2000       Impact factor: 1.600

2.  A gene-centered C. elegans protein-DNA interaction network.

Authors:  Bart Deplancke; Arnab Mukhopadhyay; Wanyuan Ao; Ahmed M Elewa; Christian A Grove; Natalia J Martinez; Reynaldo Sequerra; Lynn Doucette-Stamm; John S Reece-Hoyes; Ian A Hope; Heidi A Tissenbaum; Susan E Mango; Albertha J M Walhout
Journal:  Cell       Date:  2006-06-16       Impact factor: 41.582

3.  The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes.

Authors:  Q Liu; M Z Li; D Leibham; D Cortez; S J Elledge
Journal:  Curr Biol       Date:  1998-12-03       Impact factor: 10.834

4.  C. elegans ORFeome version 1.1: experimental verification of the genome annotation and resource for proteome-scale protein expression.

Authors:  Jérôme Reboul; Philippe Vaglio; Jean-François Rual; Philippe Lamesch; Monica Martinez; Christopher M Armstrong; Siming Li; Laurent Jacotot; Nicolas Bertin; Rekin's Janky; Troy Moore; James R Hudson; James L Hartley; Michael A Brasch; Jean Vandenhaute; Simon Boulton; Gregory A Endress; Sarah Jenna; Eric Chevet; Vasilis Papasotiropoulos; Peter P Tolias; Jason Ptacek; Mike Snyder; Raymond Huang; Mark R Chance; Hongmei Lee; Lynn Doucette-Stamm; David E Hill; Marc Vidal
Journal:  Nat Genet       Date:  2003-05       Impact factor: 38.330

5.  A first version of the Caenorhabditis elegans Promoterome.

Authors:  Denis Dupuy; Qian-Ru Li; Bart Deplancke; Mike Boxem; Tong Hao; Philippe Lamesch; Reynaldo Sequerra; Stephanie Bosak; Lynn Doucette-Stamm; Ian A Hope; David E Hill; Albertha J M Walhout; Marc Vidal
Journal:  Genome Res       Date:  2004-10       Impact factor: 9.043

6.  Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library.

Authors:  Jean-François Rual; Julian Ceron; John Koreth; Tong Hao; Anne-Sophie Nicot; Tomoko Hirozane-Kishikawa; Jean Vandenhaute; Stuart H Orkin; David E Hill; Sander van den Heuvel; Marc Vidal
Journal:  Genome Res       Date:  2004-10       Impact factor: 9.043

7.  A multiparameter network reveals extensive divergence between C. elegans bHLH transcription factors.

Authors:  Christian A Grove; Federico De Masi; M Inmaculada Barrasa; Daniel E Newburger; Mark J Alkema; Martha L Bulyk; Albertha J M Walhout
Journal:  Cell       Date:  2009-07-23       Impact factor: 41.582

8.  A map of the interactome network of the metazoan C. elegans.

Authors:  Siming Li; Christopher M Armstrong; Nicolas Bertin; Hui Ge; Stuart Milstein; Mike Boxem; Pierre-Olivier Vidalain; Jing-Dong J Han; Alban Chesneau; Tong Hao; Debra S Goldberg; Ning Li; Monica Martinez; Jean-François Rual; Philippe Lamesch; Lai Xu; Muneesh Tewari; Sharyl L Wong; Lan V Zhang; Gabriel F Berriz; Laurent Jacotot; Philippe Vaglio; Jérôme Reboul; Tomoko Hirozane-Kishikawa; Qianru Li; Harrison W Gabel; Ahmed Elewa; Bridget Baumgartner; Debra J Rose; Haiyuan Yu; Stephanie Bosak; Reynaldo Sequerra; Andrew Fraser; Susan E Mango; William M Saxton; Susan Strome; Sander Van Den Heuvel; Fabio Piano; Jean Vandenhaute; Claude Sardet; Mark Gerstein; Lynn Doucette-Stamm; Kristin C Gunsalus; J Wade Harper; Michael E Cusick; Frederick P Roth; David E Hill; Marc Vidal
Journal:  Science       Date:  2004-01-02       Impact factor: 47.728

