| Literature DB >> 29197585 |
Cecilia Pinto1, Deni Mance1, Manon Julien1, Mark Daniels1, Markus Weingarth1, Marc Baldus2.
Abstract
Significant progress has been made in obtaining structural insight into the assembly of the β-barrel assembly machinery complex (BAM). These crystallography and electron microscopy studies used detergent as a membrane mimetic and revealed structural variations in the central domain, BamA, as well as in the lipoprotein BamC. We have used cellular solid-state NMR spectroscopy to examine the entire BamABCDE complex in native outer membranes and obtained data on the BamCDE subcomplex in outer membranes, in addition to synthetic bilayers. To reduce spectral crowding, we utilized proton-detected experiments and employed amino-acid specific isotope-labelling in (13C, 13C) correlation experiments. Taken together, the results provide insight into the overall fold and assembly of the BAM complex in native membranes, in particular regarding the structural flexibility of BamC in the absence of the core unit BamA.Entities:
Keywords: BAM; E. coli; MAS; Membrane protein complex; Solid-state NMR
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Year: 2017 PMID： 29197585 DOI： 10.1016/j.jsb.2017.11.015
Source DB: PubMed Journal: J Struct Biol ISSN： 1047-8477 Impact factor: 2.867