| Literature DB >> 30166482 |
Gavin J Knott1, Jennifer A Doudna2,3,4,5,6.
Abstract
The diversity, modularity, and efficacy of CRISPR-Cas systems are driving a biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to manipulate the genomes of cultured cells, animals, and plants, accelerating the pace of fundamental research and enabling clinical and agricultural breakthroughs. We describe the basic mechanisms that set the CRISPR-Cas toolkit apart from other programmable gene-editing technologies, highlighting the diverse and naturally evolved systems now functionalized as biotechnologies. We discuss the rapidly evolving landscape of CRISPR-Cas applications, from gene editing to transcriptional regulation, imaging, and diagnostics. Continuing functional dissection and an expanding landscape of applications position CRISPR-Cas tools at the cutting edge of nucleic acid manipulation that is rewriting biology.Entities:
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Year: 2018 PMID： 30166482 PMCID： PMC6455913 DOI： 10.1126/science.aat5011
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728