9.  Bacteriophage P1 site-specific recombination. Purification and properties of the Cre recombinase protein.

Authors:  K Abremski; R Hoess
Journal:  J Biol Chem       Date:  1984-02-10       Impact factor: 5.157

10.  A versatile ligation-independent cloning method suitable for high-throughput expression screening applications.

Authors:  Nick S Berrow; David Alderton; Sarah Sainsbury; Joanne Nettleship; Rene Assenberg; Nahid Rahman; David I Stuart; Raymond J Owens
Journal:  Nucleic Acids Res       Date:  2007-02-22       Impact factor: 16.971
View more
  15 in total

1.  Using Multisite LR Cloning to Generate a Destination Clone.

Authors:  John S Reece-Hoyes; Albertha J M Walhout
Journal:  Cold Spring Harb Protoc       Date:  2018-01-02

2.  pGP-B2E, a Recombinant Compatible TA/TB-Ligation Vector for Rapid and Inexpensive Gene Cloning.

Authors:  Dongyue Li; Chao Zheng; Jie Zhou; Bin Chen; Rumeng Xu; Wenxia Yuan; Ersong Zheng; Weifang Liang; Yong Yang; Lijuan He; Jianghua Shi; Chengqi Yan; Xuming Wang; Jianping Chen
Journal:  Mol Biotechnol       Date:  2020-01       Impact factor: 2.695

3.  A strategy for in-house production of a positive selection cloning vector from the commercial pJET1.2/blunt cloning vector at minimal cost.

Authors:  Omar Nawawi; Mohd Puad Abdullah; Chong Yu Lok Yusuf
Journal:  3 Biotech       Date:  2022-08-09       Impact factor: 2.893

Review 4.  Reconstruction of human genome evolution in yeast: an educational primer for use with "systematic humanization of the yeast cytoskeleton discerns functionally replaceable from divergent human genes".

Authors:  Zuzana Brzáčová; Mária Peťková; Katarína Veljačiková; Terézia Zajičková; Ľubomír Tomáška
Journal:  Genetics       Date:  2021-10-02       Impact factor: 4.402

5.  TrichoGate: An Improved Vector System for a Large Scale of Functional Analysis of Trichoderma Genes.

Authors:  Guillermo Nogueira-López; Fabiola Padilla-Arizmendi; Sarah Inwood; Sarah Lyne; Johanna M Steyaert; Maria Fernanda Nieto-Jacobo; Alison Stewart; Artemio Mendoza-Mendoza
Journal:  Front Microbiol       Date:  2019-12-10       Impact factor: 5.640

6.  Construction of a Full-Length cDNA Over-Expressing Library to Identify Valuable Genes from Populus tomentosa.

Authors:  Lingfei Kong; Zeyu Li; Qin Song; Xiaohong Li; Keming Luo
Journal:  Int J Mol Sci       Date:  2021-03-26       Impact factor: 5.923

7.  Gene Expression Profiling of Different Huh7 Variants Reveals Novel Hepatitis C Virus Host Factors.

Authors:  Christopher Dächert; Evgeny Gladilin; Marco Binder
Journal:  Viruses       Date:  2019-12-28       Impact factor: 5.048

8.  Chitosan primes plant defence mechanisms against Botrytis cinerea, including expression of Avr9/Cf-9 rapidly elicited genes.

Authors:  Daniel De Vega; Nicola Holden; Pete E Hedley; Jenny Morris; Estrella Luna; Adrian Newton
Journal:  Plant Cell Environ       Date:  2020-11-04       Impact factor: 7.228

Review 9.  Principles and Practical Considerations for the Analysis of Disease-Associated Alternative Splicing Events Using the Gateway Cloning-Based Minigene Vectors pDESTsplice and pSpliceExpress.

Authors:  Elena Putscher; Michael Hecker; Brit Fitzner; Peter Lorenz; Uwe Klaus Zettl
Journal:  Int J Mol Sci       Date:  2021-05-13       Impact factor: 5.923

10.  Introduction of Modified BglBrick System in Lactococcus lactis for Straightforward Assembly of Multiple Gene Cassettes.

Authors:  Tina Vida Plavec; Tim Ključevšek; Aleš Berlec
Journal:  Front Bioeng Biotechnol       Date:  2021-12-10
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.