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Literature DB >> 29080263

Genomes in Focus: Development and Applications of CRISPR-Cas9 Imaging Technologies.

Spencer C Knight¹, Robert Tjian^2,3,4,5, Jennifer A Doudna^1,2,3,4,6,7.

Abstract

The discovery of the CRISPR-Cas9 endonuclease has enabled facile genome editing in living cells and organisms. Catalytically inactive Cas9 (dCas9) retains the ability to bind DNA in an RNA-guided fashion, and has additionally been explored as a tool for transcriptional modulation, epigenetic editing, and genome imaging. This Review highlights recent progress and challenges in the development of dCas9 for imaging genomic loci. The emergence and maturation of this technology offers the potential to answer mechanistic questions about chromosome dynamics and three-dimensional genome organization in vivo.

Entities: Chemical

Keywords: 3D genome organization; CRISPR; genome editing; imaging; microscopy

Mesh：

Substances：

Year: 2018 PMID： 29080263 PMCID： PMC6014596 DOI： 10.1002/anie.201709201

Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336

104 in total

Review 1. Fluorescence in situ hybridization: applications in cytogenetics and gene mapping.

Authors: B J Trask
Journal: Trends Genet Date: 1991-05 Impact factor: 11.639

Review 2. Biology and Applications of CRISPR Systems: Harnessing Nature's Toolbox for Genome Engineering.

Authors: Addison V Wright; James K Nuñez; Jennifer A Doudna
Journal: Cell Date: 2016-01-14 Impact factor: 41.582

Review 3. Histone modifications and nuclear architecture: a review.

Authors: Eva Bártová; Jana Krejcí; Andrea Harnicarová; Gabriela Galiová; Stanislav Kozubek
Journal: J Histochem Cytochem Date: 2008-05-12 Impact factor: 2.479

Review 4. DNA repair mechanisms in dividing and non-dividing cells.

Authors: Teruaki Iyama; David M Wilson
Journal: DNA Repair (Amst) Date: 2013-05-16

5. Breaking the code of DNA binding specificity of TAL-type III effectors.

Authors: Jens Boch; Heidi Scholze; Sebastian Schornack; Angelika Landgraf; Simone Hahn; Sabine Kay; Thomas Lahaye; Anja Nickstadt; Ulla Bonas
Journal: Science Date: 2009-12-11 Impact factor: 47.728

6. Genome-wide specificity of DNA binding, gene regulation, and chromatin remodeling by TALE- and CRISPR/Cas9-based transcriptional activators.

Authors: Lauren R Polstein; Pablo Perez-Pinera; D Dewran Kocak; Christopher M Vockley; Peggy Bledsoe; Lingyun Song; Alexias Safi; Gregory E Crawford; Timothy E Reddy; Charles A Gersbach
Journal: Genome Res Date: 2015-05-29 Impact factor: 9.043

7. A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture.

Authors: Henriette O'Geen; Isabelle M Henry; Mital S Bhakta; Joshua F Meckler; David J Segal
Journal: Nucleic Acids Res Date: 2015-02-20 Impact factor: 16.971

8. Live Cell Imaging Reveals the Dynamics of Telomerase Recruitment to Telomeres.

Authors: Jens C Schmidt; Arthur J Zaug; Thomas R Cech
Journal: Cell Date: 2016-08-11 Impact factor: 66.850

9. High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity.

Authors: Vikram Pattanayak; Steven Lin; John P Guilinger; Enbo Ma; Jennifer A Doudna; David R Liu
Journal: Nat Biotechnol Date: 2013-08-11 Impact factor: 54.908

10. DNA interrogation by the CRISPR RNA-guided endonuclease Cas9.

Authors: Samuel H Sternberg; Sy Redding; Martin Jinek; Eric C Greene; Jennifer A Doudna
Journal: Nature Date: 2014-01-29 Impact factor: 49.962

21 in total

1. 3D-FISH Analysis of the Spatial Genome Organization in Skin Cells in Situ.

Authors: Andrei N Mardaryev; Michael Y Fessing
Journal: Methods Mol Biol Date: 2020

2. Cas9 interrogates DNA in discrete steps modulated by mismatches and supercoiling.

Authors: Ivan E Ivanov; Addison V Wright; Joshua C Cofsky; Kevin D Palacio Aris; Jennifer A Doudna; Zev Bryant
Journal: Proc Natl Acad Sci U S A Date: 2020-03-02 Impact factor: 11.205

3. CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci.

Authors: Shiqi Mao; Yachen Ying; Xiaotian Wu; Christopher J Krueger; Antony K Chen
Journal: Nucleic Acids Res Date: 2019-11-18 Impact factor: 16.971

4. A CRISPR/molecular beacon hybrid system for live-cell genomic imaging.

Authors: Xiaotian Wu; Shiqi Mao; Yantao Yang; Muaz N Rushdi; Christopher J Krueger; Antony K Chen
Journal: Nucleic Acids Res Date: 2018-07-27 Impact factor: 16.971

5. Genome oligopaint via local denaturation fluorescence in situ hybridization.

Authors: Yanbo Wang; Wayne Taylor Cottle; Haobo Wang; Xinyu Ashlee Feng; John Mallon; Momcilo Gavrilov; Scott Bailey; Taekjip Ha
Journal: Mol Cell Date: 2021-03-02 Impact factor: 17.970

Review 6. Close to the edge: Heterochromatin at the nucleolar and nuclear peripheries.

Authors: Aizhan Bizhanova; Paul D Kaufman
Journal: Biochim Biophys Acta Gene Regul Mech Date: 2020-12-08 Impact factor: 4.490

7. Genome-Scale Imaging of the 3D Organization and Transcriptional Activity of Chromatin.

Authors: Jun-Han Su; Pu Zheng; Seon S Kinrot; Bogdan Bintu; Xiaowei Zhuang
Journal: Cell Date: 2020-08-20 Impact factor: 66.850

8. Live imaging and tracking of genome regions in CRISPR/dCas9 knock-in mice.

Authors: Jinzhi Duan; Guangqing Lu; Yu Hong; Qingtao Hu; Xueying Mai; Jing Guo; Xiaofang Si; Fengchao Wang; Yu Zhang
Journal: Genome Biol Date: 2018-11-08 Impact factor: 13.583

Review 9. CRISPR-Cas guides the future of genetic engineering.

Authors: Gavin J Knott; Jennifer A Doudna
Journal: Science Date: 2018-08-31 Impact factor: 47.728

10. Photocontrol of CRISPR/Cas9 function by site-specific chemical modification of guide RNA.

Authors: Yang Wang; Yan Liu; Fan Xie; Jiao Lin; Liang Xu
Journal: Chem Sci Date: 2020-09-25 Impact factor: 9.825