| Literature DB >> 12679813 |
Jérôme Reboul1, Philippe Vaglio, Jean-François Rual, Philippe Lamesch, Monica Martinez, Christopher M Armstrong, Siming Li, Laurent Jacotot, Nicolas Bertin, Rekin's Janky, Troy Moore, James R Hudson, James L Hartley, Michael A Brasch, Jean Vandenhaute, Simon Boulton, Gregory A Endress, Sarah Jenna, Eric Chevet, Vasilis Papasotiropoulos, Peter P Tolias, Jason Ptacek, Mike Snyder, Raymond Huang, Mark R Chance, Hongmei Lee, Lynn Doucette-Stamm, David E Hill, Marc Vidal.
Abstract
To verify the genome annotation and to create a resource to functionally characterize the proteome, we attempted to Gateway-clone all predicted protein-encoding open reading frames (ORFs), or the 'ORFeome,' of Caenorhabditis elegans. We successfully cloned approximately 12,000 ORFs (ORFeome 1.1), of which roughly 4,000 correspond to genes that are untouched by any cDNA or expressed-sequence tag (EST). More than 50% of predicted genes needed corrections in their intron-exon structures. Notably, approximately 11,000 C. elegans proteins can now be expressed under many conditions and characterized using various high-throughput strategies, including large-scale interactome mapping. We suggest that similar ORFeome projects will be valuable for other organisms, including humans.Entities:
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Year: 2003 PMID： 12679813 DOI： 10.1038/ng1140
Source DB: PubMed Journal: Nat Genet ISSN： 1061-4036 Impact factor: 38.330